ISSN:0173-0835    

DOI:10.1002/elps.1150050402

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractIn crossed affinoimmunoelectrophoresis with free concanavalin A (Con A) in the first dimension, four orosomucoid components are normally found in human serum. In this study an optimal range of Con A concentration in the first‐dimension gel was defined. The most retarded, fourth component of orosomucoid was shown to be a result of entrapment of Con A‐binding molecules in the affinity precipitate. The affinities of the second, weakly retarded, component and of the third, strongly retarded, component to a Con A binding site were found to be identical. The mobilities in the first‐dimension electrophoresis of the orosomucoid/Con A complexes were found to be significantly different from each other. Compared to the mobility of the nonretarded, first, component of orosomucoid, the second component had a mobility of 40% and the third component had a mobility of 10%. Differences in the antennary carbohydrate structures on the glycosylation sites of orosomucoid are suggested as an explanation for these observa

ISSN:0173-0835    

DOI:10.1002/elps.1150050403

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractUsing panicum mosaic virus (PMV) and several St. Augustine decline strains of PMV (SAD) as test viruses, previously developed procedures of agarose gel electrophoresis (reviewed in P. Serwer,Electrophoresis1983, 3, 375–382) have been used for the first time to detect and characterize plant viruses. PMV and some SAD strains produce two particles that copurify during rate zonal sedimentation, but form separate bands during agarose gel electrophoresis (forms 1 and 2). By measuring electrophoretic mobility (μ) as a function of agarose percentage and correcting for electroosmosis, it was found that: (a) form 1 has a solid support‐free μ (μ0) different from the μ0of form 2, and (b) forms 1 and 2 have the same radius (14.6 ± 0.6nm). Form 1 is indistinguishable from form 2 by either electron microscopy or sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. The μ0of form 1 and the relative amount of forms 1 and 2 vary with the virus strain and can be used to help identify strains. A previously described satellite of PMV and some SAD strains also has two forms. The radius of the satellite of one SAD strain was found to be 7.6 ± 0.3 nm. A procedure of differential gel staining was developed for distinguishing the 14.6 nm virus from its 7.6 nm satellite. After agarose gel electrophoresis of either totally unfractionated or partially fractionated, infected grass extracts, virus particles wer detected without interference from host components. The procedures used here should be useful for rapid disease diagnosis and strain typing of the above and possibly other p

ISSN:0173-0835    

DOI:10.1002/elps.1150050404

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractImmobilized pH gradients covering, in presence of 8 Murea, the intervals pH 4–7, pH 4–8 and pH 4–9 were applied tothe analysis of human serum. Both formulations with constant buffering power and with constant ionic strengthd were tried. Results are presented of the isoelectric focusing step and of a further fractionation of the sample by sodium dodecyl sulfate‐ polyacrylamide gel electrophoresis in a two‐dimensional by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis in a two‐dimen

ISSN:0173-0835    

DOI:10.1002/elps.1150050405

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractAlbumin and glycosylated albumin from serum and urine of 10 diabetic patients have been studied by ultrathin‐layer isoelectric focusing andsilver staining. Both total serum albumin and its glycosylated form were microheterogeneous with a main band focused in the pH 4–5 range; in the case of glycosylated ablumin several cathodic bands with pI's up to 7 were found to form a discretely abundant population among the total species. Some similarites and some striking differences were observed for urinary albumin, which, beside the main unmodified albumin with pI ca. 4.8, was enriched in microheteregeneous more anodal bands (PI4.0–4.7 species). All these anodal bands were tightly bound by concanavalin A‐Sepharose, suggesting their identity as glycosylatedalbumin. these observations indicate that the glycosylation of albumin is not an equimolar reaction, but it can involve many sites of attachement of carbohydrates. The also suggest that albumin species with higher net negative charge and higher content of carbohydrates are preferentially able to pass through the glomerular barrier, providing the descriptive basis of diabetic albuminuria. Their relevance to diabetic nephropathy is a goal for furth

ISSN:0173-0835    

DOI:10.1002/elps.1150050406

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractThe genetic varients of the human protease inhibitor system were examined by isoelecric focusing with a modified immobilized pH gradient. Classifications of PI varietns and PI M subtypes were made in the so‐called PI m8 zone. The common and rare inherited PI M subtypes could be readily distinguishe

ISSN:0173-0835    

DOI:10.1002/elps.1150050407

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractA method is introduced for the determination of thiodiacetic acid in urine by analytical capillary isotachophoresis with colunm coupling. Thiodiacetic acid is one of the final metabolites of carcinogenic vinyl chloride and appears at increased levels in the urine of people exposed to vinyl chloride vapours. The method enables the direct analysis of samples of 10 μl of urine without any pretreatment in about 45 min. The lowest detectable concentration of thiodiacetic acid was about 6 × 10−6mol/liter and the reproducibility of the analyses in the range of 2 ‐ 15 × 10−5mol. per liter was about

ISSN:0173-0835    

DOI:10.1002/elps.1150050408

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractThe method of double‐label autoradiography described by McConkey (Anal. Biochem.1979,96, 39‐‐44) has been updated for the use of the commercial flour. EN3HANCE, and the Lodak films XAR and SB. Fore gels 0.8 mm thick, the input ratio of3h dpm/35S dpm should be 11.1 and the second‐stage authoradiogram should be exposed 5.9 × as long as the flurogram. For gels 0.4 mm thick, the input ratio of3dpm/35dpm should be 5.3 and the second‐stage autoradiogram should be exposed 3.1 × as long as the

ISSN:0173-0835    

DOI:10.1002/elps.1150050409

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractThe use of75Se in combination with35S for double‐label autoradiography has been quantitated. For gels 0.8 mm thick impregnated with EN3HANCE, the input ratio of75Se dpm/35S dpm should be 3.3; and the second stage autoradiogram, if made with an interposed sheet of blackened XAR films as a screen to block35S, should be exposed 7.8x as long as the flourogram for SB film or 5.6 × as long as the fluorogram for XAR film. However, autoradiograms made under these conditions have larger, more diffuse spots than those made with the3H/35S system and the cost of [75Se]selenomethionine is much higher than the cost of [3H]methioni

ISSN:0173-0835    

DOI:10.1002/elps.1150050410

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractProteins from cerebrospinal fluid (CSF) were separated by two‐dimensional electrophoresis (2‐DE), visualized with silver staining and quantitated with computer‐assisted densitometry. This report describes the polypeptide variations observed and their correlations to disease states. CSF from 22 multiple sclerosis (MS) patients was compared to CSF from: 20 non‐inflammatory neurological disease cases (NINDC), 17 inflammatory neurological disease cases (INDC) including a sub‐set of 6 patients with sub‐acute sclerosing panencephalitis (SSPE) and 14 normal volunteers. In a specific comparison between the MS group and the normal volunteers, 32% of the polypeptides varied quantitatively (p<0.05 or greater). The NINDC group resembled the normal volunteers, while the INDC and SSPE groups resembled the MS group. In general, the quantitative changes in the polypeptides analysed in this study permit one to distinguish one disease state from another. In addition to these quantitative alterations, we also report the appearance of additional immunoglobulin light chain species in MS, SSPE,Herpes simplexencephalitis, neurosyphilis, one patient with Shy‐Drager syndrome and one patient with idiopathic orthostatic hypotension. No other disease‐specific qualitative changes were observed in the form of either deletions of proteins normally present, or additional proteins. This survey utilizing 2‐DE has demonstrated protein alterations in the CSF of patients with varied neurological diseases compared with normal CSF, which may prove useful in c

ISSN:0173-0835    

DOI:10.1002/elps.1150050411

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY