摘要:

AbstractThe numerous compound acinar, mixed, bronchial glands of the opossum's lungs collectively provide a large surface for secretion. The secretory materials are made by two types of cells located in the acini and smaller ducts. Mucus is produced by mucous cells that are similar to those found elsewhere. A fluid of unknown composition appears to be secreted by hydrotic cells. The acini are encircled by myoepithelium and the larger ducts are lined by morphologically unspecialized cells. The hydrotic cell, a type of “special serous cell,” is cytologically the most interesting of the elements present. It is not appreciably basophilic and lacks secretion granules, but it is rich in glycogen and contains many mitochondria with high succinoxidase activity. The basal surface is divided into irregular compartments by grooves, some of which are occupied by processes from stellate myoepithelial cells. The apical surface is villous and extends far down the sides of the cell as intercellular canaliculi. This surface has adenosine triphosphatase activity and is contacted by the agranular reticulum and numerous small vesicles in the subjacent cytoplasm. The bronchial glands are classified in the discussion, and special features of the hydrotic cells are compared with those possessed by other types of cells that engage in ion‐segregation or ion‐secretion. The glands are thought to assist in temperature reg

ISSN:0002-9106    

DOI:10.1002/aja.1001170302

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1965

数据来源: WILEY

摘要:

AbstractThe cytology and cytochemistry of the pitiutary gland of the palm squirrelFunambulus pennantiduring different phases of reproductive activity are described. Two types each of basophils and acidophils are recognized. The basophils include gonadotrophs and thyrotrophs and acidophils include orangeopils and carminophils. The gonadotrophs which are ovoid, coarsely granulated and mainly located on the sinusoidal borders of the lateral halves of the pars anterior are more numerous in males than in females. They are PAS‐positive and AF‐negative. They cannot be distinguished tinctorially into FSH and LH secreting cells as in the pituitary of the rat. The thyrotrophs which are large, rounded or polyhedral, finely granulated cells are confined to the antero‐median zone of the pars anterior. They are PAS and AF‐positive. The basophils can be tinctorially differentiated by the performic acid‐alcian blue‐PAS‐orange G staining in which the gonadotrophs are stained purple and the thyrotrophs are red. The acidophils are differentiated tinctorially into two types. The orangeophils or STH secreting cells are small, rounded, and coarsely granulated. Carminophils or LTH secreting cells which appear in the pituitaries of females in early pregnancy and increase in number during later pregnancy and lactation, are large and coarsely granulated. The acidophils are specifically stained with mercury‐

ISSN:0002-9106    

DOI:10.1002/aja.1001170303

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1965

数据来源: WILEY

摘要:

AbstractChanges in the cytology of the pituitary gland of the palm squirrel,Funambulus pennanti(Wroughton), following gonadectomy, androgen administration and propylthiouracil treatment are described.Increase in gonadotroph cell number and granulation reaches its maximum by one week after gonad ablation and is maintained at this level during the next three weeks, following which there is a gradual decline. Female squirrels show hypertrophied cells with prominent Golgi apparatus at 16 weeks after gonadectomy whereas males show similar changes four weeks after castration, changes which are still clearly noticeable at the end of 48 weeks. Castration cells or “signet‐ring cells,” so characteristically seen in rats, do not occur in gonadectomized squirrels. Thyrotrophs are not affected by gonadectomy. Acidophils quickly decrease in number following gonadectomy, and are maintained at this level throughout the post‐castration period.Administration of testosterone propionate to one week castrates causes degranulation in some gonadotrophs while others are coarsely granulated and deeply stained. The degranulated cells are interspersed with the other deeply stained gonadotrophs in the lateral halves of the pars anterior. Testosterone propionate administration to 12‐week castrates produces no marked changes in the granule content of the gonadotrophs.Propylthiouracil treatment causes degranulation of cells located in the anteromedian zone of the pars anterior. Degranulation is partial in animals treated for 15 days but is compete after 30 days of treatment. Acidophils are partially degranulated after 30 days of propylthiouracil

ISSN:0002-9106    

DOI:10.1002/aja.1001170304

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1965

数据来源: WILEY

摘要:

AbstractPaneth cells from fasted, fed and pilocarpine stimulated mice were studied with the light and electron microscopes. While human and rat Paneth cells contain structurally homogeneous granules, most mouse Paneth cell granules were found to be structurally heterogeneous both by light and electron microscopy. These structurally heterogeneous granules consisted of an outer rim or halo which surrounded the central core of the granule. These halos varied strikingly in electron density ranging from much paler to much darker than the granule core. Electron density of the halos correlated well with their affinity for toluidine blue in light microscopic preparations. The granule halo could be stained with both alcian blue and with PAS indicating that it contained acid mucopolysaccharide while the central core of the granule could be stained only with PAS and not with alcian blue.While actively secreting Paneth cells were seen in preparations from fasting mice, the secretory activity was increased after feeding and after stimulation with pilocarpine. The actively secreting mouse Paneth cells discharged their granules into the crypt lumen by merocrine secretion. The fine structure of mouse Paneth cell granules was the same in resting and in actively secreting cells.

ISSN:0002-9106    

DOI:10.1002/aja.1001170305

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1965

数据来源: WILEY

摘要:

AbstractWith electron microscopy it was observed that ferritin antigen was taken up in pinocytotic vesicles by the reticular cells. No antigen was observed in lymphocytes, in immature or mature plasma cells after primary and secondary stimulation, or after hyperstimulation. In hyperstimulated tissue a larger number of more complex vesicles containing ferritin were visible, more ferritin was found free in the cytoplasm and a larger number of multiple‐membraned figures were present.Hyperstimulation led to the appearance of activated fixed reticular cells which were characterized by cytoplasm containing a rich endoplasmic reticulum with dilated cisternae and ferritin both free and in vesicles. The activated fixed reticular cells therefore contain antigenic ferritin and have the characteristics of cells engaged in protein synthesis.Using fluorescent antibody staining no antigenic material was detectable in the antibody producing plasma cell; therefore if antigen fragments not identifiable in electron microscopy are present they are in a form not available for antibody staining. Gamma‐globulin was not detectable in the activated reticular cell. It may be necessary for the intact antigen to be completely modified and for the cell to lose its fixed position before γ‐globulin synthesis can

ISSN:0002-9106    

DOI:10.1002/aja.1001170306

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1965

数据来源: WILEY

摘要:

AbstractPrior studies have established a definite interrelationship between the salivary glands and the endocrine system. This study was designed to histochemically characterize testosterone‐induced changes in the submandibular and the sublingual glands of male and female Ajax strain mice. In addition to morphologic stains, histochemical procedures included the demonstration of tryptophan, tyrosine, and sulfhydryl groups. Alcian blue, PAS, and toluidine blue staining was also employed. The Gomori and azo dye coupling procedures were both utilized for the demonstration of alkaline phosphatas

ISSN:0002-9106    

DOI:10.1002/aja.1001170307

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1965

数据来源: WILEY

摘要:

AbstractThe presence of relaxin in uteri, placentae, and ovaries of pregnant rats was studied immunohistologically with fluorescein labeled anti‐relaxin serum and confirmed by histological and histochemical techniques.In sections of uteri from day 12 of pregnancy stained with these antisera, the cytoplasm of the granular cells of the mesometrial decidua gave off a yellow‐green fluorescence indicating antirelaxin binding. On day 15, 17 and 19, the granular cells of the metrial gland and occasional lakes in near‐by vascular lumina fluoresced. No other specific fluorescence was evident in the uteri or placentae. The fluorescent regions of these cells corresponded exactly with the cytoplasmic aggregates of granules in the metrial gland and mesometrial decidua. Histochemical studies of these granules which bound with antirelaxin, revealed a chemical composition congruent with that known for relaxin, and with that of the relaxin‐containing granules of human decidual and placental cells. The dissolution of connective tissue fibers, believed to be a main function of relaxin, was observed in the last days of pregnancy within the metrial gland, especially where it borders the mesometrial decidua. These results correlate well with Schaub's discovery of a collagen‐degrading substance in the metrial gland. Other actions of relaxin may be explained as secondary to the primary effect of dissolution of collagen. The relaxin‐containing cells in the human and in the rat differ only in their localization in the tissue which depends on their phylogenetic adaptation to different placentational needs. The metrial gland cells of the pregnant rat correspond in their function to the basal trophoblastic cells of the human term placenta. The non‐menstruating rat has no counterpart for the endometrial granulocytes, but the granular cells in the mesometrial decidua adjacent to the site of implantation correspond to the endometrial granular cells surrounding the implantation site in the human; it is suggested their collagen dissolving action facilitates

ISSN:0002-9106    

DOI:10.1002/aja.1001170308

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1965

数据来源: WILEY

ISSN:0002-9106    

DOI:10.1002/aja.1001170301

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1965

数据来源: WILEY