摘要:

Interaction between polyamide microcapsules having different balances of negative and positive charges on their surface, that is, different isoelectric points and copolymers with different numbers of polyamine macromer of a definite chain length grafted on poly(2-hydroxyethyl methacrylate) backbone was studied by measuring the amount adhered of the microcapsules onto the surface of copolymer-coated glass beads at different pH values. Maximum microcapsule adhesion was observed for a proper combination of microcapsule and copolymer to suggest that separation of a specified cell population from others on the surface of copolymer-coated glass beads can be explained in terms of preferential adhesion through electrostatic interaction of the specified cells with the copolymer.

ISSN:0920-5063    

DOI:10.1163/156856292X00376

出版商:Taylor & Francis Group    

年代:1992

数据来源: Taylor

摘要:

In this study a new dynamic method is introduced allowing the estimation of blood cell adhesion on flat test surfaces by measuring the cell loss in the bulk phase of surface contacting test blood under defined rheological conditions. This was achieved by constructing a novel test chamber permitting the contact of small amounts of blood with a large geometrical test surface. The construction consists of a spiral-shaped flow channel of 0.3 cm width, 0.02 cm height and 78 cm length covered with the biomaterials to be tested from both sides. Laminarity of blood flow in the conduit was confirmed theoretically by the calculation of an equivalent to the Reynolds number for curved systems the so-called Dean number. Furthermore, flow laminarity was proved experimentally finding that the flow rate of blood with different hematocrit values was proportional to the hydrostatic pressure applied. The applicability of the novel 'spiral method' for the estimation of hemocompatibility was demonstrated by evaluation of platelet adhesion onto different polymers in comparison to siliconized and fibrinogen coated glass as reference surfaces. Additionally, it was possible under distinct conditions to determine the adhesion of leucocytes and the detachment of platelet aggregates. Therefore, it was concluded that the spiral method can be used for the assessment of the hemocompatibility of flat biomedical polymers. As main advantages of the new method can be considered the high time efficiency and accuracy without labelling or optical detection of adherent cells.

ISSN:0920-5063    

DOI:10.1163/156856292X00385

出版商:Taylor & Francis Group    

年代:1992

数据来源: Taylor

摘要:

Connective tissue substitute can be made of collagen sponge-shape matrice which is reconstituted by freeze-drying a collagen dispersion. This procedure is then followed by a crosslinking treatment to decrease the in vivo biodegradation rate. In the present study, collagen dispersions made of collagen fibrils with a D-staggered pattern were submitted to the following treatments: (1) cyanamide or glutaraldehyde was introduced during the dispersion step followed by the manufacture of sponges; (2) uncrosslinked sponges were exposed to formaldehyde vapor; or (3) uncrosslinked and crosslinked sponges were severely dehydrated. To characterize the in vitro contraction rate, the surface areas of sponges were sequentially recorded in relation to soaking time. Contraction did not significantly occur when sponges were chemically treated. However, collagen in sponges treated by either severe dehydration or by both cyanamide treatment and severe dehydration contracted. On the other hand, the different treatments of the collagen modified the distribution of the D-staggered pattern within fibrils. After glutaraldehyde treatment, the periodicity of collagen fibrils disappeared and large fibres were observed. These experiments show that the different treatments of the collagen can be useful for designing a contractile as well as a non-contractile biomaterial.

ISSN:0920-5063    

DOI:10.1163/156856292X00394

出版商:Taylor & Francis Group    

年代:1992

数据来源: Taylor

摘要:

This paper reviews the development of the polyanhydrides as bioerodible polymers for drug delivery applications. The topics include design and synthesis of the polymer, physical properties, techniques to fabricate the polymer into drug delivery devices, evaluation of biocompatibility, and example applications of the polyanhydrides. Discussion of the interrelationship between the physical-chemical properties of the polyanhydrides, fabrication methods, and drug release rates is included. One section is devoted to a case study to provide a historical perspective of the development a polyanhydride-based drug delivery treatment from the conception of the idea to the final stages of human clinical trials. This section includes an outline of the extensive in vitro and in vivo testing that is necessary for development of a new material for biomedical applications.

ISSN:0920-5063    

DOI:10.1163/156856292X00402

出版商:Taylor & Francis Group    

年代:1992

数据来源: Taylor