摘要:

AbstractA computer‐aided analysis based on soft‐laser densitometry of electrophoretic patterns is described. The system has the potential to automatically identify wheat cultivars from normalized sodium dodecyl sulfate – polyacrylamide gel electropherograms. The principle emcompasses autocalibration of the relative mobilities of components of the unknown pattern using standards ofMrthat bracket each pattern on the pictures, automatic identification of peaks and baseline correction, and simplified estimation of the peak intensities. The algorithm provides a normalized array that can be automatically compared with a library of standard cultivar patterns in order to determine those having the highest similarity percentage. The outputs of the program are (1) relative similarity plots which show the percentage of similarity between the unknown cultivar to that of each of the common standard cultivars put in a pattern library, (2) the names of cultivars in the library having the most similar patterns and (3) computer‐generated electrophoretic graphics of these cultivars. This system is not intended to identify closely related cultivars but it is particularly recommended for cultivar identification without well‐trained personnel, familiar with band location and nom

ISSN:0173-0835    

DOI:10.1002/elps.1150090502

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractBy inserting nitrocellulose strips into agarose gels alongside the electrophoresed lanes and passing an electric current perpendicularly in the direction of the strips, highly efficient transfer of DNA bands onto the membrane in the form of concentrated dots is achieved. DNA detection limits by this technique are enhanced, at least three times as visualized by ethidium bromide fluorescence and at least twice more by radiolabeling.

ISSN:0173-0835    

DOI:10.1002/elps.1150090503

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractA motor driven gradient maker based on the commercial model (Jule Inc., Trumbull, CT) was designed for immobilized pH gradient gels to provide small volumes, rapid stirring and delivery, strict volume and temperature control and air exclusion. The device was constructed and by a convenient procedure yields highly reproducible gradients either in solution or on polyacrylamide gels.

ISSN:0173-0835    

DOI:10.1002/elps.1150090504

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractAn improved method for thin‐layer agarose isoelectric focusing of alkaline phosphatases (AP) from human neutrophils is described. The solubilization of AP isoenzymes was studied with four detergents. The best results were obtained after sonication with Zwittergent 3–12 (1 % final concentration), followed by butanol extraction and ultracentrifugation 105 000 ×gfor 1 h. The cytosol can be stored at O °C or −80 °C, but not at −20 °C. Dialysis of the cytosol against a Tris buffer, pH 7.5, was imperative prior to focusing for removal of the detergent. Enzyme visualization is enhanced by incorporation of Zn Cl2(3 mM) into the agarose gel. The focusing patterns consist of two sets of isoenzymes: two main zones with pI6.4 and 6.8 and minor components with pI4.2,

ISSN:0173-0835    

DOI:10.1002/elps.1150090505

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractThe genetic polymorphisms of orosomucoid, ORM1 and ORM2, were analyzed by isoelectric focusing on polyacrylamide gels and subsequent immunoprinting. Sera from 600 unrelated individuals in Taiwan and Japan were examined. Five new alleles, designatedORM1*7,ORM1*8,ORM1*4.2,ORM2*8andORM2*10, were observed together with common and rare alleles that have been found in the Japanese and the Filipinos. As compared with the Japanese, the Taiwanese have a higher frequency ofORM1*1, and a significantly lower frequency ofORM1*2·1

ISSN:0173-0835    

DOI:10.1002/elps.1150090506

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractA procedure is described for the detection of keratinocyte specific proteins. Fibroblasts and keratinocytes were isolated from human skin and radiolabelledin vitro.Samples were separated by two‐dimensional polyacrylamide gel electrophoresis to compare the proteins synthesised by the different types of cultured skin cells. Dual label autoradiography of samples radiolabelled with [35S]methionine and [75Se]selenomethionine was used to identify keratinocyte specific proteins. We report 45 keratinocyte‐specific components and identify some of these proteins. The differential expression of these proteins and their relevance to epidermal differentiation are discus

ISSN:0173-0835    

DOI:10.1002/elps.1150090507

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractStudies from several laboratories involving amino acid analysis and sequencing of theMr35 000 pulmonary surfactant‐associated proteins (SP‐A) have detected hydroxyproline residues. These residues are present in a region with a collagen‐like sequence that has been revealed by direct amino acid sequencing and from the deduced amino acid sequence of the cDNA clones coding for SP‐A. We treated human lung tissue with tunicamycin to blockN‐glycosylation and with 2,2‐dipyridyl to inhibit the hydroxylation of proline residues. The SP‐A synthesized under these conditions showed a shift in apparent molecular weight to 27 000 and 29 000 compared to 29 000 and 31 000 for SP‐A synthesized in the presence of tunicamycin alone. Dipyridyl treatment alone caused an alteration in electrophoretic mobility similar to that seen with tunicamycin, although this was more difficult to evaluate since changes in molecular weight due to glycosylation occurred under these conditions. These results indicate that proline hydroxylation in the collagen‐like portion of SP‐A decreases its elect

ISSN:0173-0835    

DOI:10.1002/elps.1150090508

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractRapid transfer of electrophoretically separated, high molecular weight proteins from fabric‐reinforced, soft, low‐concentration polyacrylamide gels (3.5 %T, 2.6 %C) is described. Polyester fabrics proved useful for gel reinforcement during vertical slab gel electrophoresis. The fabric‐reinforced gel retains its dimension during transfer, equilibration and drying, facilitating comparison of protein bands in the separation gel and transfer m

ISSN:0173-0835    

DOI:10.1002/elps.1150090509

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractAn optimized Coomassie staining procedure, utilizing Coomassie Brilliant Blue G‐250 in phosphoric acid/ammonium sulfate, was applied to ultrathin‐layer isoelectric focusing in 0.18 mm polyacrylamide gels, and sodium dodecyl sulfatepolyacrylamide gel electrophoresis in 0.38 mm polyacrylamide gels, both backed to Gel‐Fix polyester supporting films. After isoelectric focusing staining of gelatin and acidic proteins was better with the phosphoric acid/ammonium sulfate procedure than with conventional organic solvent methods. When applied to gels after sodium dodecyl sulfate‐polyacrylamide gel electrophoresis the sensitivity of the phosphoric acid/ammonium sulfate method was equal to that on conventional staining but lower than on silver s

ISSN:0173-0835    

DOI:10.1002/elps.1150090510

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractSubtyping of the group specific component in secretions of semen and vaginal fluids is impossible with conventional detection systems. By means of a highly sensitive alkaline‐phosphatase secondary antibody system the group specific component can reliably be‐detected in semen stains. Results with vaginal swabs were inconsistent and in saliva stains Gc activity could not be detec

ISSN:0173-0835    

DOI:10.1002/elps.1150090511

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY