ISSN:0935-6304    

DOI:10.1002/jhrc.1240020902

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1979

数据来源: WILEY

摘要:

AbstractAn automatic injection system for the analysis of gas phase smoke has been designed and evaluated using the microprocessor‐controlled Hewlett‐Packard 5830 gas chromatograph. The cryogenic preconcentration trap was constructed from relatively inexpensive and readily available materials. It was determined that the use of the preconcentration trap, as opposed to condensing the smoke on the head of the cold (‐50°C) chromatographic column, significantly enhanced the resolution of the more volatile gas phase components. Data are presented which illustrate the injection system characteristics. In addition, chromatograms of gas phase smoke are presented which demonstrate the effectiveness of this tec

ISSN:0935-6304    

DOI:10.1002/jhrc.1240020903

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1979

数据来源: WILEY

摘要:

AbstractStudies of the pharmacology and toxicology of the popular insect repellant, N,N‐diethyl‐m‐toluamide (DEET), have largely been done in animal models using radioactive tracing without the structural elucidation of its metabolites.This paper describes a high resolution gas chromatography/mass spectrometry (GC/MS) technique and reports the results of the preliminary characterization of the metabolites of DEET in the urine of a 30‐year‐old man who had been exposed to DEET contained in a commercial product. The metabolites were extracted and separated with an OV‐101 glass capillary column, 30 m × 0.3 mm, and mass spectrometric elucidations were carried out with both Electron Impact (EI) and Chemical Ionization‐Methane (MCI) modes.Oxidation of the benzylic moiety and hydroxylation of the sidechain of DEET molecules appeared to be the predominate routes of metabolism in man. The artifacts were

ISSN:0935-6304    

DOI:10.1002/jhrc.1240020904

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1979

数据来源: WILEY

摘要:

AbstractThe effect of pressure changes in the vaporizing GC injectors on the split ratio is investigated. It is shown that the true split ratio depends on a number of parameters and may strongly differ from the split ratio expected from the flows set before injection. Quantitation with split injection based on external standards has to be carried out very carefully (or should be replaced by the method with internal standard). The split ratio may strongly change during the splitting of the sample. There are a number of mechanisms causing some sample components to be retarded and hence to be split by a different ratio. The split injector is a device which still requires further development.

ISSN:0935-6304    

DOI:10.1002/jhrc.1240020905

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1979

数据来源: WILEY

摘要:

AbstractReversed‐phase high performance liquid chromatography on microparticulate columns of octadecylsilane with elution by a gradient of acetonitrile in phosphate buffer, pH 7.5, separated porphyrin free acids directly in urine for the first time. Interfering fluorescence was removed with a precolumn of large particle size at acid pH. Peak area fluorescence by the method of standard addition was related linearly to amount of a porphyrin from 5 to 60 nanograms. The method was reproducible to ± 13%, able to detect 0.3 nanograms of a porphyrin in a 1 ml urine sample in 45 minutes, and capable of detecting the typical clinical patterns of normal and porphyrinuric urines. Modifications make the method applicable to measurements in other biological samples and to research separations. With the added rapidity of analysis, the method could expand porphyrin analysis in routine clinical medici

ISSN:0935-6304    

DOI:10.1002/jhrc.1240020906

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1979

数据来源: WILEY

ISSN:0935-6304    

DOI:10.1002/jhrc.1240020907

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1979

数据来源: WILEY

ISSN:0935-6304    

DOI:10.1002/jhrc.1240020908

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1979

数据来源: WILEY

ISSN:0935-6304    

DOI:10.1002/jhrc.1240020909

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1979

数据来源: WILEY

ISSN:0935-6304    

DOI:10.1002/jhrc.1240020910

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1979

数据来源: WILEY

ISSN:0935-6304    

DOI:10.1002/jhrc.1240020911

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1979

数据来源: WILEY