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1. |
Epidermal migration during the healing of suction blisters in rat skin: A scanning and transmission electron microscopic study |
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American Journal of Anatomy,
Volume 153,
Issue 2,
1978,
Page 177-191
Stephen C. Pang,
William H. Daniels,
Robert C. Buck,
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摘要:
AbstractFluid‐filled blisters, created on the skin of the inner surface of the pinna of heparinized rats by the application of suction, showed an intact basal lamina through which protruded hairs and remnants of hair follicles. The origin of epithelial cells and their spreading over the basal lamina were studied at intervals over 36 hours using scanning and transmission electron microscopy. The majority of epidermal cells arose from the outer root sheath of hair follicles and began to spread radially before 12 hours. By 36 hours the cells were confluent over most of the blister base. Inflammatory cells were few in number and rested mainly on the bare basal lamina rather than on the resurfacing cells. Some elongated epidermal cells showing tapering foot processes appeared to be migrating in from the epidermis at the edge of the blisters. The cells spreading from hair follicles were flat and polygonal. On these sheets of cells a few small folds and many filopodia were present at intercellular junctions and at the free margin of the leading cells. Large numbers of short microvilli covered the surface of some of the epidermal cells, especially at 24 and 36 hours; other epidermal cells were relatively smoot
ISSN:0002-9106
DOI:10.1002/aja.1001530202
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1978
数据来源: WILEY
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2. |
Cytology and distribution of secretory cell types in the mouse hypophysis as demonstrated with immunocytochemistry |
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American Journal of Anatomy,
Volume 153,
Issue 2,
1978,
Page 193-215
Burton L. Baker,
Douglas S. Gross,
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摘要:
AbstractSince differential chemical staining has been unsatisfactory for demonstration of specific secretory cell types in the hypophyseal pars distalis of the mouse, the objective of this study was to determine whether the peroxidase‐antiperoxidase immunocytochemical procedure might be more effective. Accordingly, representative sections from the hypophyses of 17 female and 15 male adult mice of the Swiss‐Webster strain were immunostained, 16 antisera to 5 pituitary hormones or their subunits being utilized. Five secretory cell types were demonstrated. Somatotropes were ovoid to spheroidal and distributed quite generally in the gland except for the “sex zone” where they were scarce. Somatotropes were larger and more numerous in the male than in the female. Mammotropes were polyhedral and also generally distributed in the gland except for the “sex zone” where few were observed. Mammotropes were larger and more numerous in the female than in the male. Corticotropes were small, stellate and few. They were most common near the ventral surface of the gland and formed bilateral centromedial groups in the lateral wings. Thyrotropes were usually large and polyhedral. They were restricted almost solely to the ventral region of the pars distalis. Gonadotropes were polyhedral, and generally distributed, except for aggregation in the cephalomedian “sex zone.” Most gonadotropes appeared to contain both luteinizing hormone and follicle‐stimulating hormone. Thus, all secretory cell types recognized in other species can be demonstrated readily in the mouse hypophysis with im
ISSN:0002-9106
DOI:10.1002/aja.1001530203
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1978
数据来源: WILEY
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3. |
Ultrastructural identification of afferent fibers of cardiac origin in thoracic sympathetic nerves in the dog |
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American Journal of Anatomy,
Volume 153,
Issue 2,
1978,
Page 217-231
Jeanne L. Seagard,
Harlan J. Pederson,
David R. Kostreva,
Diane L. Van Horn,
Joseph F. Cusick,
John P. Kampine,
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摘要:
AbstractWhile cardiac afferent nerve activity has been recorded from the ventrolateral (VLCN) and ventromedial (VMCN) cardiac nerves, left dorsal and ventral ansae subclaviae, and left upper thoracic white rami communicantes, little anatomical evidence for the existence of afferent fibers in these nerves has been reported. This study was designed to characterize the normal ultrastructure of the above nerves and to identify afferent fibers in them through Wallerian degeneration produced by dorsal root ganglionectomy. Laminectomies followed by dorsal root ganglionectomies were performed on left thoracic roots T1‐T4in six mongrel dogs. The nerves to be examined were removed from two animals at 1, 2, and 3 weeks following ganglionectomy and prepared for electron microscopy. Control nerves were obtained from two normal dogs. Degenerating nonmyelinated fibers were characterized by watery axoplasm containing clumps of electron‐dense material. Degenerating myelinated fibers were distinguished by the separation of their myelin lamellae, producing characteristic whorls. After three weeks, afferent nonmyelinated axons had degenerated in all nerves, leaving only layered processes of Schwann cells in these areas. Approximately 5–15% of the fibers in each nerve degenerated, indicating their afferent nature. Of these fibers, 85–90% were nonmyelinated C fibers and the remainder myelinated Aδ fibers. These results indicate participation of both Aδ fibers and a large population of C fibers in transmission of cardiac afferent
ISSN:0002-9106
DOI:10.1002/aja.1001530204
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1978
数据来源: WILEY
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4. |
Localization of vitamin A‐storing cells in the mouse adrenal gland: An electron microscopic autoradiographic study |
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American Journal of Anatomy,
Volume 153,
Issue 2,
1978,
Page 233-249
Kazushige Hirosawa,
Eichi Yamada,
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摘要:
AbstractRadioactive vitamin A acetate was stored in specific cells in the mouse adrenal gland. Radioactivity was detected in lipid droplets of fibroblast‐like cells, whose distribution was from the capsule to the medulla. Their population was highest in the cortex. The morphology of these labeled cells was similar to that of vitamin A‐storing cells in other organs of mouse (Yamada and Hirosawa, 1976).Though functions of these cells are obscure, the fact that they are found in organs where metabolic activity of vitamin A is high suggests that they bear some role in the metabolism and storage of the vita
ISSN:0002-9106
DOI:10.1002/aja.1001530205
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1978
数据来源: WILEY
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5. |
Structure of the chicken's inner ear: SEM and TEM study |
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American Journal of Anatomy,
Volume 153,
Issue 2,
1978,
Page 251-271
Katsuhiko Tanaka,
Catherine A. Smith,
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摘要:
AbstractThe inner ears of 35 adult chickens were studied by TEM, SEM and light microscopy. Two well differentiated hair cell/nerve ending units were present: tall hair cells with small vesiculated nerve endings were located on the attached part of the basilar membrane; short hair cells with large vesiculated nerve endings were located on the free basilar membrane except for the distal tip. In this respect the chicken ear is similar to that of the pigeon. The chickens examined did have some unique features. Sensory cells of lenticular and hemispheric shape were also present at the proximal end. Bundles of long dense tubules were seen frequently within the sensory cell cytoplasm. Kinocilia were absent from the hair bundles of many of the sensory cells. The internal structure of the kinocilia which were present was atypical and consisted of a variable number of doublets. Eight peripheral plus one central doublet were found most frequently.
ISSN:0002-9106
DOI:10.1002/aja.1001530206
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1978
数据来源: WILEY
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6. |
The fine structure of the ventricular surface of the area postrema of the cat, with particular reference to supraependymal structures |
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American Journal of Anatomy,
Volume 153,
Issue 2,
1978,
Page 273-290
R. A. Leslie,
D. G. Gwyn,
C. M. Morrison,
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摘要:
AbstractThe ependymal surface of the feline area postrema (AP) has been examined with the scanning and transmission electron microscopes. Particular attention was paid to the distribution and structure of supraependymal (SE) cells which have not been described before on this organ. A population of SE cells was found consistently on the cat AP; they were grouped most frequently toward the caudal aspect of the organ. Their structure bears a great resemblance to that of macrophages, containing: (1) many small apical vacuoles, (2) numerous lysosomes, and (3) phagosome‐like bodies. Arrays of microvillous tufts and also singly‐occurring microvilli are features of the unciliated ependymal surface of the organ. Tufts are often found in very close proximity to superficial blood vessels which are characteristically surrounded by large perivascular spa
ISSN:0002-9106
DOI:10.1002/aja.1001530207
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1978
数据来源: WILEY
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7. |
Scanning electron microscopy of the kidney glomerular epithelium after treatment with polycations in situ and in vitro |
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American Journal of Anatomy,
Volume 153,
Issue 2,
1978,
Page 291-303
Peter M. Andrews,
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摘要:
AbstractChanges in fine structure of glomerular epithelial cells (podocytes) were examined after treatment in situ and in vitro with polycations (i.e., protamine sulfate and poly‐l‐lysine). Scanning and transmission electron microscopic observations indicate that polycation‐induced alterations of the glomerular epithelium are similar in many respects to those resulting from puromycin aminonucleoside nephrosis. These alterations include the formation of junctional complexes between adjacent podocyte processes, flattening and retraction of podocyte processes, extensive folding of the slit diaphragms, formation of interpedicular microbridges, and development of irregular knobby surface‐microprojections. Prolonged treatment in vitro with high concentrations of polycations results in nearly complete loss of podocyte processes and the transformation of these once elaborate cells into simple polyhedral cells. The foregoing observations support the view that pedicel loss in puromycin aminonucleoside nephrosis may be due to a reduction in the glomerular epithelial polyanionic sialic acid surfa
ISSN:0002-9106
DOI:10.1002/aja.1001530208
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1978
数据来源: WILEY
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8. |
The role of lymphoid cells in bone resorption: Cellular immunological competence iniarats |
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American Journal of Anatomy,
Volume 153,
Issue 2,
1978,
Page 305-319
Gary B. Schneider,
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摘要:
AbstractTheia(incisors absent) rat is a naturally occurring mutant that has osteopetrosis resulting from reduced bone resorption. A number of in vestigators have reported that other osteopetrotic mutant rats (op) and mice (miandop), in addition to demonstrating the skeletal manifestations of the disease, have cellular immunological defects. In light of the fact that bone resorption has been demonstrated to be controlled by hematopoietic tissues and more specifically by mononuclear cells isolated from lymphoid organs, it seemed appropriate to investigate immunological competence in theiarat, to determine whether or not it had this defect in common with theoprat.Contrary to the results presented for other osteopetrotic mutants, we found that the thymuses in ouriamutants had a normal cellular composition and the T‐cell‐dependent zones in the lymph nodes were not deficient in lymphocytes. Therefore, this study was undertaken to investigate the response ofiarats to oxazolone, which produces one type of T‐cell‐dependent immune response, delayed hypersensitivity.The results of these studies indicate thatiarats at 18 days of age have the ability to develop delayed hypersensitivity, as shown by both a blastogenic response in the lymph node draining the site of sensitization and a positive skintest. The magnitudes of these responses were similar iniaand normal littermates, both histologically and quantitatively. Therefore, theiamutant does not appear to have a defect of the thymus‐dependent immune system, and further studies should be carried out to investigate other parameters of immunological competence in thes
ISSN:0002-9106
DOI:10.1002/aja.1001530209
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1978
数据来源: WILEY
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9. |
Masthead |
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American Journal of Anatomy,
Volume 153,
Issue 2,
1978,
Page -
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PDF (40KB)
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ISSN:0002-9106
DOI:10.1002/aja.1001530201
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1978
数据来源: WILEY
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