摘要:

656 skin biopsies with positive direct immunofluorescence from the UK and overseas were studied over a 2-year period. The length of time biopsies had remained in Michel’s medium at pH 7.0 in various diseases (pemphigoid, pemphigus, linear IgA disease, epidermolysis bullosa acquisita, lupus erythematosus, vasculitis, amyloid, lichen planus and dermatitis herpetiformis) was analysed. We concluded that direct immunofluorescence remained positive at 6 months and that Michel’s medium is a reliable long-term maintenance medium for skin biops

ISSN:1018-8665    

DOI:10.1159/000246955

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

We compare a series of conditions for inducing lamina lucida separation through normal skin specimens using 1 M NaCl for the detection of basement membrane zone auto-antibodies using indirect immunofluorescence. Skin was split at room temperature and at 4 °C with and without phenylmethylsulphonyl fluoride. Our results demonstrate that for the detection of bullous pemphigoid and epidermal-binding linear IgA bullous dermatoses antibodies skin should be split at 4°C with enzyme inhibitors. For epidermolysis bullosa acquisita and dermal-binding antibodies skin can be split at room temperatur

ISSN:1018-8665    

DOI:10.1159/000246956

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

In 18 patients with dermatitis herpetiformis (DH) of whom 16 were HLA-DR3 positive and 14 had the HLA-B8, -DR3 haplotype, the frequencies of known mutations of the tumor necrosis factor α (TNF-α) and TNF-β genes were investigated using restriction fragment length polymorphism analysis and the single-strand conformational polymorphism technique. In DH, the phenotype frequency (28%) and allele frequency (0.58) of the rare TNF-β allele TNFB*1 were significantly increased (normal control 12.4% and 0.37). For the TNF-α promoter/enhancer polymorphism, the rare allele TNF2 was more frequent in DH patients (11%, 0.47) compared to controls (2%, 0.16). Since functional studies have associated the rare TNFB*1 and TNF2 alleles with a higher secretion of TNF upon activation in vitro, the predominance of these two ‘high-response’ TNF alleles in DH patients may represent a genetic basis for the chronic inflammatory response in the skin and mucosal tissues of patients

ISSN:1018-8665    

DOI:10.1159/000246957

出版商:S. Karger AG    

年代:1994

数据来源: Karger

ISSN:1018-8665    

DOI:10.1159/000246958

出版商:S. Karger AG    

年代:1994

数据来源: Karger

ISSN:1018-8665    

DOI:10.1159/000246959

出版商:S. Karger AG    

年代:1994

数据来源: Karger

ISSN:1018-8665    

DOI:10.1159/000246919

出版商:S. Karger AG    

年代:1994

数据来源: Karger