摘要:

SUMMARYClass I and II histocompatibility antigen expression was studied in cryostat sections of biopsy tissues from 15 patients diagnosed as suffering from malignant melanoma, using monoclonal antibodies against HLA class I and II mono‐morphic determinants and an indirect immunofluorescence technique. Class I antigens were detected in three of the four primary melanomas and in five of the eleven metastatic melanomas. Class II antigens were expressed only in metastatic melanomas, in three out of eleven cases.Some tumour cell suspensions were obtained and short‐term cultures were established. Radiobinding and immunoprecipitation studies were carried out in two cases, named M6 and M8. The results were comparable to those obtained with direct immunofluorescence. We modulated the expression of class I and II HLA antigens with interferon in M6 when adapted to tissue culture. This melanoma was class I and II negative; after IFNγ treatment it became strongly positive for class I and II antigens. In addition we have demonstrated, using Southern blot analysis with the restriction enzymes PvuII and EcoRI, that the M6 melanoma does not have any detectable alterations in its class IIβ

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01104.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYImmunohistochemical analysis for non‐polymorphic determinants of HLA class I or class II antigens has been greatly facilitated by the use of monoclonal antibodies. Studies on the distribution of these antigens in tumour lesions emphasize their role in the tumour‐host interaction and in tumour histo‐pathology, especially as additional markers in the assessment of prognosis of melanoma patients. Changes in the expression of HLA antigens on tumour cellsin vitroas induced by γinterferon may also occurin vivoas a result of local production by the T lymphocytic infiltrate in t

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01105.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYThe expression of class I and class II HLA antigens has been studied on primary and metastatic human melanomas, and on cell clones derived from the latter. A panel of monoclonal antibodies and flow cytofluorometric analysis were used to evaluate the presence of HLA‐A, B, C and ‐DR, DQ antigens on freshly isolated tumour cells. HLA class I antigens were present on 91% and 93% of primary and metastatic tumours, respectively. Sixty per cent of primary and 50% of metastatic melanomas expressed HLA‐DR antigens, whereas 38% and 21% of cases were positive for HLA‐DQ. A marked heterogeneity was evident among primary and metastatic lesions for expression of class I and II antigens. Similiar findings were obtained by analysing the phenotype of melanoma clones which indicates that a marked antigenic heterogeneity for class I and II HLA antigens occurs even among clones isolated from short‐term cultures of metastatic

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01106.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYDNAs from human malignant melanoma cells and autologous peripheral blood lymphocytes were evaluated by Southern blot analysis with probes for class I and II HLA genes. DNA of melanoma cells digested with PvuII, EcoRI and BglI and hybridized with a DRB probe showed a loss of several fragments when compared with DNA from lymphocytes. The same DNAs were not distinguishable when hybridized with a DQB probe. Analysis of melanoma and autologous lymphocyte DNAs from the same patient with a class I cDNA, after digestion with several endonucleases, revealed a further loss of fragments in melanoma cells. Comparison of restriction fragment patterns of melanoma and lymphocytes with those of homozygous, serologically‐typed cell lines indicated that melanoma cells have lost fragments diagnostic of DR2 and A1 antigens. A densitometric analysis of signals of several oncogenes in comparison with that of DR indicated that a duplication of the remaining DR allele had occurred in melanoma cell

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01107.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYHLA class I and II antigen expression was studied in 19 cases of primary infiltrating ductal carcinoma of the breast. An indirect immunofluorescence technique was used on cryostat sections with monoclonal antibodies directed against HLA class I and II monomorphic determinants. Of the 19 cases studied, 17 were positive for class I antigen expression and two were negative. Class I HLA antigen expression was found to be clearly heterogeneous: in ten of these tumours more than 75% of the cells were class I positive; in two the percentage was decreased to between 50% and 75%; in five tumours it was less than 50%. With respect to class II HLA antigen expression, eight breast tumours were totally negative while two were strongly positive. (50‐75%) and the nine remaining cases were less than 25% positive.In addition, radioassay techniques were employed to determine the presence of oestrogen and progestagen receptors. The distribution of these receptors was not correlated with HLA class I or II antigen expression, nor could any relationship be demonstrated between the degree of histological differentiation of the tumours and their invasivenes

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01108.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYWe investigated the effect of gamma interferon and phorbol ester (TPA), on the expression of HLA class II molecules of myeloid leukaemic cell lines K562, U937, KG‐1, HG60 and ML‐2. Gamma interferon induced the expression of HLA‐DR but not HLA‐DQ on HL‐60 and ML‐2, increased the expression of HLA‐DR and DQ on U937 and induced the expression of HLA‐DQ on KG‐1. TPA treatment did not affect the expression of HLA class II antigens on U937 and KG‐I and induced the expression of HLA‐DR and HLA‐DQ on HL‐60 and ML‐2. TPA treatment did not affect the HLA phenotype of K562 but gamma interferon did induce HLA class I molecules. Thus, gamma interferon cannot only increase the expression of HLA products already expressed on the cells but can also induce thede novosynthesis of these molecules on m

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01109.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYThe antigenic specificities of H‐2‐restricted, tumour‐specific cytolytic T lymphocytes (CTL) were studied at the molecular level using CTL from BALB.B and C57BL/6 (H‐2b) mice sensitized to anH‐2bGross murine leukaemia virus (MuLV)‐induced tumour. Target cells were produced by the double trans‐fection of mouse L cells (H‐29 with the clonedH‐2KborH‐2Pgene and retro‐viral DNA derived from a molecular clone of Akv MuLV (closely related to Gross MuLV). Doubly transfected L cells which express either H‐2Kbor H‐2Dbantigen and retroviral antigens are lysed in a virus‐specific manner by Gross MuLV‐immune CTL. The existence of two independent Gross MuLV‐immune CTL subpopulations, one restricted by H‐2Kband the other by H‐2Db, is thus confirmed. Gross MuLV‐immune CTL from both BALB.B and C57BL/6 mice killed L cells that express Akv MuLVgaggene products and H‐2Kbor H‐2Dbantigen. In contrast, only CTL from C57BL/6 mice killed L cells that express Akv MuLVenvgene products and H‐2Kbor H‐2Db. This indicates that specific recognition of MuLV‐induced antigens by CTL can be selectiv

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01110.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01111.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYThe kinetics of unrestricted killing of normal and leukaemic lymphocyte target cells by a Qa‐lb‐specific murine cytotoxic T lymphocyte (CTL) clone were evaluated in a manner analogous to enzyme kinetic assays in which the effector and target cells corresponded to the enzyme and substrate, respectively. In order to apply the enzyme‐substrate analogy to clonal cytolytic reactions, it was first established that the lytic reactions exhibited initial steady‐state velocity of lysis at the effector and target cell concentrations used. The lytic reaction maintained linearity for velocity of lysis during the first 90 min of incubation, then plateaued.Vmax(the maximal rate of target cell lysis achieved by a given effector population) andK, (the target cell number resulting in 1/2Vmax) values were determined over a wide range of target and effector cell concentrations. Both parameters were found to be directly proportional to the number of effector cells. At a given concentration of cloned CTL, the lytic parameters ofVmaxandK, were not significantly different for normal or leukaemic target cells that express Qa‐lb. Additional kinetic parameters for lysis of normal and leukaemic target cells by a cloned CTL were also compared. The lytic efficiency of the CTL clone (i.e. maximal killing rate with an infinite number of targets) and the intrinsic anity between effector and target cells were the same with either normal or leukaemic targets. However, the maximal lysis of target cells at an infinite number of effectors was significantly less for normal compared with leukaemic targets. This suggests that the normal target cells were more heterogeneous in their expression of the target (Qa‐1b, antigen. Enzyme‐like kinetic analysis of cell‐mediated lysis reactions can be useful for comparing the relative affinities of effector and target cells obtained from

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01112.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYNaturally occurring H‐2‐specific antibodies can be detected rather frequently in sera of non‐alloimmunized mice by sufficiently sensitive techniques (Cerny‐Provazniket al., 1985a; Cerny‐Provaznik&Ivanyi, 1985). In this report, we summarize our experiences with the preparation of monoclonal anti‐H‐2 antibodies obtained from hybridization experiments from non‐alloimmunized mice. From a total of 30 spleen cell hybridization experiments, we could isolate only four anti‐H‐2 monoclonal antibodies (mAb). Two of the m Ab are described in this report. Monoclonal antibody By‐2 is anti‐Kfand mAb By‐3 is anti‐Db, D5. We investigated which conditions favour the isolation of monoclonal H‐2‐specific antibodies from non‐alloimmunized mice. The presence of naturally occurring serum antibodies, the age of the spleen donor mouse or non‐specific B cell stimulation were not critical for the isolation of natural anti‐H‐2 mAb. We hypothesise that the ‘natural’ H‐2‐specific antibodies represent compartments of the B cell repertoire which were trigge

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01113.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY