摘要:

AbstractNumerical integrations of the partial differential equations describing the time and spatial evolution of isotachophoresis were performed on three different systems, in one case involving reaction kinetics to investigate weak electrolytes.

ISSN:0173-0835    

DOI:10.1002/elps.1150060502

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1985

数据来源: WILEY

摘要:

AbstractThe resolution of angiotensinogen (Mr56 800) linked to a decapeptide, angiotensin I (Mr1295), from the same protein without the decapeptide presents a challenging separation problem. Polyacrylamide gel electrophoresis at an optimized pH of 6.88 is unable to distinguish between the two proteins with statistical significance on the basis of size, but is able to discriminate between them with significance on the basis of their net charge difference. Correspondingly, the proteins are separated in gel electrofocusing with a pIdifference of about 0.1 pH unit. For practical purposes, neither the charge separation by gel electrophoresis nor that by steady‐state electrofocusing seems sufficiently convenient. By contrast, transient state electrofocusing provides a ready means for their separatio

ISSN:0173-0835    

DOI:10.1002/elps.1150060503

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1985

数据来源: WILEY

摘要:

AbstractAn overlay grid was constructed to facilitate the measurement of densiometric scans of proteins in pH gradients formed by electrofocusing. The overlay procedure provides a rapid way for data collection. The relationship between pH and gel distance closely fit a straight line, (R>0.99). The reliability of the system was shown by the close agreement of the linear regressions from six different sets of data. Also, the feasibility of using a similar grid for a nonlinear system was established.

ISSN:0173-0835    

DOI:10.1002/elps.1150060504

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1985

数据来源: WILEY

ISSN:0173-0835    

DOI:10.1002/elps.1150060505

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1985

数据来源: WILEY

摘要:

AbstractSodium dodecyl sulfate‐polyacrylamide gel electrophoresis, and the immunoblotting technique, were applied to structural analysis of a single protein (alpha‐2‐macroglobulin, α‐2‐M) in human serum or plasma without prior purification of this protein. The use of immunoglobulin fractions of four monospecific polyclonal antibody preparations to α‐2‐M in the immunoblotting step showed marked unspecific binding to the proteins fixed onto nitrocellulose. Immunospecific purification of the antibodies by two different methods, and the introduction of biotinylated antibodies and avidin‐peroxidase, removed the background binding. When reduced proteins were fixed onto nitrocellulose a quantitative loss of α‐2‐M antigen reactivity was observed, compared to identical nonreduced samples. The degradation pattern of α‐2‐M in serum or plasma corresponded to the pattern observed with purified α‐2‐M. Thus, the described immunoblotting technique, applied directly to serum or plasma, could detect all not heat‐denaturated α‐2‐M products as seen on Coomassie‐stained polyacry

ISSN:0173-0835    

DOI:10.1002/elps.1150060506

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1985

数据来源: WILEY

摘要:

AbstractIn the classical electrotransfer conditions from sodium dodecyl sulfate‐polyacrylamide gels, calmodulin fails to bind to nitrocellulose membranes. However, under conditions favorizing hydrophobic interactions such as a higher salt concentration, calmodulin can be immobilized on nitrocellulose sheets, thus allowing detection in a complex protein mixtur

ISSN:0173-0835    

DOI:10.1002/elps.1150060507

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1985

数据来源: WILEY

摘要:

AbstractGlucocorticoids are known to enhance fetal lung maturation. Previous studies with whole lung have shown that specific proteins are enhanced by these hormones. At least two effects of glucocorticoids on fetal lung epithelia require the presence of mesenchymal elements. Since the lung consists of several different cell types, further understanding of glucocorticoid effects on fetal lung protein synthesis requires that such studies be carried out with specific cell types. Using two‐dimensional gel electrophoresis, we have examined the synthesis and secretion of proteins by fetal rat lung fibroblasts in the presence and absence of glucocorticoid. Two proteins, fsa and fsb, are enhanced by dexamethasone, appear to be organ specific, and are enhanced only during prenatal and early postnatal life. They are not enhanced by a variety of other hormone

ISSN:0173-0835    

DOI:10.1002/elps.1150060508

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1985

数据来源: WILEY

摘要:

AbstractAnalysis of two‐dimensional electrophoresis patterns demands computer assistance. Specialized machines to read and store the pattern of an autoradiograph or of a “wetgel” into a digital memory are rare. A fast and flexible opto‐mechanical picture reading and storage machine, specially designed for the computer‐aided analysis of two‐dimensional electrophoresis patterns, has been constructed. It is now possible to digitize, for example, a picture of 250 × 400 mm into pixels ofca. 250 × 250 μm with a grey level resolution of 8‐bit within 70 s. Digitizing at significant pixel sizes down to 64 μm is possible at correspondingly longer acquisition times for the areas to be digitized. The design is a flat‐bed scanner. One axis is scanned optomechanically (Galvanometer scanned laser beam), the other axis is scanned mechanically. The area covered by the scanning laser beam has a size of 250 × 400 mm or smaller. The control of the scanner, including pixel density and sampling synchronization is executed by a m

ISSN:0173-0835    

DOI:10.1002/elps.1150060509

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1985

数据来源: WILEY

ISSN:0173-0835    

DOI:10.1002/elps.1150060510

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1985

数据来源: WILEY

ISSN:0173-0835    

DOI:10.1002/elps.1150060501

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1985

数据来源: WILEY