ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02174.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractThe embryology and natural history of the lens capsule and the zonular apparatus have been described according to the present knowledge of the subject. Clinical evidence pointing towards an active turnover of lens capsule material is presented.

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02175.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractAfter‐cataract can be defined as the retropupillary, non‐vitreal opacification of the lens remnants occurring after extracapsular cataract extraction (ECCE) leading to disturbance of transparency and impairment of vision. The synonymous secondary cataract is confusing, since it is also and more frequently used as cataract secondary to ocular diseases (Duke‐Elder 1969). The synonymous opacification of the posterior capsule is, in fact, a misnomer, since histological observations revealed that after‐cataract is not proven to be the result of changes in the remaining posterior capsule itself, but is caused by proliferation of lenticular epithelial cells, fibroblasts, macrophages and even iris‐derived pigment cells on the posterior capsule

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02176.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractSecondary cataract formation is the most common postoperative complication following cataract surgery. To study different methods preventing the secondary cataract formation a relevant animal model is required. An in vivo rabbit model for studies on the prevention of secondary cataract formation is presented. The lens nucleus and cortex were removed by phacoemulsification, and two months later the growth of the secondary cataract was estimated by scoring on a scale from 0–4, both central and peripheral, and by measuring the wet mass of the dissected secondary cataract. The model seems to be a relevant way to study different concepts for the prevention of secondary cataract formatio

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02177.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractWe cultured the human lens epithelial cells with attaching anterior lens capsule using surgically removed capsular flaps obtained in intraocular lens implantation. The capsular flap was placed with cell side down in a small culture well. In phase contrast microscopy, an anti‐meniscus plate was placed on the bathing medium to avoid optical aberration. The human lens epithelial cells showed outgrowth which ceased after 3 to 4 weeks of incubation. The cells under the capsule lost distinctive cell margin. The cells outgrew from the capsular edge both onto the anterior capsular surface and the well bottom. The outgrowth length and Bromodeoxyuridine uptake indicated a low proliferative potency of the human lens epithelial cell

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02178.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractLens epithelial and other ocular cells contain complex arrays of actin filaments which might be expected to allow them to migrate following injury: specifically into the capsular sac in cases of extracapsular cataract extraction or traumatic cataract. To test the possibility, a culture system was developed using a melanotic strain of mice, in which migrating cells are often ‘marked’ by melanosomes. Injured lenses were cultured on permeable membranes in contact with nutrient medium, and surrounded by iridial tract fragments. After study by light and electron microscopy, it was established that both pigmented and unpigmented cells migrated from the surrounding explants, apparently along the substrate meniscus, to the outside of the lens capsule and then through the wound to the capsule interior. This study suggests a source of cells in development of after‐cataract syn

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02179.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractThe expression of the neural cell adhesion molecule NCAM and its polysialic acid (PSA) moiety was documented during embryonic development and adult differentiation of chicken lens cells. In both the embryo and adult, NCAM is predominantly found in the epithelium and the zone of young elongating cells of the annular pad. NCAM abundance drops markedly in the cortical fibers and is further reduced in the lens nucleus. Epithelial cell NCAM is more highly polysialylated in the adult than in the embryonic lens. Three isoforms of NCAM at 180, 140, and 120 kDa were detected in the lens and predominantly associated with the unit membrane‐enriched plasma membranes of fiber cells. The distribution of NCAM relative to MP26 and the adherence junction‐associated glycoprotein N‐cadherin suggests that NCAM could influence the formation of fiber cell gap junctions and adherence junc

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02180.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractA lens growth factor was identified that is present in the anterior chamber of the embryonic chicken eye. The mitogen is similar to an embryo‐specific activity found in embryo serum. Several purified growth factors, applied singly or in combination, did not stimulate cell division in embryonic lens cells. The serum mitogen is a protein which does not bind to heparin‐Sepharose. The possibility is presented that the lens epithelium contains two distinct cell types, the proliferating cells of the germinative zone and the mitotically quiescent central epithelial cells. It is proposed that only cells in the germinative zone are capable of responding to normal lens growth factors. It is likely, therefore, that these cells present the greatest risk for secondary cataract format

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02181.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractThe reactive production of hyaluronic acid was studied in the rabbit lens following a 4 mm in diameter anterior lens wound, and following a large lens wound (extracapsular cataract extraction). Furthermore, human post‐mortem after‐cataract specimen were also studied. The hyaluronic acid was localized histochemically using a new specific technique. Hyaluronic acid in the small wound was localized in the primary sealing plug that precedes the reepithelialization and new capsule formation. In the large wound hyaluronic acid was found around the cells proliferating on the posterior capsule. The same was true in the human after‐cataract specimen. It was concluded that the primary wound sealing and the posterior capsule cellular outgrowth constitute similar cellular resp

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1992.tb02182.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY