摘要:

SUMMARYSimian B and T lymphoid cell lines were shown to maintain surface markers found on mature lymphocytesin vivo. The T lymphoid cell lines expressed Ia‐like antigens on their surfaces, further suggesting that they represent mature, activated T cells. These Ia antigens show a structural similarity to Ia on human cells although some diversity exists. The Ia antigen expressed on T lymphoid cell lines was shown to be very similar to those on B lymphoid cell lines. Owl monkey and marmoset T lymphoid cell lines were also shown to express a VHimmunoglobulin‐related determinant, a marker which is thought to be associated with T cell antigen receptor. Owl monkey and marmoset T cell lines express a surface antigen which identifies the sheep erythrocyte receptor on human T cells and some of these lines express an antigen found on human helper T cells. It is noteworthy that substantial conservation of surface components has occurred within primate evolution such that monoclonal antibodies to human Ia, OKT‐11a and Leu 3a markers can be used to type lymphocytes of lower pri

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1982.tb00976.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SUMMARYImmunogenetics of theBasileaallotype were investigated. It was shown that theBasileagene is controlled at a locus identical with, or closely linked to, theAbkappa allotypic locus.Basilea‐positive molecules have been physically separated by immunoabsorption from molecules carrying the λ chain markers, c7 and c21. Injection of newbornBasileahomozygotes with anti‐Basileaimmune serum results in suppression of the synthesis ofBasilea‐positive molecules, but does not affect the synthesis of the λ chain, or of the heavy chain allotypic markers. Allotype‐suppressedBasileahomozygotes do not recognize either theBasileaallotype or the kappa isotype as ‘self’ and make anti‐Basileaand anti‐kappa antibody upon immunization withBasileaantigen. The specificity of this antibody was identical with that of a heterologous anti‐kappa immune serum raised in a goat and made specific by absorption with purified lambda material. These results prove thatBasileagene products are of kappa isotype and are controlled at t

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1982.tb00977.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SUMMARYThe B6‐C.H‐2bm12(bm12) strain has previously been described as a spontaneously occurring mutant wherein the parental C57BL/6 Iabspecificities (Ia.3, 8, 9, 15 and 20) are absent or reduced in amount. To further characterize the bm12mutant the antigenic phenotype of LPS‐activated bm12blast cells was determined with monoclonal and conventional anti‐Ia antibodies. By direct testing and absorption the public Ia.8, 9 and 15 specificities were readily detected on both parental C57BL/6 and mutant bm12blast cells, and the amounts of these antigens on activated bm12cells were similar to or less than those found on C57BL/6 blast cells. The findings demonstrate that theIa‐1mutation has not resulted in an absolute loss of these specificities, but has altered their expression, or their accessibility to antibody on resting cells. By contrast, the private Ia. 20 specificity and another specificity (designated Iab) could not be detected on bm12bl

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1982.tb00978.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SUMMARYData presented here defines the map ofH‐2associated genes which affect the glucocorticoid‐induced cleft palate frequency to the regions H‐2K to I‐B and G to H‐2D. This was done by observing in four congenic strains the frequency of cleft palate induced by 160 mg/kg of dexamethasone administered to pregnant females on day 12 of gestation. The strains used were B10.A/SgSn, C57BL/10Sn, B10.A(5R)/SgSn and B10.A(18R)/Sg. Additionally the cleft palate frequency was observed in a large number of litters from saline‐treated pregnant females. The cleft palate frequency in this control was very low and the rank of strains was the same as for the glucocorticoid‐induced cleft palate frequency. This observation suggests that the induced cleft palate frequency reflects the spontaneous occurrence of isolated cleft palate in

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1982.tb00979.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SUMMARYAge‐dependent changes, during adult life, in suppressor capacity and B cell responsiveness have been known for some time. In this paper, an age dependent change in helper function, is being reported. A Th‐2 type of helper effect can be observed in tissue culture of spleen cells from animals sensitized with a macromolecule. An indirect plaque‐forming response to the hapten occurs when the spleen cells are exposed to the sensitizing macromoleculeandto the hapten conjugated to a carrier, structurally unrelated to the sensitizing macromolecule. There is considerable polymorphism in the age at which this effect is first demonstrable and at which it reaches mature levels. Mice of inbred strains, with various defects in the suppressor cell circuit (SJL/J, MRL/MpJ‐lpr/lpr, BXSB/MpJ) show an accelerated development of Th‐2 helper capacity in the indirect plaque‐formi

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1982.tb00980.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SUMMARYExperiments were conducted to investigate the non‐H‐2genetic effects on experimental autoimmune thyroiditis (EAT). Strains having C3H or BALB backgroud in general produced higher autoimmune responses to mouse thyroglobulin (MTg) than the B10 or A strains. Comparisons of C3H and B10 congenic strains carrying similarH‐2haplotypes demonstrated that the C3H congenics had significantly higher MTg antibody titres and more severe thyroid damage, even when the strains carry the low responderH‐2haplotypes. These observations show that non‐H‐2gene(s) influences EAT, in addition to genes

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1982.tb00981.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SUMMARYD‐25 is a H‐2 alloantiserum produced in (B10.D2 x C3H.NB) (H‐2dx H‐2p)F1 mice after immunization with B10.RIII(H‐2r) cells, and which is known to recognize the H‐2.25 public antigen onH‐2khaplotypes. The ‘anomalous’ reaction of D‐25 with a partially purified deoxycholate‐solubilized glycoprotein of B10‐1 (H‐2b) fibrosarcoma was studied with various biochemical techniques. The125I‐labelled precipitates were analysed both in one‐ and two‐dimensional SDS‐PAGE and by a partial proteolysis peptide mapping (Cleveland's mapping). The results indicate first that the D‐25‐related antigen was a genuine H‐2 antigen normally associated with the B2‐microglobulin, second that this antigen was borne by the Kbbut not Dbgene products, and finally that D‐25 was able to precipitate the same antigen on normalH‐2bspleen cells. We conclude that this serum recognized a normal H‐2 specificity shared byH‐2bandH‐2rhaplotypes and tentatively identified as the public antigen H‐2.54 which for the first time could be assi

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1982.tb00982.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SUMMARYBALB/cAnN and C3H/HeN mice were primed and challenged with DNP‐Ficoll, a thymic independent (TI‐2) antigen. They developed significant carrier‐specific IgG memory responses: DNP‐pneumococcal polysaccharide or DNP‐hemocyanin challange did not elicit memory responses. NZB/B1NJ mice failed to develop this memory response. Genetically controlled events apparently regulate the phenotypic expression of th

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1982.tb00983.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SUMMARYAmong 290 F2 progeny of an r10 x ACP cross were two recombinants which allowed the loci for glyoxalase‐1 and neuraminidase‐1 to be mapped relative to theRT1.Aanddw‐3loci in the major histocompatibility complex (MHC) of the rat. In 673 progeny of the same cross there was a recombinant betweenftanddw‐3, and in 403 progeny of the backcross BY1 x (BY1 x BDIX)F1 there was another recombinant betweenftanddw‐3. These data, combined with those from previous studies, provide the information for constructing a detailed map of the rat major histocompatibility complex: the gene order and size in the rat are very similar to those in the mouse and different from those in man and in the other species that have been studied. Comparison of the structures of the MHC in the various species leads to a hypothesis about the evolution of the MHC which involves sequential duplications of the genes coding for class I and class II loci and an inversion in the prototypic muridae which placed the class II loci between the cla

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1982.tb00984.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY