ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00937.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYAbnormalities of the proportions of peripheral blood lymphocyte subpopulations and of immunoglobulin serum levels were found in twenty patients affected by Turner's syndrome. A slight but significantly decreased percentage of circulating T and B cells, an increased percentage of null cells and a decreasedin vitro, responsiveness of lymphocytes to phytohaemagglutinin, concanavalin A and pokeweed mitogen were found in Turner's syndrome patients. IgG serum level was found significantly decreased in comparison with age‐matched fifty‐seven normal males and fifty‐seven normal females and IgM serum level was intermediate between female and male values; Turner's syndrome patients with monosomy had an IgM serum concentration very close to male values. The derangement of T and B lymphocyte subpopulations, probably related to the aneuploidy, does not seem to be a severe one but it could account for the immunoglobulin abnormalities and for the association of Turner's syndrome with immunological disorders such as autoimmune diseases. The role ofXchromosome on IgM serum level is disc

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00938.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYThe genetic basis of allogeneic restriction in the transfer of delayed type hypersensitivity (DTH) to solubleListeria monocytogenesantigens (LMA) in rats was analysed using a set of congenic strains. The DTH parameter assayed was the localization of radiolabelled donor lymphoblasts in subcutaneous antigen stimulation sites in the ear. Sharing of the RT1.B region between the donor and host was essential for the transfer of DTH to LMA, which suggests that the RT1.B region codes for restriction elements controlling antigen recognition by TDTHcells. The recombinant haplotype RT1r1was exceptional in that transfer of DTH to A‐region matched recipients was, at least in part, possible. Measurement of donor lymphoblast localization in DTH sites afforded an opportunity to quantify allogeneic effects influencing localization of sensitized donor cells in DTH sites borne by alien recipients. Both RT1.B and RT1.A region differences could induce allogeneic effects, but they were an order of magnitude lower than those based on restricted recognition by donor T cell

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00939.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYErythrocytes that exhibit the rare blood group p phenotype lack the P antigen (globotetraosylceramide) and the Pkantigen (globotriaosylceramide). This phenotype is inherited as an autosomal recessive condition and the red cells of heterozygous individuals, parents and children of p persons, are serologically normal but no chemical analyses of their red cells have been reported. We have studied an unusual family in which all five children exhibit the p phenotype. In addition to the abnormalities described previously, the erythrocytes of four siblings had twice the normal concentration of lactotriaoslyceramide and lactoneotetraosylceramide. These cells also contained 3‐5 times as much sialosyllactoneotetraosylceramide and up to a two‐fold increase in GM3ganglioside. The glycolipids of the parents' erythrocytes were normal. Electrophoretic analysis of the glycoproteins of the proposita's erythrocytes revealed no abnormalities, but her erythrocyte membranes contained approximately 35% less galactosamine than normal red cells. This abnormality resulted from a marked decrease in galactosamine that was soluble in chloroformmethanol. The lipid‐extracted residue, which contained the glycoproteins, had a normal galactosamine co

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00940.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYThe identity and complete purification of mouse Thymocyte Interaction Modulation Factor (T IMF) is described. Use of silver‐stained PAGE methods shows that previous methods of purification yield preparations containing two protein or glycoprotein bands. T IMF activity from H‐2kmice can be bound to 15.5.5 monoclonal antibody columns (anti H‐2 Dk) but not to 11.4.1 columns (anti H‐2 Kk). The activity can be recovered from 15.5.5 columns and runs on PAGE as a single band at approximately 34,000 Daltons. This evidence together with previous evidence relating the activity to H‐2 D locus argues that T IMF is a soluble H‐2 D antigen fragment equivalent to a papainized H‐2 fragment. Additional evidence is presented on an improved method of assay of T IMF activity, on its inactivation by enzymes and serine‐esterase inhibitors and of its effect on syngeneic leucocytes and macrophages. It is also shown that T IMF is not appreciably

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00941.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYOne hundred and sixty‐five individuals with ragweed rhinitis and asthma were studied. The associations between the HLA system and: Ra3 skin‐test response, Ra3 RAST, Ra3/antigen E skin‐test reactivity ratio, Ra5 skin‐test response, Ra5 RAST, Ra5/antigen E skin‐test reactivity ratio, serum IgE levels, antigen E skin‐test response, and antigen E RAST were studied. In addition, the influence of the serum IgE levels of these immune parameters were evaluated. No highly significant associations

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00942.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYWe have previously shown that the antibody response to mammalian myoglobins is under genetic control. In the present study we examined antibodies to sperm‐whale, Atlantic bottlenosed dolphin, Black Sea dolphin, horse and badger myoglobins, raised in high responder strains of mice, to ascertain whether there is genetic control of antibody affinity to mammalian myoglobins. Using antisera of varying dilutions, the binding to125I‐labelled homologous myoglobins was studied by inhibition with homologous myoglobin over a wide range of inhibitor concentration in a modified Farr assay. The results indicated that there are no large differences between high responder strains of mice in the affinity of antibodies to mammalian myoglob

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00943.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYMice of independent haplotypes and several recombinant inbred strains were immunized with highly purified preparations of either the α‐chain or β‐chain subunit of human adult haemoglobin. Cells from the sensitized lymph nodes were challengedin vitrowith the appropriate subunit (or in some cases both chains) and cell proliferation assessed by3H‐thymidine incorporation. Mice of theH‐2bandH‐2dhaplotypes were high responders to α‐chain while mice of theH‐2f, H‐2j, H‐2k, H‐2r, H‐2s, H‐2u, andH‐2vhaplotypes were low responders. The low responsiveness of B10.A(4R) and B10.MBR and the high responsiveness of B10 indicated that theIrgene(s) determining responsiveness to the α‐chain subunit resides in the I‐A subregion of the mouse major histocompatibility complex. Mice of theH‐2d, H‐2f, and H‐2shaplotypes were high respoders andH‐2b, H‐2f, H‐2q, andH‐2uhaplotype mice were low responders to β‐chain.H‐2k, H‐2p, H‐2r, andH‐2vhaplotype mice were intermediated responders to β‐chain. The low responsiveness of B10.S(8R) and B10.TL and the high responsivenes of B10.S(9R) mapped theIrgene(s) to β‐chain to the I‐A subregion. Data collected from challenging high responder cells with both subunits indicated that α‐chain and β‐chain do not crossreact. These results are discussed in reference to earlier observations suggesting that the low responsiveness of some strains of mice to priming and challenging using the intact haemoglobin molecule might be due to a negative regulatory influence mediated by one of the subu

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00944.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYThe genetic control of thein vivogrowth of the Moloney virus‐induced BALB/c lymphoma YC8 was studied in F1 hybrids between BALB/c and several strains differing at the MHC and/or at the level ofnon‐H‐2genes. Parental strains of the B10 and C3Hf but not of A, BALB/c or DBA/2 backgrounds introduced a significant resistance to the growth of 102YC8 cells (a dose able to kill 100% of BALB/c mice) in semisyngeneic hosts. This resistance appeared to be due tonon‐H‐2genes although a modulation of the tumour growth by genes encoded by the MHC was also evident. The study of backcrosses between susceptible BALB/c and resistant (BALB/c x B10.BR)F1 crosses revealed that 83% of animals developed lethal tumors after injection of 102YC8 cells. This high frequency of tumour takes was not linked to genes of the MHC. Adult thymectomy plus sublethal irradiation was able to abrogate the resistance of (BALB/c x B10.BR) or (BALB/c x B10.RIII)F1 mice to YC8 growth. Since the injection of silica also impaired the resistance to YC8, we tentatively concluded that the genetic control of resistance to YC8 is mediated both by T cells and macrophage

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00945.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYNo.Gm, Am, Pi, andKmgene frequency difference has been observed between eighty‐seven Caucasin IgA nephropathies and ninety‐six controls. But a Km1 significant increase is found in patients with chronic renal failure compared with the total patients group. This data fits for a genetic predisposition factor in IgA nephropa

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb00946.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY