年代:1993 |
|
|
Volume 14 issue 1
|
|
211. |
Colloidal carriers for intravenous drug targeting: Plasma protein adsorption patterns on surface‐modified latex particles evaluated by two‐dimensional polyacrylamide gel electrophoresis |
|
ELECTROPHORESIS,
Volume 14,
Issue 1,
1993,
Page 1382-1387
Torsten Blunk,
Denis F. Hochstrasser,
Jean‐Charles Sanchez,
Bernd W. Müller,
Rainer H. Müller,
Preview
|
PDF (634KB)
|
|
摘要:
AbstractTargeting to specific sites of the bodyviacolloidal carriers is sought in order to reduce drug side effects. The adsorption of plasma proteins on intravenously injected particles is regarded as the key factor in explaining their organ distribution: total bound protein, or, more likely, the presence of specific proteins and their conformation, are expected to influence macrophage uptake. Polystyrene beads, 60 nm in diameter, were used as model carriers; their surface was differentially modified by adsorption of increasingly hydrophilic block copolymers, poloxamers 184, 188 and 407. After incubation in plasma, the patterns of protein adsorption onto coated beads were analyzed by high‐resolution two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE). The behavior of some representative proteins was monitored, including albumin, fibrinogen, IgG, factor B and the apolipoproteins, A‐I, A‐IV, C‐III, E and J. The more hydrophobic the particles, the larger the total amount of bound protein. However, this correlation was not valid for all of the analyzed protein species, which proves that it is insufficient to look only at physico‐chemical data to predict organ distribution. On the contrary, it is essential to use 2‐D PAGE to establish the correlation between adsorbed proteins and carrier
ISSN:0173-0835
DOI:10.1002/elps.11501401214
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1993
数据来源: WILEY
|
212. |
Miscellaneous |
|
ELECTROPHORESIS,
Volume 14,
Issue 1,
1993,
Page 1388-1388
Preview
|
PDF (88KB)
|
|
ISSN:0173-0835
DOI:10.1002/elps.11501401215
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1993
数据来源: WILEY
|
213. |
Masthead |
|
ELECTROPHORESIS,
Volume 14,
Issue 1,
1993,
Page -
Preview
|
PDF (62KB)
|
|
ISSN:0173-0835
DOI:10.1002/elps.1150140101
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1993
数据来源: WILEY
|
|