ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1995.tb00472.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Thebcl‐2protein plays a role in the regulation of programmed cell death (PCD), overriding apoptosis. Its expression has been reported in breast ductal cells, where it is believed to be involved in the hormonal regulation of hyperplasia and involution. to date,bcl‐2gene product has not been investigated on breast cancer FNA. the expression ofbcl‐2protein was evaluated using an immunoalkaline phosphatase technique in 54 pre‐operative breast cancer aspirates and in paraffin‐embedded sections from 20 matched surgical specimens. A high rate ofbcl‐2protein expression was found on FNA samples (65%) and on the corresponding tissue sections (60%); there was a nearly absolute concordance in the two specimens, with 19/20 (95%) cases showing a concordant staining. These findings concur with the view thatbcl‐2gene is frequently expressed in breast cancer, possibly through a hormonal‐de

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1995.tb00473.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

A 45‐year‐old male presented with a large mass in the left axilla. FNA cytology was interpreted as Hodgkin's disease (HD), lymphocyte depletion (LD) type, but histopathologic and immunohistochemical examination showed features of Ki‐1‐positive anaplastic large cell lymphoma. Unrepresentative sampling by the FNA from the tumour periphery resulted in a false impression of dual reactive and neoplastic cell populations. which together with the frequent Reed‐Sternberg‐like cells led to the initial erroneous impression of HD. Therefore, the cytologic diagnosis of HD, LD should be approached w

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1995.tb00474.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

FNA plays an important role in preoperative diagnosis of soft tissue tumours. A close clinical/morphologic cooperation is essential. FNA should be performed on the most accessible part of the tumour, avoiding penetration of the deep portions of the tumour. Needles 0.7 mm (22 G) are recommended. For deep lesions, needles with a stylet should be used. After the FNA, tattooing of the aspiration channel is recommended, and the channel is surgically removed together with the tumour, if a sarcoma. Material from the FNA can be used for additional examinations, i.e. electron microscopy, immunohistochemistry, DNA ploidy analysis and chromosomal analysis. Those techniques are of great importance in the differential diagnosis, particularly in the paediatric small/round cell tumours. the majority of sarcomas can be defined as low grade or high grade malignant in FNA. For malignancy grading the following parameters are used: cellularity, pleomorphism, chromatin pattern, nucleolar structure, mitotic figures and necroses. Cytodiagnostic details of the most common soft tissue tumours and their differential diagnoses are presented.

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1995.tb00475.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Two cases of necrosis of thyroid oxyphilic tumours following FNA are reported. the first patient received surgery 4 weeks after FNA and histological examination revealed an encapsulated and totally necrotic tumour 2 cm in diameter. In the second patient surgery was performed after 25 days. Histological examination showed a 0.7 cm diameter tumour consisting mainly of fibrous tissue with residual oxyphilic tumour cells only in a small peripheral rim. In both patients no capsular or vascular invasion and no blood vessel thrombosis were present. A review of the literature revealed that oxyphilic tumours are susceptible to post‐FNA necrosis, which might be due to the compromised vascular supply after FNA in conjunction with the intrinsic energy deficiency of oncocytic tumour cell

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1995.tb00476.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Adult rat hepatocytes were cultured for 15 days on type I collagen‐coated permeable membranes in a hormonally defined Waxman's modified medium supplemented with very low concentrations of insulin, glucagon and dexamethasone. Phase contrast examination showed that 15‐day‐old cultures still formed a regular monolayer of polygonal cells. In similarly aged cultures, intracellular glycogen was abundant and evenly distributed, while steatosis remained very limited. Scanning and transmission electron microscopy showed that well developed bile canaliculi could be observed on the lateral side of the hepatocyte membrane after 4 days of incubation and persisted for 2 weeks. These canalicular structures probably originated from coalescence of membrane invaginations observed in 1‐day‐old cultures. Transmission electron microscopy showed that the ultrastructure of the cells was very close to that of normal rat hepatocytes in the intact liver. These results suggest that rat hepatocytes cultured under these experimental conditions are able to develop and maintain tissue‐specific cytochemical and morphological properties for at le

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1995.tb00477.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Cytological examination of urine from the ileal conduit in cases of bladder cancer treated by radical surgery can be an important and effective follow‐up procedure. A total of 19 patients (18 males and one female) on whom radical cystectomy for cancer was performed were studied. Three urine specimens were examined in each case using routine cytological methods. Three cases of recurrent carcinoma (mainly of papillary type) were diagnosed cytologically before any clinical evidence of disease. the cytological examination of urine at 3‐6 month intervals after cystectomy for bladder carcinoma is considered advisable in all cases, since the recurrence rate of transitional cell neoplasms in the upper urinary tract after cystectomy for transitional carcinoma is quite h

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1995.tb00478.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1995.tb00479.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1995.tb00480.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1995.tb00481.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY