摘要:

SummaryImmunoglobulin was obtained from a hybridoma cell line, which was a reclone of a hybrid between a rabbit cell and mouse myeloma cell (X63‐Ag8). The immunoglobulin, isolated from the cell culture medium was found to be homogeneous by isoelectrofocusing and immunoelectrophoresis and consisted of mouse heavy and rabbit light chains, linked by disulphide bonds. All immunoglobulin molecules carried both mouse and rabbit determinants; mouse determinants were associated only with the heavy chains while rabbit determinants were only associated with the light chains. The rabbit light chains were of Ab4 allotypical specificity, but possess only some of the Ab4 determinants normally present inAb4/Ab4animals. It was sugested that the restriction in allotypical specificity may be a general property of light chainAballotypes; the normal serum immunoglobulins may be heterogeneous with respect to the allotypic determinants and any one molecule may possess only a proportion of determinants detected by a conventional anti‐allotype antise

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00729.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryImmunoglobulin allotypes were studied in a group of Iraqi Arabs. Results showed that the incidence of the Gm and Km markers investigated fall into the ranges quoted for Caucasian populations, except for the G3m(t) factor which was shown to have an incidence higher than that found in Caucasian groups.

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00730.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Summaryα‐Galactosyltransferase which participates in the biosynthesis of blood group B substance was found in urine from group B and AB healthy persons of both secretors and non‐secretors.The activity of α‐galactosyltransferase in the urine of healthy variant Bmperson was lower than that found in a normal group B person. This enzyme in urine of A1Bmpersons must be much lower than that in normal AB persons. Its activity was not detected by the method of group O to group B transformation of eryth

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00731.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryIn a family study of coeliac disease, HLA types in fifty‐three patients and their relatives were examined. There are no differences in HLA frequencies between child and adult patients. Comparison with a random series of normal controls shows increases frequencies in patients of HLA‐A1 and B8, while the family material shows that there is also an excess of haplotypeI‐8. The excess of homozygotes is thought not to be a factor in the aetiology. Intrafamilial analysis shows that only B8 is significantly associated with the disorder. It is argued that the HLA association does not indicate a ‘coeliac gene’ but that theB8allele is a major gene in a polygenic system affecting the

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00732.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummarySeveral interesting and clinically important strains ofMus musculuswere serotyped with alloantisera recognizing the different alleles of theThy‐1, Ly‐1, Ly‐2, Ly‐3, Ly‐4, Ly‐5, Ly‐6andLy‐7loci. The strains were the Biozzi high and low responder strains, of importance in immune responsiveness studies, and the strains BXSB, MRL and MRL—lpr/lpr—strains which spontaneously develop immune complex disease. In addition the related speciesMus musculus castaneuswas typed with the same reagents. The knowledge of the cell surface phenotype should prove useful in various functional studies involving these

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00733.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryMouse strains differ widely in their natural killer(NK)‐cell activity. In the (A X B6) X A backcross, high reactivity was linked toH‐2b, although non‐H‐2‐linked genes were also demonstrated (Petranyiet al., 1975). Harmonet al. (1977) demonstrated anH‐2Dd‐associated reactivity gene (1977). In the present study, we have tested eleven B10 congenic strains for NK activity. The H‐2Ddstrains B10.A, B10.T(6R), B10.S(7R), B10.HTT and B10.D2 were more highly reactive than B10, B10.S, B10.G, B10.A(2R) and B10.BR, which do not carry thedallele at the H‐2D locus. While this confirms the H‐2Ddassociation of a reactivity gene, an exception was found in the B10.A(5R) strain that was low reactive in spite of the fact that it carries H‐2Dd. This suggests the possibility that theH‐2Dd‐associated gene is outside H‐2, to the right of Tla.The AKR.H‐2bcongenic line had the same low activity as the AKR.H‐2kstrain; both were much lower than B6. This suggests either one of two possibilities: theH‐2b‐linked reactivity gene is relatively distant from the H‐2 complex, although localized on chromosome 17 or alternatively, if localized within or in the close neighbourhood of H‐2, it requires non‐H‐2genes for full expression.Previously, we have shown that the B6 X DBA/2 F1hybrid was more highly reactive than either one of its two parental strains (Kleinet al., 1978). A similar complementation effect is described in the present paper for the B10.D2 congenic strain. The high reactivity of this line can be due to the combined effect of anH‐2b‐linked gene from DBA/2 and the non‐H‐2 background of B10 or, alternatively, the former, together with anH‐2b‐linked gene from

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00734.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryCBA/N mice have an X‐linked B cell defect which prevents them from responding to non‐mitogenic thymic independent (TI‐II) antigens such as dinitrophenylated (DNP‐AGG) Ficoll. The F1male progeny of CBA/N female mice express the same defect. Spleen cell suspensions from such defective mice (CBA/N X C3H/HeN F1males) could not respond to DNP‐AGG‐Ficoll followingin vitroimmunization and subsequent transfer into irradiated, syngeneic, F1male recipients as expected. In contrast, normal CBA/N X C3H/HeN F1female spleen cells could respond and effect a ‘rescue'; they mounted strong plaque‐foriming cell 7 days afterin vitroexposure to DNP‐AGG‐Ficoll and subsequent transfer into irradiated F1male recipients. Defective F1male spleen cells could bind significant quantities of DNP‐AGG‐Ficoll, however, after,in vitroexposure. Extensive washing of these spleen cells could not reverse this binding. Such DNP‐AGG‐Ficoll‐exposed and washed F1male spleen cells could, after transfer, aid normal untreated F1female cells in their rescue function. The defective F1male spleen cells could convey immunogenic quantities of DNP‐AGG‐Ficoll to the ‘rescuing’ F1female cells.Mitomycin treatment of F1male cells did not interfere with their conveyor function. Goat anti‐mouse μ serum impeded the passive antigen conveyor function of defective F1male cells as did prior exposure to high concentrations of free DNP‐AGG hapten. Our data support the view that the B cell defect of CBA/N X C3H/HeN F1male mice does not relate to antigen binding, but rather to an inability to be effectively triggere

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00735.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryFour different C57BL/6 mutants, each with a mutation presumably in the H‐2Kblocus, were studied to determine whether the mutations had affectedIrgene function. When tested with the antigens, (TG)‐A‐L, ovalbumin (OA), and lactic dehydrogenase (LDH8), there was no difference in the antibody response of the mutants when compared to the parental C57BL/6 strain and there was also no difference in the response to the histocompatibility H‐Y antigen. The responses to these antigens have been mapped, wholly or partially in the K‐IA, IA or IB subregions and therefore the identical response of parent and mutants may provisionally exclude H‐2Kbas a site ofIrgene function for the four antigens tested. In addition, the BALB/c‐dm2mutant, a mutation in the H‐2Ldlocus, gave identical responses to the parental

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00736.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryMorphological studies of metaphase chromosomes were done with rats derived from the BIL/1 strain, which has genes affecting growth and reproduction linked to the major histocompatibility complex by conventional Giemsa‐trypsin staining and the results were compared to rat strains not carrying these defects. A subterminal‐terminal centromeric polymorphism was detected in chromosome 3 [del 3 (pter → cent)] among the strains studied. Comparison of the G‐banded karotypes of the rats carrying the defects with the karotypes of the otsher strains did not reveal any gross chromosomal abnorm

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00737.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY