摘要:

SUMMARYEpa‐1‐specific cytotoxic T lymphocytes (CTL) lyse epidermal cells (EC) of different Epa‐1+H‐2kstrains, such as AKR, CBA, C58, and RF, at different levels. We used an H‐2Kk‐specific monoclonal antibody (mAb) to test the hypothesis that this phenomenon is due to differences in the H‐2‐restricting element. Initially, we established the specificity of this mAb for the Epa‐1‐restricting element by demonstrating its capacity to inhibit the lysis of CBA EC by Epa‐1‐specific CTL. We then used it as the probe in a cellular radioimmunoassay to quantify the expression of the restricting element by EC of differentH‐2kstrains. We found that C58 and RF EC bound significantly less of the mAb than did CBA EC. Although AKR also bound less of the mAb than did CBA EC, the difference was not statistically significant. To examine the generality of this phenomenon, we quantified the expression of Kkantigens on spleen cells (SC) of the same four strains. We found that RF SC, but not AKR or C58 SC, bound significantly less of the KkmAb than did CBA SC. Thus, the differential CTL lysis of Epa‐1+EC of different strains probably reflects differences in expression of the H‐2‐restricting element rat

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00375.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SUMMARYThe genetic control of the immune response to bovine insulin (BI) in the rat was investigated. As a result of experiments utilizing two intra‐MHC recombinant rats and of blocking experiments with monoclonal antibodies against MHC class II antigens, an immune response gene for BI in the rat could be mapped to theRT1.Bβloc

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00376.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00377.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Book reviewed in this article:J. W. Goding:Monoclonal antibodies: Principles and Practice. Production and Application of Monoclonal Antibodies in Cell Biology, Biochemistry and Immunology.W. I. Morrison(Ed.):The Ruminant Immune System in Health and Disease.

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00378.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY