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| 51. |
Construction of a directory of tobacco plasma membrane proteins by combined two‐dimensional gel electrophoresis and protein sequencing |
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ELECTROPHORESIS,
Volume 18,
Issue 3‐4,
1997,
Page 654-660
David Rouquié,
Jean‐Benoǐt Peltier,
Monique Marquis‐Mansion,
Colette Tournaire,
Patrick Doumas,
Michel Rossignol,
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摘要:
AbstractThe polypeptide pattern of the plasma membrane from tobacco was studied by two‐dimensional gel electrophoresis. When using classical carrier ampholyte isoelectric focusing/sodium dodecyl sulfate – polyacrylamide gel electrophoresis (IEF/SDS—PAGE) approximately 400 polypeptide spots were detected after silver staining and computer analysis using the QUEST software. This resolution was sufficient to assess physiological effects such as changes in a phytohormone concentration. By using pH 4–8 immobilized pH gradient (IPG)‐IEF and 10%T SDS‐PAGE gels, approximately 600 polypeptides, corresponding toca.80% of the total population expected, were resolved. This cross‐section of the plasma membrane polypeptide population was mainly constituted by low or intermediate molecular mass (25 to 45 kDa) and acidic (5.2
ISSN:0173-0835
DOI:10.1002/elps.1150180352
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1997
数据来源: WILEY
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| 52. |
Miscellaneous |
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ELECTROPHORESIS,
Volume 18,
Issue 3‐4,
1997,
Page 661-661
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PDF (104KB)
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ISSN:0173-0835
DOI:10.1002/elps.1150180353
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1997
数据来源: WILEY
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| 53. |
Masthead |
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ELECTROPHORESIS,
Volume 18,
Issue 3‐4,
1997,
Page -
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PDF (46KB)
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ISSN:0173-0835
DOI:10.1002/elps.1150180301
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1997
数据来源: WILEY
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Volume 18 issue 3‐4