摘要:

SUMMARYIn DA strain rats, there are two other MHC class I loci (PaandRT1.Fa) in the vicinity of the classical class I locusRT1.Aa. The Pa antigen is the pregnancy‐associated antigen, and it was detected by antibodies elicited in WF females pregnant by DA males without any other immunization. The Faantigen was detected by a monoclonal antibody raised by alloimmunization. In the present work, the Aa, Faand the Pa antigens have been compared by HPLC peptide mapping and by isoelectric focusing after their isolation by appropriate monoclonal antibodies. All the three antigens are identical in primary structure with respect to lysine, methionine, asparagine and the aromatic amino acid residues, but they differ from one another with respect to glutamic acid and/or aspartic acid residues. The PI values of the antigens differ slightly. All three antigens have two identicalN‐linked glycans, but the Faantigen has an additionalN‐linked glycan. Based on the available amino acid sequence of the Pa antigen, it can be concluded that both Aaand Pa antigens are devoid of glycosylation in the second domain. This lack of glycosylation of the classical antigen Aais unique for the rat, since classical class I antigens of the mouse show glycosylation in the first and second, and sometimes in the third domain, and those in the human, in the first domain only. The high degree of similarity among the Aa, Fa, and Pa molecules that this study indicates is also unique for the rat, since antigens encoded by different class I genes of the same haplotype are quite disparate in the mouse and

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00864.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYThe rabbit MHC class II DP, DQ, and DR alpha and beta chain genes were transfected into murine B lymphoma cells. The transfected cells expressed R‐DQ and R‐DR molecules on the cell surface but they did not express the R‐DP genes either on the cell surface or at the level of mRNA. Northern blot analyses showed that the R‐DP genes were expressed, albeit at low levels, in rabbit spleen. Similar analyses showed that the R‐DQ and R‐DR genes were expressed at high levels in rabbit spleen. A new monoclonal anti‐rabbit class II antibody, RDR34, has been developed and shown to react with the R‐DR transfected cells and not with the R‐DQ transfected cells. The previously described monoclonal anti‐rabbit class II antibody, 2C4, reacted with the R‐DQ transfected cells and not with the R‐DR transfected cells. Thus, 2C4 and RDR34 MAb's are specific for the R‐DQ and R‐DR molecules, respectively. Each of the antibodies reacted with approximately 50% of rabbit spleen cells as shown by immunof

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00865.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYBy the isolation of overlapping cosmid clones and ‘chromosome walking’ studies from theH‐2Kkgene, we have obtained cosmid clones encoding theH‐2K1kgene from two separate cosmid libraries. The nucleotide sequence of one of the clones was determined. The clonedH‐2K1kgene could be transcribedin vitroto give a normal H‐2 class I mRNA of 1.7 kb. However, the deletion of four nucleotides in exon 3 of theH‐2K1kgene results in a translation termination codon at the beginning of exon 4. In agreement with this, when expressed in human cells, theH‐2K1kgene gave a truncated, cytoplasmic polypeptide ofMr36,000. Therefore, although theH‐2K1kgene is homologous to other class I MHC genes in its molecular organization and nucleotide sequence, it is a pseudogene. When compared to the nucleotide sequence of theH‐2Kkgene, theH‐2K1kgene has undergone many substitutions of methylated CpG residues (meCpG). This represents further evidence to suggest that

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00866.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYCell surface expression of class II major histocompatibility complex encoded (la) molecules depends on association of the component α and β chain into a stable heterodimer. Studies on cell lines transfected with MHC class II genes have revealed important limitations on the assembly of haplotype‐mismatched Aα:Aβ complex. In order to study α: β chain pairing restrictionsin vivoa number of lines of transgenic mice carrying the Aαkgene were generated. The transgene was studied in the context ofH‐2b, H‐2s, H‐24, H‐2u, H‐2fandH‐2vhaplotypes. Initial FACS analysis of spleen and peripheral blood cells showed noAαkexpression. Spleen cells stimulated with LPS and analysed by FACS showed Aαkexpression in three different H‐2bstrains as well as H‐2u, but not in others. These data indicate that Aαkcan associate withAβband Aαk, b

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00867.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY