摘要:

SUMMARYSerum IgG (7S) levels differed significantly for chickens from 10 different inbred lines. Within lines differences between B blood groups were statistically significant.The genetic control of serum IgG was further examined using birds fromBcomplex haplotypes marked at theBlocus and theIr‐GATlocus. Birds from each of five subgroup haplotypes (B1B1Ir‐GAT‐Loand‐Hi, B19B19Ir‐GAT‐Loand‐Hi, andB2B2Ir‐GATintermediate) were tested for levels of serum IgG at 3, 6, 9, and 21 weeks of age. The rate and level of IgG reached in the serum was more than two‐fold greater in the GAT‐Hi birds than in the GAT‐Lo. The Ir region of the B complex exerts some control over the ontogenesis of IgG, though most of the genetic variation seems not to be

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00949.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYThe phylogenetic distribution of antigens present on human lymphocytes was investigated by incubating human or simian cells with murine anti‐human monoclonal antibodies and then determining the level of reactivity with a radiolabelled anti‐murine IgG reagent. The monoclonal antibodies used were specific for a T‐cell antigen, lymphoid and lymphoid:myeloid antigens, Ia antigens, and β2 microglobulin. The cells examined included B‐ and T‐lymphoblastoid cell lines and fresh peripheral blood lymphocytes separated by sheep erythrocyte rosetting into T‐cell and non T‐cell fractions. Results of these studies showed that the antibodies gave complete cross‐reactivity with gorilla and chimpanzee cells while B‐cell lines of orangutan origin had lost lymphoid and β2 microglobulin markers. Gibbon cells and cells of Old World and New World monkeys reacted strongly only with monoclonal antibodies against Ia antigenic determinants. These Ia antigens were found on the non T‐cell fraction of fresh peripheral lymphocytes, on B‐cell lines and on some virus induced T‐cell tumour lines. Immunoprecipitation analysis using the anti‐Ia antibodies showed a degree of molecular diversity on owl monkey and marmoset cells compared to the Ia antigens

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00950.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYThe effect of incompatibility for Mls determinants was studied in lethal graft‐versus‐host reaction (GVHR) in the mouse. GVHR was induced in adult recipients of the following H‐2kstrains: (AKR x B10.BR)F1 (MlsB/Mlsb); (C3H x B10.BR)F1 (Mlsc/Mlsb); (CBA/J x B10.BR)F1 (Mlsd/Mlsb) and (CBA/H x B10.BR)F1 (Mlsb). Recipient mice were heavily irradiated and grafted with bone marrow and spleen cells from H‐2 compatible B10.BR (H‐2k, Mlsb) or H‐2 incompatible B10.D2 and B10 donors were normal, while those from B10.BR donors were either normal or pre‐immunized against the recipient strains. In all experiments the survival of recipients with Mlsa/Mlsband Mlsd/Mlsbphenotypes, and only in one experiment of those with Mlsc/Mlsbphenotype was greater and/or the survival time longer than that of recipients expressing only Mlsb. However, late deaths (>120 days post grafting) observed after grafting of normal B10.BR cells were more frequent in Mlsd/Mlsbthan in Mlsbstrains. On the other hand, when B10.BR donor cells were pre‐immunized against H‐2kcompatible (AKR x B10.BR)F1 (Mlsa/Mlsb) or (CBA/J x B10.BR)F1 (Mlsd/Mlsb) strains, the survival time of H‐2 incompatible (B10 x B10.BR)F1 (H‐2b/k, Mlsb) recipients was longer than when donor cells were pre‐immunized against (CBA/H x B10.BR)F1 (Mlsb) strain. We conclude that donor incompatibility for Mlsaor Mlsdor donor‐pre‐immunization against Mlsaor Mlsdexerts a protective effect on lethal GVHR developed across non‐H‐2 or H‐2 barriers; the protective effect of Mlscis less efficient or absent. The Mls‐induced protective effect shows the following properties: (a) efficiencyin vivocorrelates with the capacity of the corresponding alleles to stimulate anin vitroMLR; (b) is efficient in either primary or secondary response to other minor antigens; (c) is not H‐2 restricted; (d) is nonspecific; (e) disappears late after grafting; (f) with respect to the genetic background, the early protective effect is followed, late after grafting, by an opposite effect which increases the mortality, suggesting thatM/slocus determinants are capable of activating several cell population

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00951.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYDorso‐ventral vaginal septa were observed in congenic mouse strains. Strain C57BL/10Sn, H‐2b, had the highest frequency, 22%; B10.A/SgSn, H‐2a, had the lowest frequency, 4%,P<0.001. The frequency was about 17% in the F1, females. Septa were found in 16% and 15% of the B10.D2/nSn and B10.BR/SgSn strains, respectively, indicating that at least two loci within the H‐2 complex influence its fr

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00952.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYC3H/HeN x NZB/BIN F1mice were primed and challenged with DNP‐Ficoll, a thymic independent (TI‐2) antigen. They developed strong IgM and IgG carrier‐specific memory responses: DNP‐pneumococcal polysaccharide or DNP‐haemocyanin challenge did not elicit memory responses. The IgG response component appeared to be inherited dominantly from the C3H/HeN parent. The IgM component resulted from genetic en

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00953.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYThe segregation of genes controlling ECIA susceptibility and the level of immune response to native calf type II collagen were determined in the F2 progeny of matings between WF (RTIu/u; ECIA‐susceptible; high responders) and LEW.B3 (RT1n/n; ECIA‐resistant; low to intermediate responders). RT1n/nF2 progeny showed resistance to ECIA, low skin test reactivity to type II collagen and intermediate levels of IgG anti‐collagen antibodies (‐log2of 6.2 ± 2.6; mean ± SD,n= 10). RT1u/uand RT1u/uF2 progeny were susceptible to ECIA and were high responders to type II collagen by skin testing and IgG antibody titres (‐log2of 12.1 ± 1.3, mean ± SD,n= 26). Although all rats that developed arthritis were also high responders to type II collagen one group of immature F2 progeny, RT1u/uand RT1u/n, showed high anti‐collagen immune responses in the absence of detectable arthritis. The data indicate that genes linked to RT1.A control susceptibility to ECIA and at least part of the immune response to native calf type II collagen in WF

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00954.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYMice bearing the I‐A subregion mutation bm12 were immunized and challenged with the α‐subunit of human adult haemoglobin. Under conditions in which parental B6/Kh mice respond, B6.C‐H‐2bm12mice are inhibited nearly 100% in their ability to respond to challenge to the α‐chain of haemoglobin. D2.GD mice which express a variant Ae(Eβ) polypeptide of the I‐E subregion can respond as well as B10.D2 mice to both subunits (α‐ and β‐) of haemoglobin. These observations as well as other genetic mapping data confirm the I‐A mapping of α‐chain‐specificIrgenes and extend the genetic fine mapping to theAβgene within the I‐A subregion or a combinatorial Ia determinant generated by an interaction ofAαandAβ. In addition they implicate the Ia.8 specificity in determining immune respon

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00955.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYA long‐termin vitrogrown T cell line derived from a cotton‐topped marmoset(Saguinus oedipus)) infected withHerpesvirus saimiriwas found to share surface antigens with human amplifier T cells and to augment the capacity of human B cells to secrete immunoglobulin. This is the first demonstration of T/B collaboration across such a large phylogenetic barrier and might have interesting implications for understanding the nature of molecular interactions mediating cell/cell cooperat

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00956.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYElectrophoretic and serologic investigations of C4 in serum samples from a family material, showed that a weak, or partial, inhibition of anti‐Rg sera, was an inherited property of some of the C4 haplotype products. The weak Rg activity was strongly, but not absolutely associated with theC4 FIandC4 Ihaplotype

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00957.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYA Friend virus‐induced tumour of BALB/c (H‐2d) origin, HFL/d, was examined for the expression of alien H‐2 antigens. The alloantigens on HFL/d were typed by generating CTL in primary MLC with HFL/d as stimulator and measuring reactivity to targets with known H‐2 antigens, and confirmed by assessing recognition of HFL/d targets by CTL generated in primary MLC with stimulators expressing known H‐2 antigens. Potential cross‐reactivities between alloantigens were analysed by cold‐target inhibition experiments. BALB/c cells stimulated with HFL/d lysed H‐2btargets, and BALB/c anti‐H‐2bCTL lysed HFL/d; analysis with recombinant haplotypes demonstrated both H‐2Kband H‐2Dbalien antigens on HFL/d. C57BL/6 (H‐2b) cells stimulated with HFL/d recognized H‐2Kd, H‐2Dd, and an additional determinant unique to HFL/d. (BALB/c x B6)F1cells also recognised a unique HFL/d determinant not of H‐2bor H‐2dorigin. These unique determinants, which induced a strong cytotoxic response in primary MLC, were not shared by BALB/c or B6 tumours induced by cross‐reactive FMR viruses. Thus, HFL/d expressed the K and D antigens of its strain of origin, two typed alien H‐2 antigens, and at least one other untyped antigen which may represent an additional H‐2 determinant. These studies further demonstrate the utility of examining the reactivity of CTL generated in primary MLC to probe

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00958.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY