ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1990.tb00328.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

This paper describes a study of cervical screening in 50 general practices in the East Berkshire Health District over a period of 2½ years. Six practices organized their own practice based screening scheme. Thirty‐nine participated in a district organized scheme and five did not actively participate in any scheme.The proportion of women screened was highest among the practices which organized their own scheme. These practices were large, in non‐urban locations and employed a practice nurse. In contrast, practices which had a poor record of screening were small, single handed, in an urban location and were unwilling to participate in a district call scheme or organize their own scheme.It is recommended that the practices which are prepared to organize their own screening programmes should be encouraged to do so. The resources which are saved could then be more usefully spent on providing assistance to the practices which do not offer a cervical screening service to women on their practice l

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1990.tb00329.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Solid renal masses in 120 patients were assessed by percutaneous fine needle aspiration cytology, performed under radiographic guidance. Follow‐up was obtained in all but seven cases. Diagnostic sensitivity for 83 tumours in the series was 91.6%, while two false positive reports resulted in a specificity of 93.8%. Positive and negative predictive values were 97.4% and 81.1% respectively. One advantage of the procedure was the ability to cell type neoplasms accurately, which enabled administration of pre‐operative chemotherapy in childhood tumours, and selection of appropriate surgical procedures in adults. The technique offered a non‐surgical means of confirming primary renal tumours in patients presenting initially with distant metastases, and documenting renal secondaries in cases with malignancy at other sites. The diagnosis of benign or inflammatory lesions permitted institution of definitive th

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1990.tb00330.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A study of the cytological appearances of benign and malignant colorectal adenomatous polyps is reported. The aim of the study was to characterize the cytological features of adenomatous polyps and predict the likelihood of malignancy using cytology. A five grade classification of colorectal cytology has been developed and the characteristic appearances of cells from adenomatous polyps are described. The reproducibility of cytological diagnosis based on this classification has been tested in 120 smears from normal mucosa and adenomatous polyps (including polyp cancers). Correlation with histology was achieved in 88% and correlation of the cytological diagnosis between two observers was achieved in 84%. We conclude that cytology can be used reliably as an adjunct to histology in the assessment of malignancy of adenomatous polyps.

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1990.tb00331.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Fine needle aspiration of the male breast can present problems of diagnosis because the cytological presentation of gynaecomastia can be confused with that of adenocarcinoma. We reviewed breast aspirates from 24 male patients in order to determine the accuracy of cytology as a method of diagnosing gynaecomastia.Discrepancies were observed between the original cytology reports on one hand and the review cytology and biopsies on the other. Of the 24 aspirates from the male breast, the cytology was reported as negative in 16 cases, suspicious in three cases and malignant in five. In four cases of the negative group, a specific diagnosis of gynaecomastia was made. In two of the negative cases the subsequent biopsies revealed adenocarcinoma. Of the five cases reported on the original cytology as adenocarcinoma, two on review showed the features of florid gynaecomastia and this was confirmed on biopsy and three confirmed the initial diagnosis of adenocarcinoma.The cytological features of gynaecomastia which distinguish it from adenocarcinoma are discussed.

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1990.tb00332.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The extraction of DNA from archival exfoliative cytology samples would allow the molecular biological analysis of this readily available material using the polymerase chain reaction (PCR). We have quantitatively and qualitatively studied the extraction of DNA from a variety of cytological preparations.For both fresh and archival cervical smears, overnight incubation with proteinase K produces high yields of high molecular weight DNA, but simply boiling the samples produces DNA suitable for PCR amplification of a single copy gene.Increasing the proteinase K incubation to several days allows the extraction of DNA from fixed and stained archival cytology slides from a variety of sites. The extracted DNA was again suitable for PCR analysis.Fresh and archival cytological material can be utilized for molecular biological study of disease processes using PCR. Archival cytological material is probably the best source of DNA and RNA after stored frozen tissue.

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1990.tb00333.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A series of 51 genital biopsies from normal epithelium, condylomata acuminata, leucoplakia and squamous cell carcinoma from Chinese male and female patients were analysed for the presence of human papillomavirus (HPV) types 6, 11, 16 and 18 by DNA in situ hybridization. All of the nine genital condyloma acuminata were positive for HPV DNA, in which HPV 6 was found in six cases, HPV 11 in two cases and HPV 18 in one case. Twelve out of the 21 cases (57.1% of the total) of cervical squamous cell carcinoma were shown to contain HPV DNA; HPV 16 was found in nine cases, HPV 18 in two cases and HPV 16/18 in one case. Present results support the earlier concept that HPV 6/11 are closely associated with benign genital lesions, and HPV 16/18 are mostly confined to higher grade of intra‐epithelial neoplasias and carcinom

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1990.tb00334.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Using a system of immunocolloidal gold labelling, we have monitored the expression and distribution of transferrin receptors (TfRs) within the K562 cell line, during NK/target cell interactions. An indirect method of immunolabelling was used to effectively immunolabel tumour cells without disrupting the natural effectontarget interactions. Successful localization of TfRs demonstrates the potential of the described technique for discerning antigenic distribution of other cell:cell interactions.Immunolabelling has also provided a useful method for demonstrating receptor down‐regulation within NK target cells, as a proposed cause of reduced receptor expression by TPA‐treated cells. Following 30 and 60 min incubation periods with TPA, approximately 15 and 30%, respectively, of the gold/antibody complexes were relocated from the surface membrane to an intracellular location within endocytotic vesicles. The demonstration of receptor down‐regulation is important as a proposed cause of TPA‐induced tumour cell resistance to NK‐mediated

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1990.tb00335.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

In order to assess the value of immunocytochemical staining as a method of discriminating between benign reactive mesothelial cells and malignant epithelial cells in serous effusions, we have studied the reactions of a panel of commercially available antibodies on cells harvested from 83 pleural and peritoneal fluids and compared the results with the clinical and cytological diagnoses. The antibodies used were raised against cytokeratin (PKK1), epithelial membrane antigen (EMA), carcino‐embryonic antigen (CEA), pregnancy specific B1‐glycoprotein (SP1) and leucocyte common antigen (LCA). Anti‐CEA was positive in 16 of 39 effusions (41%) containing carcinoma cells. Pregnancy specific Bl‐glycoprotein (SP1) was positive in 33% of the same samples. Mesothelial cells did not stain with these antibodies. Thus anti‐CEA and SP1 can be used to discriminate between benign mesothelial and malignant epithelial cells in effusions. Anti‐PKK1 stained both benign reactive mesothelial cells and malignant epithelial cells and cannot be used to discriminate between these two cell types. Strong positive staining of malignant cells was noted with anti‐EMA. However, as occasional weak staining of mesothelial cells was also noted, strong staining with this antibody may be regarded as suspicious but not conclusive

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1990.tb00336.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1990.tb00337.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY