ISSN:0893-0341    

出版商:OVID    

年代:1992

数据来源: OVID

ISSN:0893-0341    

出版商:OVID    

年代:1992

数据来源: OVID

摘要:

Nonneural tissues are now widely used to search for abnormalities in genes as well as for other markers of dementia of the Alzheimer type (DAT). Studies of nonneural tissues can experimentally circumvent problems inherent in the study of autopsy brain, but to be meaningful, abnormalities identified in the periphery must be correlated with abnormalities in the brain, which is the tissue of clinical interest. Among the topics in DAT research that can be readily studied in nonneural cells (including tissue cultures) are molecular genetics, amyloid precursor protein formation and metabolism, systemic manifestations of immunological and inflammatory mechanisms, proteolysis, membranes, signal transduction, and mitochondria and metabolism. Although phenomena suggesting the possibility of cytoskeletal abnormalities in nonneural DAT cells have been described, the τ molecules involved in paired helical filament formation are relatively brain-specific. Since the neuropathological diagnosis of DAT depends on recognizing apatternof changes rather than any single abnormality, it seems unlikely that any one laboratory abnormality in peripheral tissues will correlate precisely with the clinicopathological entity of DAT. However, abnormalities found in nonneural DAT cells that correlate with the existence of similar abnormalities in the brain are likely to be informative about the disease process in the patients in whom they occur.

ISSN:0893-0341    

出版商:OVID    

年代:1992

数据来源: OVID

摘要:

β/A4, a peptide that forms the extracellular amyloid fibrils of Alzheimer senile plaques, has also been proposed to be a component of Alzheimer paired helical filaments (PHFs). We compared BR88, an anti-serum to amino acids 1–12 of β/A4, with BR126, an antiserum to the sequence SEKLDFKDRVQS in τ protein, since τ protein is the only confirmed component of PHFs. In enzyme-linked immunosorbent assays (ELISAs), both antibodies reacted with pronase-treated PHFs better after PHFs were treated with guanidine. τ protein shares no sequence homology with β/A4. Nevertheless, BR88 cross-reacted with human recombinant τ isoforms by ELISA and Western blot analysis with potencies comparable to those for anti-τ antibodies. BR88 reacted with a β/A4 peptide as well on a molar basis as with τ protein and showed some reactivity to the tubulin-binding region of τ protein. In conclusion, the β/A4 antiserum BR88 cross-reacts with τ protein, possibly explaining its reactivity with PHFs.

ISSN:0893-0341    

出版商:OVID    

年代:1992

数据来源: OVID

摘要:

The Extended Scale for Dementia (ESD), a development of the Mattis Dementia Rating Scale, has been used in the evaluation of dementia and aging and has shown substantial clinical utility. We report on analyses of its properties and internal structure in three samples of older people: 153 normals, 101 psychiatric hospital residents, and 114 patients with Alzheimer disease. The results showed good internal consistency in the two clinical samples, with much lower reliability in the normals, for whom the test was too easy. A review of the item statistics led to the use of 17 of the 23 ESD items in item component analyses in the three samples. Use of Horn's parallel analysis criterion led to the retention of three components in the normal group and one in both the hospital and Alzheimer groups. The results are compared with other work and are in accordance with the view that cognitive structure becomes more simple with increasing dementia.

ISSN:0893-0341    

出版商:OVID    

年代:1992

数据来源: OVID

ISSN:0893-0341    

出版商:OVID    

年代:1992

数据来源: OVID

ISSN:0893-0341    

出版商:OVID    

年代:1992

数据来源: OVID

ISSN:0893-0341    

出版商:OVID    

年代:1992

数据来源: OVID

ISSN:0893-0341    

出版商:OVID    

年代:1992

数据来源: OVID