摘要:

Three block sectionsof treated human intraosseous lesions within one individual are presented. These lesions were debrided, filled with autogenous grafts and at two sites the root was treated with citric acid. The treated sites were lightly root planed once a month and the blocks were removed 6 months after surgery. In addition, the root accretions within two lesions were notched with a bur prior to root planing in order to positively identify the position of these root accretions in the histologic sections. Clinically, the lesions closed by marginal shrinkage and limited pocket closure. Histologically, regeneration of new cementum, osteogenesis at bone and graft spicule seams and reformation of functionally oriented ligament fibers were present at sites within lesions where accretions had covered the root. Such a healing phenomenon was observed close to the base of the lesions and seemed to be related to this spatial configuration rather than to the notching of the root itself. No specific increased healing responses could be attributed to citric acid root treatment. Thus, “regeneration” of new attachment is possible within human intraosseous lesions even at root sites previously covered by accretions. However, such responses seem to be limited to areas near the base of the intraosseous lesion.

ISSN:1049-8885    

DOI:10.1902/jop.1983.54.2.67

出版商:Wiley    

年代:1983

数据来源: WILEY

摘要:

This studyevaluated five agents for their ability to detoxify periodontally‐involved root surfaces. An ultrasonic scaler was used to remove all visible calculus from a proximal surface of 250 teeth extracted because of advanced periodontal disease. The teeth were then randomly divided into five groups of 50 each. One‐half of a proximal surface of each tooth in each group was considered a control and was treated by rubbing the surface continuously for 3 to 5 minutes with cotton pellets soaked in sterile saline. The control area was then removed with a sterile bur, with the grindings for each group of teeth being collected, weighed and stored frozen. The remaining half of the proximal surface of all teeth in each group was treated with one of the following agents: (1) saturated citric acid for 3 minutes; (2) 15% EDTA for 5 minutes; (3) sodium hypochlorite for 5 minutes, followed by a 30‐second application of 5% citric acid; (4) sodium hypochlorite alone for 5 minutes; and (5) 2% sodium deoxycholate for 1 minute, followed by a 1‐minute rinse in distilled water, and then a 1‐minute application of 5% Cohn's fraction IV1. The experimental areas were then removed with sterile burs and the grindings from each group were collected and weighed. The five control and five experimental grinding samples were treated by the phenol‐water extraction technique of Westphal and Jann and subjected to the Limulus Amebocyte Lysate test for determining the endotoxin content. Exposure to saturated citric acid or to sodium deoxycholate followed by Cohn's fraction IV1had little or no effect on endotoxin while use of EDTA resulted in only partial elimination of endotoxin. Sodium hypochlorite or sodium hypochlorite followed by citric acid neutralization resulted in endotoxin levels of less than 1 ng/gram of tooth material. Additional testing of the effect of citric acid using a standardE. coliendotoxin showed that the acid had no effect on endotoxin levels.

ISSN:1049-8885    

DOI:10.1902/jop.1983.54.2.77

出版商:Wiley    

年代:1983

数据来源: WILEY

摘要:

Controversy surrounds theimportance of keratinized gingiva in maintaining periodontal health. A well‐defined animal model system is necessary to evaluate longitudinally the role of keratinized gingiva when plaque control is inadequate or where dental procedures (restorative, prosthetic or orthodontic) alter the periodontal environment. Facial gingiva was excised from eight primary incisors in miniature swine. Contralateral teeth were used as controls. The experimental teeth exhibited mucogingival defects at 3 and 6 month observation periods. The secondary teeth erupting into the experimental regions also exhibited recession and chronic mucogingival defects. The marginal tissue in regions devoid of keratinized gingiva demonstrated clinical signs of inflammation. No progressive gingival recession was present. Excision of keratinized gingiva to produce mucogingival defects in swine provides a convenient model system for evaluating the effect of dental procedures on periodontal health where little or no keratinized gingiva is present.

ISSN:1049-8885    

DOI:10.1902/jop.1983.54.2.81

出版商:Wiley    

年代:1983

数据来源: WILEY

摘要:

Our current conceptof periodontal disease is that some mediators found in the gingival sulcus are able to penetrate the epithelial barrier and produce tissue destruction in the periodontium. Endotoxin, one such mediator, recently has been the subject of considerable study. The purpose of this investigation was to substantiate the role of endotoxin in the pathogenesis of periodontal disease by ascertaining its action on cellular and subcellular components. In order to accomplish this, cultured human gingival fibroblasts were exposed to media containing either 300μg/ml or 500μg/ml of endotoxin and untreated medium for a period of 24 hours. Cells were harvested and prepared for electron microscopy by conventional methods. Electron microscopic examination revealed alterations in mitochondria, Golgi apparatus, lysosomal‐like bodies, cytofilaments and plasma membrane. Relationship of these alterations to pathologic processes is discussed.

ISSN:1049-8885    

DOI:10.1902/jop.1983.54.2.86

出版商:Wiley    

年代:1983

数据来源: WILEY

摘要:

Epithelial andfibroblast cells from the human gingival sulcus area were cultivatedin vitro, and their sensitivity to the herpes simplex virus (HSV) was studied. Fibroblasts were sensitive to HSV infection and supported virus multiplication as evidenced by nuclear inclusion bodies and a cytopathogenic effect. Epithelial cells which were primarily devoid of HSV antigens were infected with HSV as demonstrated by positive immunofluorescent staining and damage to the cells at a later stage. Epithelial cells that were found to harbor HSV antigens upon removal from the patients prior to infectionin vitro, maintained these antigens throughout the period ofin vitrocultivation. The sensitivity of both epithelial cells and fibroblasts from the gingival sulcus area to HSV infectionin vitrois significant for the understanding of the role of these tissues as a primary site of infection and as a possible reservoir for the latent virus between recurrences.

ISSN:1049-8885    

DOI:10.1902/jop.1983.54.2.91

出版商:Wiley    

年代:1983

数据来源: WILEY

摘要:

the facial marginalalveolar bone of dogs was exposed with a full‐thickness flap and touched by an activated electrosurgery electrode for periods of 0, 1, 5 and 10 seconds. Microscopic results were evaluated after periods ranging from 0 hours to 28 days. Various electrode exposure times produced similar changes in bone and periodontal ligament. The extent of the destruction was greater than that found in surgical areas not exposed to electrosurgery; healing and remodeling phases were also delayed in the electrosurgical sites.

ISSN:1049-8885    

DOI:10.1902/jop.1983.54.2.96

出版商:Wiley    

年代:1983

数据来源: WILEY

摘要:

The effectsof liquid protein diet (LPD) on gingival collagen metabolism were evaluated in young rats. Experimental animals received a LPD, while pair‐fed and ad libitum groups received a 20% casein diet. Prior to sacrifice at days 23, 35 and 48 post‐partum, animals from each group were injected with C14‐proline. Gingival tissues from the palate were excised and analyzed for collagen content and collagen synthesis. Results of body‐weight changes at day 48 showed a 20% decrease in the LPD group, while pair‐fed andad libitumgroups increased 47% and 267%, respectively. Collagen content per gram of gingiva was not significantly different among the three groups. Gingival collagen synthesis, however, did show significant differences among the three groups. It was concluded that while LPD effects on the overall body condition were profound, LPD exerts minimal effect on gingival collagen metabolism in young rats.

ISSN:1049-8885    

DOI:10.1902/jop.1983.54.2.101

出版商:Wiley    

年代:1983

数据来源: WILEY

摘要:

Two casesof melanoacanthoma occurring on oral mucous membranes are presented. Melanoacanthoma is considered to be a benign mixed tumor of keratinocytes admixed with pigment‐laden dendritic melanocytes. All intraoral melanoacanthomas reported thus far have been pigmented lesions of the buccal mucosa and have occurred in young to middle‐aged black females. These tumors may grow rapidly and therefore they often mimic the radial growth phase of an intraoral melanoma. Because melanoacanthomas have no distinguishing clinical features, biopsy is mandatory.

ISSN:1049-8885    

DOI:10.1902/jop.1983.54.2.107

出版商:Wiley    

年代:1983

数据来源: WILEY

摘要:

A 22‐year‐oldblack male was referred for periodontal therapy because of radiographic evidence of advanced bone loss associated with the posterior teeth. Clinical examination revealed gingivitis, normal sulcus depths, and minimal loss of clinical attachment. Complete blood counts, serum chemistry, and neutrophil function were within normal limits. Histological, histochemical and ultrastructural analysis of an extracted tooth revealed no loss of attachment; large areas of the cementum were collagen‐poor and, ultrastructurally, resembled afibrillar cementum. It is proposed that the periodontal attachment mechanism present in this case was associated with a localized failure in normal periodontal development.

ISSN:1049-8885    

DOI:10.1902/jop.1983.54.2.112

出版商:Wiley    

年代:1983

数据来源: WILEY

ISSN:1049-8885    

DOI:10.1902/jop.1983.54.2.i

出版商:Wiley    

年代:1983

数据来源: WILEY