摘要:

The morphological changes in the rabbit cornea due to a linear wound and a central keratectomy and its reepithelization were examined under the scanning electron microscope. After the rounding off and retraction of the edges of the wound, during the first 6 h, the stroma was infiltrated by leucocytes. First of all the superficial epithelial cells migrated over the edges of the wound to protect the exposed cleft in the tissue. After 15 h the basal epithelial cells sent pseudopodia‐like processes of varying lengths into the wound area. The migrating cells were mainly flat. During this phase the primary migration of the basal epithelial cells was followed by subsequent cell layers. In the migration, three types of cells can be distinguished: 1. very flat cells with smooth edges without microprojections, which adapt their shape to the clefts in the damaged stroma, 2. flat cells with considerably folded plasma membrane on the cell surface giving a coral‐like appearence, 3. cells with voluminous cytoplasmic processes. The rapidly sliding epithelium covered the defect with star shaped growths so that after only 48 h a nearly complete closure of the wound was achieved. After 96 h the deep wound was completely covered by epithelium. The epithelial cells did not yet possess their original surface structure in that they had demonstrably fewer microvilli and microplicae than normal. Only after five days at the earliest could the normal surface relief be seen. The surface cells were predominant in the first stage of the healing process and the basal cells only in the second st

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1979.tb00525.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Using reservoirs containing 50 μl of antigen solution in combination with a micro‐modification of the electroimmunoassay a detection limit of 0.05 ng applied in 400 μl corresponding to 0.125 ng/ml has been achieved.Tear fluid eluate from Schirmer paper strips has been tested in the assay. The eluation procedure failed to extract all tear proteins as 32 ng were left in the paper. Despite the loss, experiments have shown the method to be suitable for immunological determination of tear proteins colleced by the Shirmer I t

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1979.tb00526.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Endothelia of 102 clear human corneal grafts in 95 patients were photographedin vivowith a specular microscope. The relation of the endothelial cell density to the HLA and ABO compatibility of the donor‐recipient pairs were studied. In addition, the effects of vascularization and reversible rejection on the endothelial cell density were analysed.A negative correlation was found between the HLA incompatibility and the endothelial cell density of the graft. The group with 0–1 (2) mismatches had the highest endothelial cell density (1356 ± 510 cells/mm2). This result was better than in the group with 2 (3) mismatches, but the cell difference was not statistically significant. A statistically significant difference in average endothelial cell densities was found between the groups with 0–1 (2) mismatches and with 3–4 mismatches, the latter having 880 ± 475 cells/mm2(P<0.01). There was also a statistically significant difference in average endothelial cell densities between the group with 0–1 mismatch and the untyped group (P<0.01). The difference in cell densities between the group with 2 (3) mismatches and the more incompatible groups together (3–4 mismatches and the untyped group) was statistically almost significant (P<0.05).The ABO‐compatible pairs had 18% more endothelial cells than the ABO‐incompatible pairs. The difference was not statistically significant, but when the ABO‐compatible pairs were compared with all the others (incompatible and untyped), the difference in mean cell density between the two groups was statistically almost significant (P<0.05).Comparison of the effect of tissue incompatibility on graft endothelial cell density separately in the patients with vascularized and non‐vascularized corneas showed that in both categories the endothelial cell density was highest in the group with 0–1 (2) mismatches.In 8 patients with a reversible rejection episode the average cell density was 20% less than in those with an uneventful postoperative period. The importance of rejection on the endothelium in some spe

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1979.tb00527.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Evaluation of the results of HLA typing of 187 patients grouped into herpetic keratitis (37), non‐herpetic keratitis (43), keratoconus (42), endothelial dystrophy (23), stromal dystrophy (13), lues (5), and injuries (24), failed to show convincing deviations in any of the groups from a normal control series (2900 persons). Yet, as for the herpes group, a rise in B5 must strongly be suspected. The data are presented and possible implications are discusse

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1979.tb00528.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The non‐contact specular microscope enables a large area of the corneal endothelium to be examined and photographed without any risk of traumatizing the anterior surface of cornea. This non‐contact technique is described in detail. Standard deviation of the relative difference between two independent estimates of central endothelial density from 24 eyes was 3.7%. Standard deviation of the relative difference between counts from left and right eyes from 57 subjects was 4.7%. A significant negative correlation between age and endothelial cell density was found (r = ‐0.59,P<0.001, n = 76). The interindividual variation in cell densities was found to be larger in the old age group, indicating that cell loss during aging varies among different indivi

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1979.tb00529.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Rabbit corneal endothelial permeability to sucrose and dextran was determined after storage in moist chamber or MK medium. When compared to fresh rabbit corneas there was no statistically significant difference in endothelial permeability to these two solutes after both types of storage for periods up to ten days.

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1979.tb00530.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The corneal endothelial cells of 25 patients with unilateral acute glaucoma were photographed with a clinical specular microscope. Photography was performed as soon as (average 6–12 h) the IOP had been lowered with iv Acetazolamide and/or Mannitol and topical Pilocarpine therapy, and the corneas had become clear. Peripheral iridectomy was performed on the affected eye and prophylactically on the fellow eye in most of these patients. The follow‐up endothelial photographs were taken 6–24 months postoperatively.High intraocular pressure lasting 3 days or more lowered the central endothelial cell density. But a rise in pressure lasting from only a few hours to 2 days did not affect the endothelial cell count. Operative glaucoma procedures caused a loss of central endothelial cells of approximately 4.8% in the series. There was a clear correlation between the duration of elevated pressure and the number of central corneal endothelial cells

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1979.tb00531.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The central corneal endothelium of 22 normal subjects and 11 unilateral aphakic subjects was photographed with the non‐contact specular microscope. The aphakic patients had undergone intracapsular cataract extraction one to four years previously. Endothelial cell densities were estimated. All cells which had been counted were then grouped according to their number of neighbours. The percentages of cells having 4, 5, 6, 7, or 8 neighbours were means ± SD): 0.4 ± 0.8; 18.0 ± 3.4; 64.1 ± 6.5; 15.9 ± 2.6 and 0.9 ± 0.8 per cent, respectively in the normal eyes, and 0.8 ± 1.3; 20.9 ± 3.7; 57.4 ± 5.6; 19.6 ± 3.9 and 1.4 ± 1.2 per cent in the aphakic group, respectively. The frequency of cells having 5, 6, or 7 neighbours were significantly different in the two groups. Cells with 9 or 10 neighbours were not seen in the normal group but occurred in 2 and 1 of the aphakic eyes, respectively. In the normal group the frequency of hexagonal cells was found to correlate to the cell density (r = 0.46,2P<0.01) and age (r =

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1979.tb00532.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The specular microscope was used to study the corneal endothelium in three patients with essential iris atrophy and three patients with anterior mesodermal disorders with uncertain diagnose. Pleomorphic and enlarged endothelial cells were found to be typical for essential iris atrophy. The changes were also present in one eye with normal pressure.In the patients with other types of iridal disorder the endothelial cells were normal in form. In one of these patients high intraocular pressure and surgery had seemingly led to enlargement of the endothelial cells. According to our findings, the specular microscope is a useful aid in differentiating essential iris atrophy from other disorders of the iris.

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1979.tb00533.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Siamese cats are albinos with poor visual resolution and severely impaired binocular vision. Eye muscle physiology was studied in Siamese cats as a part of a more extensive project on eye muscle properties in cats with deficient binocular vision. Isometric contractions of the inferior oblique muscle were recorded in response to single and repetitive muscle nerve stimulation. Speed of contraction, measured as twitch contraction time, fusion frequency and rate of tetanic tension rise, was lower in Siamese than in normal cats. Eye muscles of Siamese cats fatiqued more easily to continuous activation than normal cat eye muscle. These functional changes have also been found in cats with binocular defects from monocular lid suture, but were much more marked in Siamese cats. It is suggested that the eye muscle changes represent muscular adaptations to genetically caused impairments of binocular vision and visual resolution in Siamese cats.

ISSN:1755-375X    

DOI:10.1111/j.1755-3768.1979.tb00534.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY