摘要:

SUMMARYThein vitroproliferative response of T cells primed with human thyroglobulin (Tg) was compared in 11 independent haplotypes on B10 background. B10.K and B10.S mice were the most responsive, whereas, with the exception of B10.PL (H‐2u, all other B10 congenics were intermediate responders. The two best responders toin vitrochallenge with human Tg, of thekand s haplotype, are the same as those showingH‐2‐linkedsusceptibility to induction of experimental autoimmune thyroiditis (EAT) with mouse Tg. Since shared epitopes on human and mouse Tgs have been shown to be thyroiditogenic by adoptive transfer studies in CBA (H‐2k) mice, the findings indicate that shared epitopes may be studied in appropriate (i.e. EAT‐susceptible) strains of mice. Therefore, we proceeded to develop methods to produce T‐cell lines and hybridomas to human Tg in B10.K and B10.S mice, test their cross‐reactivity to heterologous Tgs and their Ia restriction patterns. By using antigen‐presenting cells from recombinant strains, we identified restriction elements encoded by theI‐Asubregion alone and a combinatorial molecule from theI

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00887.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYTwenty‐two subjects (11 HLA A1 B8 DR3, 11 non‐A1 B8 DR3) were tested for the capacity of their lymphocytes to express Tac molecules and interleukin‐2 (IL‐2) receptors (quantified using radiolabelled IL‐2) after mitogen stimulation. Ten of these subjects (five Al B8 DR3 and five non‐A1 B8 DR3) were also tested for the ability of their lymphocytes to proliferate under IL‐2 stimulation. Al B8 DR3 subjects express a normal number of high‐affinity IL‐2 receptor sites, but the affinity of these receptors sites is significantly increased. Unexpectedly, Al B8 DR3 lymphoblasts show a lower response to IL‐2 than non‐Al B8 DR3 for high dose

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00888.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYInsulin‐dependent diabetes mellitus (IDDM) is associated with several DR3‐ or DR4‐containing ancestral haplotypes (AHs). Using pulsed field gel electrophoresis (PFGE), long range maps of 35 haplotypes have been derived and classified. Two diabetogenic DR3‐containing AHs (8.1 and 18.2) possess deletions in the central non‐HLA region; these have not been found on non‐diabetogenic AHs tested to date. In addition, 8.1 and 18.2 also carry other deletions not found on other AHs. Three DR4 containing AH lack a Not 1 site, which may imply excision of an unidentified gene. These and other data suggest that deletions may be relevant to the pathogenesis of autoimmune disease, possibly through causing quantitative differences in autoimmune responses involved in IDDM. The MHC contains several regions of potential interest in relation to susceptibility to IDDM; these may explain the association with only certain DR3‐ and DR4‐carrying AH and DR3,4 heterozygosity in terms of cis and trans interactions. On the other hand, the class I1 region may be particularly importan

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00889.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYSoluble class I molecules were immunoprecipitated from human sweat and serum using the BB7.7 monoclonal antibody (mAb) coupled to immunomagnetic beads. Molecules were analysed biochemically on SDS‐PAGE gels and finally by 1D‐isoelectric‐focusing (IEF). Serum‐ and sweat‐HLA IEF‐band patterns of the same individual were fully identical, showing that HLA excreted in sweat possess polymorphic structures like those in serum. Quantitatively, we used a highly sensitive competitive enzyme‐linked immunosorbent (ELISA) assay to determine soluble class I concentrations. The first group was that of non‐HLA‐A9 and ‐Bw62 sera, which were found to contain HLA levels with a mean concentration of 0.82 ± 0.63 μg/ml (n= 44). However, sera that were HLA‐A23 or −24 (splits of HLA‐A9) contained higher levels, with a mean of 3.2 ± 0.94 μg/ml (n= 20). Similarly, HLA‐Bw62 individuals had a higher mean of 2.05 ± 0.65 μg/ml (n= 10). The difference of the HLA‐A9 group to the first group was statistically highly significant,P<0.0001, and that of the HLA‐Bw62 to the first was also significant,P<0.004. Individuals who were both HLA‐A9 and ‐Bw62 (n =5) did not express significantly higher levels than those who only had one of these specificities. Sweat HLA levels had a mean of 0.42 ± 0.4 μg/ml (n= 10). These results show for the first time that soluble class I peptides are excreted in relatively high concentrations in sweat and possess polymorphic structures identical to those of serum HLA and t

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00890.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYIntracytoplasmic μ‐positive pre‐B cells (μ+VH315−cells) transformed with Abelson virus continuously produced cells (μ+VH315+cells) that were double‐stained by anti‐μ and anti‐VH315 antibodies which specifically recognized the immunoglobulin heavy chain variable region identical or closely related to that of MOPC315 myeloma protein. Southern blot analysis indicated that the μ+VH315+cells were generated from the μ+VH315‐ cells by the VH315·D·JH2to a germline JH3joining on the non‐productive VH315·D·JH2allele, which resulted in the production of μ‐chains with variable region detectable by anti‐VH315 antibodies. Therefore, it is strongly indicated that VHDJHto JHjoining is not blocked in μ‐chain‐producing pre‐B cells, and thus is

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00891.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYThree HLA‐DR3‐linked polymorphisms of the DPA, DPB and DRB genes, previously associated with myasthenia gravis or coeliac disease, have been examined in Caucasian patients with Graves' disease. The patients did not differ from healthy DR3 subjects, indicating an absence of any association of Graves' disease with specific DR3 subty

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00892.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYTwo human alloantisera, previously described as possibly detecting new beta 2‐microglobulin associated proteins, selectively expressed on HLA‐A2 and HLA‐A1 phytohaemagglutinin (PHA)‐activated lymphocytes, immunoprecipitated only molecules with the same isoelectric point as HLA‐A1 and A2 products. This result suggests that the selected alloantisera do not react with the products of an HLA class I locus different from ABC but probably recognize a new epitope arising on HLA‐A molecules upon conformational changes consequent to cell

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00893.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A. Read:Medical Genetics: An Illustrated Outline.

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00894.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00895.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1990.tb00896.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY