ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00442.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYIn a previous study, we searched for Sendai virus (SV)‐specific antibodies that were restricted in their binding by self‐major histocompatability complex (MHC) antigens. In C57BL/6 (B6;H‐2b) mice, most of the sera obtained after i.p. injections with syngeneic SV‐coated (SV+) spleen cells contained auto‐ and alloreactive lymphocytotoxic antibodies directed against H‐2 class I molecules, but no viral‐specific, MHC‐restricted antibodies.Here we report that syngeneic immunization with SV+cells regularly induced H‐2‐specific antibodies in various mouse strains. From a total of 12 strains tested, only the B10.S (H‐2s) strain appeared to be a low responder. The immune responses are of two types: (i) mice of some strains produce autoreactive antibodies and a broad variety of alloreactive antibodies; and (ii) mice of some strains produce only narrow or widely alloreactive antibodies. Because most of the strains differ only in theH‐2region, the patterns observed are regulated by the MHC. To locate the genes involved in the induction of H‐2‐specific antibodies more precisely, two B6 mutant strains, bml (Kbmutant) and bm13 (Dbmutant), were immunized with syngeneic SV+cells. The results suggest that theH‐2Dbregion plays an important role in the induction and specificity of the lymphocytotoxic H‐2 class I‐specific antibodies present in sera ofH‐2bmice after s

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00443.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYExpression of ABH, Lewis and related antigens was studied in the thymus of children of known ABO, Lewis and secretor status using a panel of specific reagents. ABH and Lewis antigens partly under control of the secretor status were expressed on the Hassals' bodies and a large fraction of the medullary epithelial cells. The sialyl‐Leaantigen was only present on some Hassals' bodies of Lewis‐positive individuals. ABH but not Lewis antigens were also present on cortical epithelial cells but this was independent of the secretor status. The X, sialyl‐X and Y antigens were only expressed on Hassals' bodies irrespective of the ABO, Lewis or secretor phenotype. Furthermore, the anti‐X and sialyl‐X antibodies labelled a subset of leucocytes of all the individuals tested. These results show that the genetic control of the expression of ABH and Lewis glycosidic tissue alloantigens in the thymus is different on cortical and medullary epithelial cells and stress the heterogeneity of the thymus epithel

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00444.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe bifunctional cross‐linking reagent dithiobis (succinimidyl propionate) (DSP) was used to cross‐link125I surface‐labelled glycoproteins from viable thymocytes. The cells were solubilized, and the cross‐linked material immunoprecipitated and analysed by SDS‐PAGE. When DSP cross‐linked thymocyte material was immunoprecipitated with either anti‐ThB or anti‐Ly 5 monoclonal antibodies, and then cleaved, molecules with masses identical to Ly 5 (Mr180 kD) and ThB (Mr16–18 kD) were obtained. However, if the cross‐linker was not cleaved, the intact product had a molecular mass of>200 kD. The identity of these co‐precipitated, cross‐linked moieties was formally proved by limited proteolysis peptide map analysis. The data indicated that the ThB and Ly 5 antigens were associated on the thymocyte cell surface but no such association could be found on peripheral lymphocytes. The ThB‐Ly 5 interaction may indicate an association relevant to the diff

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00445.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYTumorigenic and metastatic potential were studied in relation to class I MHC expression in four different SV40‐transformed BALB/c cell lines. All the lines studied, tumorigenic or not, expressed both H‐2Kdand Dd, so MHC antigens did not seem to be involved in the control of SV40‐transformed cells' growthin vivo. Lung metastases were observed in all tumour‐bearing mice. Cells cultured afterin vivopassage, obtained either from tumour tissue or from individual lung metastases, still expressed similar levels of H‐2dantigens, thus suggesting that tumour growth and metastasis do not occur through the selection of variants with altered MHC e

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00446.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYABH and related antigens appeared a long time ago in the evolution of vertebrates on tissues in contact with the external environment, which suggests that the polymorphism given by these antigens might play a role in the relationships of the species with pathogens. However, they are also oncodevelopmental markers and some recent experimental data suggest that they might play a role in cell‐cell recognition at some stages of development. This type of function is difficult to reconcile with the polymorphic nature of these markers unless one considers that the glycosyltransferases necessary for the synthesis of the active structures are encoded by various members of multigene families. Some non‐polymorphic members of the families would have their expression limited in time and space during development, leading to the same antigenic patterns in every individual, and these could reappear in some tumours, while the expression of other polymorphic members (A/B/O, H/h, Se/se, Le/le), leading to a variety of antigenic phenotypes, would be expressed at later stages and remain so during the whole life of the individual. The corresponding antigens could disappear from some cancer cells. It is argued that the ABH and related antigens would have primarily been involved in cell‐cell recognition phenomena. The polymorphism would have evolved later from gene duplication under environmental pressure, the expression on erythrocytes which occurred very late in evolutionary time probably being of very little biological signifi

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00447.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYSeveral class II α and β chain genes of the rabbit MHC have been cloned and classified into three distinct subregions,R‐DP, R‐DQandR‐DR, based on their homology to the corresponding HLA‐DP, ‐DQ and ‐DR genes. The organization of the rabbit MHC class II genes has now been studied in greater detail by analysing genomic DNA of an inbred III/J strain and several otherRLA‐Dhomozygous rabbits, with DNA probes derived from cloned R‐DRβ genes. Eight previously cloned R‐DRβ genes were shown to be allelic forms of fiveR‐DRβ loci. Genomic blot analyses of DNA from seven rabbits homozygous for differentRLAhaplotypes revealed that the germline contains a total of approximately seven class II β genes, one DQβ, one DPβ and five DRβ. Extensive allelic polymorphism was identified by RFLP analysis using DQ and DR probes; limited RFLP was observed with DP probes. RFLP analyses allowed us to distinguish two haplotypes which had not been previously distinguished by MLR. Such RFLP analyses will be useful for identifying MHC ‘compatible’ rabbits for various immunobiological st

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00448.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYAssociations between the major histocompatibility complex (MHC) and reproductive performance have been reported in humans, mice, rats, pigs and chickens. Only theAlocus of the bovine major histocompatibility complex (BOLA‐A) has been well characterized, and 42 alleles of this locus have been identified in American cattle. Four studies were conducted to examine the association between alleles of theBoLA‐Alocus and reproductive performance. Testis size, which is an indicator of early puberty and increased fertility in young bulls, was examined in 440 yearling bulls from nine breeds with a gene substitution model that included the effects of breed, sire, age of dam and age or weight of the bull. Estimated breeding value for twinning was examined with a gene substitution model with 204 cattle from a herd with a high frequency of twinning. Fertility of potential partners havingBOLA‐Alocus alleles in common was examined in a prospective study involving 101 pure‐bred Hereford cows mated by artificial insemination to four pure‐bred Hereford bulls. The effect of homozygosity on birth weight, pre‐weaning weight gain and post‐weaning weight gain was estimated in a sample of 683 calves from nine breeds; 22% of the calves were apparently homozygous and 78% were heterozygous at theBOLA‐Alocus. There were significant and large effects of someBOLA‐Alocus alleles on paired testicular volume, but the analyses on the other traits did not show significant associations. Substitution of the W6.1 allele for the W9A allele reduced paired testicular volume by 150&44 cm3. The W6.1 allele has now been shown to influence a reproductive trait, a production trait and susceptibility to an economically important disease. Selection for these traits may influence the frequency of the large number of alleles

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00449.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY