ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02159.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02160.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractThe corneal epithelium responds rapidly to injury, repairing defects with a layer of cells that covers the denuded corneal surface and prevents infection and loss of vision. After a wound, reorganization of the remaining epithelial cells occurs over several hours, resulting in the formation of a migratory leading edge. However, expression of genes such as c‐fosoccurs within minutes of wounding. This early expression may be important for directing epithelial reorganization and the later mitotic burst. Our results show that receptors for epidermal growth factor are upregulated in the migratory cell population. Proliferation through a mitotic burst was observed in cells surrounding the original wound margin after 36 hours. The interaction between gene expression and cell surface receptors for growth factors and cell proliferation suggests that wound healing occurs in a complex, but tightly controlled process in the corneal epitheliu

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02161.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractThe corneal epithelium adheres to the stro‐ma through a series of linked structures termed collectively the adhesion complex. These structures include; intermediate filaments (keratin filaments) which are linked to the hemidesmosome; the hemidesmo‐some; the anchoring filaments which extend from the membrane at the hemidesmosome through the lamina lucida to the lamina densa region of the basement membrane; the anchoring fibrils which insert into the basement membrane from the stromal side; and the anchoring plaque on which anchoring fibrils terminate distal from their insertion on the basement membrane. Upon wounding, basal cells of the corneal epithelium disassemble their hemidesmosomes. During migration, the membranes along the wound bed exhibit a different kind of adhesion junction, the focal contact. This junction is present primarily in cells of the leading edge of migration and may be the provisional adhesion junction used by epithelial sheet moving to cover a wo

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02162.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractExtracellular matrix (ECM) proteins form distinct proteinfamilies that play a role during tissue maturation, wound healing and maintenance of tissue architecture. Recent studies show that there are tissue type ‐specific variations in their expression. ECM proteins function by complexing with each other and also by interacting with their cellular receptors, called integrins. Integrins are heterodimeric membrane.glycoproteins that are partly cell type ‐specifically expressed in human tissues. Like other stratified epithelia, corneal epithelium expresses α2β1, α3β1, α6β1,4, and αvβ1integrins that mediate attachment to the basement membrane and cell‐cell

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02163.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractIntegrins are heterodimeric plasma membrane glycoproteins involved in cell‐matrix and cell‐cell interactions. The present communication reviews the distribution of several of the currently known inte‐grin subunits in the corneal epithelium. The corneal epithelium contains the following integrin hetero‐dimers: α2β1, α3β1, and α6β4. The expression of αvwith an unknown β subunit is also recognized, whereas the expression of α4and α5subunits remains controversial. Some of the changes occurring in the distribution of integrins in response to wounding will

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02164.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractWe have documented changes in expression of collagenolytic/gelatinolytic enzymes of the matrix metalloproteinase family (MMP) in healing or ulcerating corneal wounds of rat or rabbit. Correlation of our findings with specific changes in the extracellular matrix of the repair tissue suggests two different roles for the enzymes, MMP‐2 and MMP‐9. MMP‐2 is expressed in undamaged corneal stroma where it may degrade the occasional collagen molecule that becomes damaged. After corneal wounding, expression of this enzyme is increased and much of it appears in the active form. These changes persist for at least seven months, suggesting that MMP‐2 is involved in the prolonged process of collagen remodelling in the stromal repair tissue. MMP‐9 is expressed in the epithelial layer of repair tissue with a timing suggesting it might participate in controlling resynthesis of the basement membrane. MMP‐9 also appears to be involved in degradation of the epithelial basement membrane that precedes corneal

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02165.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02166.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractIn this article we review a novel type of plasminogen activation on staphylococcal and strep‐tococcal cells. The activation mechanism implies a specific binding of glu‐plasminogen to bacterial surface via the lysine‐binding sites of plasminogen. Association of plasminogen with bacterial surfaces greatly enchances the t‐PA mediated activation which takes place only poorly in solution. The end product, surface‐associated plasmin, is enzymatically active, protected against high molecular weight plasmin inhibitors and capable of converting itself from glu‐plasmin to the lys‐form. The modification is associated with an increased affinity of the bound lys‐plasmin towards the binding molecules on bacterial surface. This novel way of retaining plasmin on the surface may be important for the bacteria to invade and penetrate surrounding tissues. Our data on the effect of plasmin on staphylococcal adherence indicate that plasmin is not very effective in cleaning bacteria from surfaces coated with extracellular matrix components, fibronec‐t

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02167.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractThe involvement and role of the plasmino‐gen activator‐plasmin system in normal and pathological wound healing is reviewed. The methods currently available for demonstrating plasmin activity are briefly described. The article also reviews some other serine proteases potentially involved in pathological wound healing processes. The current ophthalmolog‐ical therapeutic measures used to regulate tissue pro‐teolysis are also de

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02168.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY