ISSN:0743-5800    

DOI:10.3109/07435809409030402

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

A sensitive and specific radioimmunoassay was used to measure basal oxytocin (OT) concentrations in samples of peripheral blood drawn q 10 min for 8 hrs from women during the follicular and luteal phases of the menstrual cycle and from men before and after 20 hrs of fasting. Few, if any, pulses of OT were found in the blood of men and women in this study. The pattern of OT concentrations in the circulation was the same during both stages of the menstrual cycle and was not affected by short-term fasting. The use of frequent sampling and the present assay system for OT did not detect a pulsatile pattern of OT concentrations in the blood of men and women in the basal state.

ISSN:0743-5800    

DOI:10.3109/07435809409030403

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

The cdc2 kinase family is known to be one of the important factors for cell proliferation in both yeast and mammalian cells. By using polyclonal antibodies against PSTAIRE region of cdc2HS, we studied the amount of cdc2-related kinases in the rat thyroid cell line, FRTL-5, during the cell cycle. The immunoreactive protein with molecular weight 34kD was hardly detectable, instead, 40kD protein exists constantly in amount through G0phase to S phase. Further, it did not decrease when the cells were cultured in serum- and hormone-free medium. Moreover, we observed an increase of this protein in the nuclear fraction as the cells enter S phase. On the contrary, we could not detect any immunoreactive 40kD protein in primary cultures of rat thyroid cells. These results may indicate abundant existence of this protein might concern cell cycle regulation of FRTL-5 or its immortal feature.

ISSN:0743-5800    

DOI:10.3109/07435809409030404

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

The effect of single and chronic ACTH administration on serum levels of the corticosteroid-binding globulin (CBG) and pituitary-adrenal (PA) responsiveness to acute immobilization (IMO) stress was studied in adult Sprague-Dawley rats. Single ACTH administration significantly reduced CBG levels but did not alter PA response to acute IMO. Chronic ACTH administration caused a greater fall in CBG than single ACTH administration and blunted PA response to IMO. The effect of chronic ACTH administration on CGB levels recovered 2 days after the last administration, but the ACTH response to IMO was normal only by day 7 after the last ACTH injection. The present data indicate that ACTH administration to rats reduced CBG levels and impaired PA response to acute stress, but impaired PA responsiveness cannot be solely attributed to changes in CBG.

ISSN:0743-5800    

DOI:10.3109/07435809409030405

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

Aim of our study was to investigate the effect of clonidine and galanin (alone or in combination) on growth hormone (GH) secretion in normal subjects and in adult patients with increased somatostatin tone due to chronic daily immunosuppressive glucocorticoid treatment. We studied 7 adult patients undergoing long-term (no less than 6 months) immunosuppressive glucocorticoid treatment for non endocrine diseases (4F, 3M; age 49.7±6.3 years). Six normal adult nonobese subjects (3F, 3M; age 34±2.7 years) served as controls. All subjects underwent the following three tests in random order: 1) iv infusion of clonidine, 150μg in 10 mL of saline, from time 0 to 10 min; 2) iv infusion of synthetic porcine galanin, 500μg in 100 mL of saline from−15 to 30 min; 3) iv infusion of clonidine from 0 to 10 min combined with synthetic porcine galanin iv infusion from−15 to 30 min. Blood samples for GH assay were taken at−15, 0, 15, 30, 45, 60, 90, 120 min. No significant differences in GH absolute values were observed at any time between the three different tests within each group of subjects. Normal subjects showed significantly (p<0.05) higher GH peaks and GH absolute values from 15 to 90 min after galanin alone, clonidine alone and clonidine+galanin with respect to the glucocorticoid-treated patients. The absence of any either synergistic or at least additive effect on GH secretion of galanin and clonidine in conditions of both normal and increased somatostatin tone suggests that also in man, as well as in the rat, the action of galanin on the GH axis may be mediated through alpha-adrenergic pathways.

ISSN:0743-5800    

DOI:10.3109/07435809409030406

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

A 38 years old woman and her first cousin, a 41 year old man, presented both with hypertension, hypokalemia, hyperaldosteronism, and low plasma renin activity in our Hospital. In both patients, plasma and urine aldosterone were constantly above the normal range, even on a high NaCl diet (250 mEq/day), while the plasma aldosterone response to postural changes was normal. In the female patient abdominal ultrasonic scan, CT scan, MRI, and adrenal gland phlebography were normal, but blood from the left adrenal vein contained 1002 pg/ml of aldosterone, versus 91 pg/ml in the contralateral one. Interestingly, the secretion of cortisol was also lateralized (plasma cortisol levels being of 28.8 mcg% in the left, 2.3 mcg% in the right adrenal gland), although neither clinical nor laboratory signs of hypercortisolism were present. Spironolactone treatment (100 mg/daily) completely reversed the syndrome of mineralocorticoid excess. After 2 years, patient has normal blood pressure and serum K+levels.In the male patient MRI showed the picture of marked hyperplasia of the left adrenal gland. Also in this case, blood from the left adrenal vein contained more aldosterone and cortisol (1304 pg/ml and 119.1 mcg% respectively), than the right one (564 pg/ml of aldosterone, and 14.6 mcg% of cortisol, respectively), without clinical or laboratory signs of hypercortisolism. When the left gland was excised, microscopic and macroscopic subcapsular nodules were revealed. After 6 months from surgery, patient is still normotensives, with normal serum K+levels and urinary Na+/K+ratio.

ISSN:0743-5800    

DOI:10.3109/07435809409030407

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

Combination of TSH and Interleukin-6 (IL-6) can stimulate DNA synthesis in FRTL-5 cells. Phorbol 12-miristate 13-acetate (PMA) stimulates G0to G1transition but inhibits G1 to S transition in these cells. We studied the effect of IL-6 on DNA synthesis in TSH-pretreated FRTL-5 cells in the presence or absence of PMA. IL-6 induced DNA synthesis when it was added to TSH-pretreated cells. Further, PMA inhibited the DNA synthesis induced by IL-6. These data might suggest that in FRTL-5 cells IL-6 can show its mitogenic effect in G1phase of their cell cycle progression.

ISSN:0743-5800    

DOI:10.3109/07435809409030408

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

It has been previously shown that acute ethanol (EtOH) exposure in vivo resulted in suppression of serum LH and pituitaryß-LH subunit mRNA levels in castrated male rats. While serum FSH levels also were noted to fall after in vivo, the mRNA forß-FSH was not altered. The aim of the present studies was to determine whether these effects could be accounted for by a direct EtOH effect at pituitary level. To this end we examined the direct effect of EtOH on LH and FSH synthesis and secretion utilizing dispersed anterior pituitary cells from gonadectomized adult male rats. After a 72 hour post dissociation healing period, the cells were exposed to media containing 0 or 200 mg% EtOH for one hour. The media was removed and the cells incubated with EtOH-free media for an additional 1, 3 or 6 hrs. In the EtOH exposed cells, secretion of both LH and FSH increased to>300% (p<0.001) of control. At 6 hrs after withdrawal of EtOH a significant reduction in both LH and FSH secretion was seen. Intracellular content of LH and FSH was unchanged before and after withdrawal of EtOH. Steady state levels ofßLH andßFSH mRNA were unchanged at all time points.In a separate series of experiments, pituitary cells from gonadectomized adult male rats were continuously exposed to different concentrations of EtOH ranging from 0–400 mg% for 3 hrs. LH secretion was stimulated by 400 mgm% EtOH only, while the intracellular content of LH was significantly reduced with the 400 mg% dose. The secretion of FSH was stimulated by 200 mg% and 400 mg% high dose EtOH after 3 hours, with concomitant reduction in FSH pituitary content at both these EtOh dose levels. The mRNA for bothß-LH andßFSH was not different with any dose of EtOH compared to levels of control, non-EtOH exposed cells.We conclude that though there were similarities between in vivo and in vitro LH and FSH responses to EtOH, the differences reported here indicate that the in vivo responses are not totally explained by a direct EtOH effect at pituitary level. Rather, they must, in addition, reflect action at suprapituitary site(s), pituitary effects of EtOH metabolites or condensation products, and/or alterations in LH and FSH clearance.

ISSN:0743-5800    

DOI:10.3109/07435809409030409

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor