摘要:

AbstractThe antheridiogen (antheridium‐inducing substance) of the fern speciesAnemia phyllitidishas been obtained in pure form based on the isolation procedure described below. Pure antheridiogen is active to a dilution of 10 μg/l in antheridium formation and 0.3 μg/l in dark‐germination. Its molecular formula is C19

ISSN:0031-9317    

DOI:10.1111/j.1399-3054.1972.tb03565.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

AbstractThe effect of NAA (1‐naphthaleneacetic acid) and morphactin (methyl 2‐chloro‐9‐hydroxyfluorene‐9‐carboxylate) on the uptake and incorporation of uridine‐5‐3H andl‐leucineU‐14C was studied in senescing bean endocarp (Phaseolus vulgarisL. cv. Kentucky wonder pole beans) sections. Both growth regulators inhibited uptake of these precursors and appeared to affect different components of the uptake mechanism. A possible synergistic inhibition was seen in case of leucine, but in case of uridine, the effect was less than additive.A strong synergistic increase in the incorporation ofl‐leucine into protein was seen. Thus, morphactin interacted with an exogenously applied auxin at the level of the protein s

ISSN:0031-9317    

DOI:10.1111/j.1399-3054.1972.tb03566.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

AbstractFreshly isolated spinach (Spinacea oleracea) chloroplasts were treated with 4 different neutral salt solutions at concentrations of 1 and 2Mfor ultrastructural study. Each salt induces a specific pattern of structural disorganization. In KCl, there is principally swelling of the fret‐canal and end‐granal‐compartment loculi. With KI, there is removal of green pigment, loss of some stainability, swelling, and separation of the individual membranes of the granal partition layers. Sodium trichloracetate causes apparent end‐to‐end fusion of granal stacks and their curvature; and in KSCN there is a great volume increase, related to the separation of the membranes of the partition region and extensive swelling of all the loculi.This work has been rendered possible through a grant from the National Science Foundation (

ISSN:0031-9317    

DOI:10.1111/j.1399-3054.1972.tb03567.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

AbstractThe ATPase activity of the cell wall, mitochondrial and microsomal fractions of bean roots (Phaseolus vulgaris) shows non‐linear curves when plotted logarithmically against temperature, producing high Q10‐values in the low temperature range and low Q10‐values in the high temperature range. The Q10‐valuc of supernatant ATPase is constant and low as well as the Q10‐value of soluble ATPase liberated from membranes. Cell wall ATPase from bean roots adapted to a low temperature has a much smaller temperature response. Such roots contain a larger quantity of membrane‐bound ATPase and a smaller amount of soluble ATPase than roots adapted to a high temperature.Extraction of soluble ATPase with acetone reduces activity to practically zero, but activity could be partially restored by the addition of lipid. The temperature response of reconstituted phosphatidyl choline‐ATPase is inconsiderable. That of reconstituted sulfolipid‐ATPase is much larger.I am indebted to Bep Stuiver for skillful assistance with the experiments, to Ir. F. Kuiper and Mr. C. H. Vermeulen for help with the cultivation of the bean plants and to Prof. Dr. E. C. Wassink for reading the manuscript.Communication 292 of the Laboratory of Plant Physiological Research, Agricultural University, Wageningen,

ISSN:0031-9317    

DOI:10.1111/j.1399-3054.1972.tb03568.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

AbstractWhite light increases the inhibition of growth in length of pea seedling roots caused by 2,4‐D. This effect is most pronounced in the case of lateral root growth. The effect is obtained also if the shoots above the cotyledons are removed and is probably due to the direct influence of light on the roots. While light also weakly enhances the inhibition of pea root growth caused by 1‐naphthylacetic acid, light does not increase the sensitivity of wheat roots to 2,4‐D or naphthylacetic

ISSN:0031-9317    

DOI:10.1111/j.1399-3054.1972.tb03569.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

AbstractYoung peach plants (Prunus persica) were grown outdoors under different colored filters, to examine the effect of light quality on plant behavior. It was found that under blue light growth rate, leaf size and number, rate of spring bud opening and secondary branching were very similar to control plants grown under neutral shade. Blue + far‐red light showed an overall strong inhibitory effect on all these characteristics. Red + far‐red light produced the strongest growth activity with best results in growth rate and leaf size and number. The phytochrome pigment system was suggested to be the only pigment regulating growth under high light intensities.Blue and blue + far‐red light acted antagonistically on apical dominance features of the tree. The former produced a wider tree with nearly horizontal shoots, while the latter produced a more erect narrow

ISSN:0031-9317    

DOI:10.1111/j.1399-3054.1972.tb03570.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

AbstractThe relationship between relative humidity and transpiration was studied in the basidiocarps ofCoprinus, Amanita, Agaricus, Tricholoma, CollybiaandPolyporus. Transpiration decreases with an increase in the humidity and there is virtually no transpiration in nearly saturated air.To determine the relationship between uptake and transpiration, stipes were immersed in solutions of various osmotic ally active substances and inhibitors. Results showed that uptake has no influence on the transpiration.Additional data suggested that uptake in a detached basi‐diocarp proceeds as long as the fruit body is young and healthy. Studies with fluorescein dye and mannitol showed that most transpiration occurs at the surface and edges of the hymeniu

ISSN:0031-9317    

DOI:10.1111/j.1399-3054.1972.tb03571.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

AbstractThe bacteriolytic enzymes produced byMyxococcus virescensand previously concentrated and separated from most of the non‐bacteriolytic proteins have been further separated and purified.The bacteriolytic enzyme solution was concentrated by lyo‐philization. When applied to a Sephadex G‐100 column, three peaks of bacteriolytic activity were eluted. Polyacrylamide gel electrophoresis showed that all the three enzyme fractions were contaminated with at least four non‐bacteriolytic proteins. In the first enzyme fraction the bacteriolytic enzymes could be freed from the contaminating proteolytic activity by adsorption on a hydroxylapatite column. The bacteriolytic enzymes could then be adsorbed on a CM‐cellulose column. The remaining contaminating proteins passed the column un‐adsorbed while the bacteriolytic enzymes could be eluted with a gradient of 0.02–0.10Mammonium hydrogen carbonate solution. The second enzyme fraction was adsorbed on a CM‐cellulose column and then eluted with 0.03–0.15MNH4 HCO3. After rechromatography on a new column under the same conditions, all of the contaminating proteins had disappeared. For purification of the third enzyme fraction chro‐matography on one single CM‐cellulose column was sufficient. The elution of the adsorbed enzymes was performed with a gradient of 0.15–0.30MNH4HCO3. The recovery of activity for each of the ion‐exchange chromatography separations was at least 90%. The purity of the enzymes was tested by polyacrylamid gel electrophoresis. Each of the purified enzymes gave only one coloured band which coincided with the enzyme activity assayed in sliced gels. The molecular weights of the enzymes were determined by electrophoresis on acryl‐amide gels containing sodiumdodecylsulphate. The molecular weights determined in this way (about 40,000, 30,000 and 20,000, respectively) were about 10,000 daltons higher than those obtained by gel chromatography on Sephadex G‐100. This discrepancy seems to depend on interactions between the enzymes and the dextran molecules probably caused by the strongly basic nature of the enzymes or by formation

ISSN:0031-9317    

DOI:10.1111/j.1399-3054.1972.tb03572.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

AbstractNicotinamide nucleotide coenzymes were estimated enzymatically in cucumber leaves (Cucumis sativusL. cv. Suisei No. 2) during ammonium toxicity. The contents of all the coenzymes (NAD(H) and NADP(H)) were found to be higher in the ammonium‐treated plants than in the control plants, and the difference attained a maximum at 5 days after the initiation of ammonium treatment. Thereafter, the contents of NAD and NADH returned towards the control level, but NADP and NADPH levels were lowered in injured plants. The ratios of NAD/NAD + NADH and NADP/NADP ++ NADPH were little altered by the ammonium treatment.Changes of nicotinamide nucleotide coenzymes are discussed in relation to respiratory metabolism in cucumber leaves during ammonium toxicit

ISSN:0031-9317    

DOI:10.1111/j.1399-3054.1972.tb03573.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

AbstractThe effect of red light (660 nm), far‐red light (730 nm) and dark treatment on the subsequent enzyme activity in homogenates of Avena coleoptiles was investigated. The activities of succinic dehydrogenase (SDH), lactic dehydro‐genase (LDH) and glucose‐6‐P dehydrogenase (GDH) were investigated. The activity of SDH was greatest in material receiving continuous darkness. LDH and GDH activity was stimulated by both light treatments compared with the dark values. Little or no difference in enzyme activity was found using either a single 15 min flash of light or continuous light for 24 h. Admixtures of extracts from dark treated and light treated material in a 1:1 ratio gave unexpected levels of enzyme activity. In all cases such admixtures gave much less than the anticipated enzyme a

ISSN:0031-9317    

DOI:10.1111/j.1399-3054.1972.tb03574.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY