摘要:

In a retrospective and prospective analysis fluorescence microscopy of Papanicolaou stained bronchoalveolar lavage specimens has been applied to the diagnosis ofPneumocystis carinii(PC) in routine cytology. the pneumocysts presented as circular structures of 5 μm in diameter and of brilliant green‐yellow fluorescence surrounding two mirror image reniform structures. Fluorescent inclusions of 1–3 μm diameter within the alveolar macrophages could be identified as remnants of pneumocysts by a follow‐up of all steps of degradation ending in very small irregular granules. By applying both criteria, i.e. pneumocysts with reniform bodies and degradation inclusions within macrophages,Pneumocystis cariniipneumonitis (PCP) could be detected in 100% of cases. Transbronchial biopsy permitted the correct diagnosis in only 65.2% of cases. Retrospective analysis of slides is possible after a long period as no significant loss of fluorescence occurs after 4 years. Thus fluorescence microscopy permits the diagnosis ofPneumocystis cariniiwithout any additional staining or loss

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1991.tb00395.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Papanicolaou stained smears of urinary sediment containing inclusion bearing urothelial cells suggestive of human polyomavirus infection were destained and reprocessed forin situhybridization using a biotinylated probe for human polyomavirus DNA. Seven slides were processed in this way. A hybridization signal for viral DNA was noted in each case, even in smears that had previously been stored for 11 years. This simple and rapid non‐radioactive detection system is a valuable supplement to routine urinary cytology for the definitive diagnosis of this virus infectio

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1991.tb00396.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

A retrospective cytological study of nine follicular tumours of the thyroid with clear cell change was undertaken. In five clear cell adenomas and one moderately differentiated clear cell follicular carcinoma the epithelial cells occurred singly or in sheets and clusters; they sometimes assumed a trabecular or follicular pattern. the cells usually had pale diffusely vacuolated cytoplasm with ill‐defined boundaries, a variable degree of anisonucleosis, nucleolar enlargement, and nuclear overlapping. Smears from a signet‐ring cell adenoma contained in addition a few cells with large cytoplasmic vacuoles and compressed eccentric nuclei.In these cases a cytological diagnosis of ‘follicular lesion’ (or follicular neoplasia), clear cell type or signet‐ring cell type, was given. A cytodiagnosis of ‘carcinoma’ was made only in the poorly differentiated follicular carcinoma‐clear cell variant studied which showed unequivocal features of malignancy. Features suggestive of thyroid cyst, nodular goitre, Hashimoto's thyroiditis, and cell hyperactivity (marginal vacuoles, ‘fire flare’) were also found in the aspirated specimens of these cases of clear cell tu

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1991.tb00397.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

The cytological and immunological findings of 81 metastatic melanomas are described. Fine needle aspiration was performed from secondary deposits in lymph nodes (38), subcutaneous and soft tissue (36), abdomen (5), lung (1) from 67 patients with histologically verified malignant melanoma. One patient had disease which had spread into the cerebrospinal fluid. Cytomorphologically the cases were classified as classical (47%), carcinoma‐like (22%), spindle cell type (14%), lymphoma like (6%), undifferentiated (6%), myxoid type (3%), and clear cell type (2%). All cases were immunologically characterized using antibodies to S‐100, vimentin and cytokeratin. All cases were S‐100 positive and the majority (96%) reacted with antibodies to vimentin. A weak heterogenous reactivity to cytokeratin antibody was detected in only eight cases. the HMB45 antibody was applied to 20 cases and 16 (80%) of these tumours were positive. In summary, we found that an immunological characterization was necessary to conclusively diagnose over 50% of metastatic melanomas which presented with an equivocal cytological pi

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1991.tb00398.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

The cytomorphology of 13 cases of Langerhans cell histiocytosis is described. the most striking features were the presence of intranuclear clefts, pale nuclei and inconspicuous nucleoli, together with ample pale cytoplasm, only slight cellular pleomorphism, and an admixture of varying numbers of eosinophils, macrophages and degenerated cells.In 13 of 16 cases investigated ultrastructurally, characteristic Birbeck granules were detected. Out of six cases tested, four exhibited positivity for S‐100, and of three cases tested, all were positive for CD1a (leu 6) and HLA‐DR. In one case malignant transformation occurred, terminating in monocytic leukae

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1991.tb00399.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

We present a case of a 45‐year‐old woman with a benign breast lump in which collagenous spherulosis was an incidental finding. Since collagenous spherulosis has a similar appearance to adenoid cystic carcinoma of breast on fine needle aspiration cytology, the two conditions can easily be confused. the problem of making this important distinction is discus

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1991.tb00400.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1991.tb00401.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY