ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1996.tb00270.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1996.tb00271.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SUMMARYPCR‐RFLP typing methods for DQA1 and DQB1 in conjunction with the analysis of heteroduplex and homoduplex patterns have allowed a simple method for typing all of the major DQA1 and DQB1 alleles. This method has advantages over PCR amplification with sequence‐specific primers (PCR‐SSP), PCR hybridization with sequence‐specific oligonucleotide probes (PCR‐SSO) and other PCR‐RFLP strategies for typing DQ alleles. The analysis of heteroduplex and homoduplex patterns can be used in conjunction with other PCR typing systems such as PCR‐SSP as a confirmatory step with little additional work. In addition, a PCR‐RFLP strategy was designed for resolving the DQB 1*0602 and DQB 1 *0603 alleles, which involved the use of a primer containing a base mutation, creating a new restriction site which distinguished the two alleles. These techniques have enabled resolution of the major homozygous and heterozygous combinations of these DQA1 and DQB I alleles. The PCR‐RFLP technique does not require the large number of oligonucleotides that are necessary for both the PCR‐SSP and PCR‐SSO techniques and is thus both time and cost effective for infrequent or sma

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1996.tb00272.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SUMMARYTo investigate the role of class lb genes in theQregion of the mouse major histocompatibility complex (MHC), the developmental expression and foetal tissue distribution of theQgenes were studied in the C57BL/6 mouse. Using RNase protection assays, we examinedQgene expression in a wide spectrum of foetal tissues at different stages of gestation.Q4, Q6andQ8were widely expressed in a variety of tissues. In contrast, the expression ofQ1was restricted to the thymus and intestinal epithelium.

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1996.tb00273.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SUMMARYHLA‐DR4 can be subdivided serologically into two specificities, DR4.1 and DR4.2, using well‐defined monospecific alloantisera used in the 11th International Histocompatibility Workshop. In this study, a total of 1095 random DR4‐positive individuals from several ethnic groups were tested first for serotype DR4.1/4.2 and then for DRB1 *04 alleles using polymerase chain reaction (PCR) followed by sequence‐specific oligonucleotide probe hybridization (SSOPH). An almost 100% correlation between samples testing positive for DR4.1 and the presence of alanine at position 74 was observed, while samples testing positive for DR4.2 correlated with the presence of glutamic acid at position 74. DRB 1*04 alleles 0401, 0402, 0404, 0405, 0408, 0409 and 0410 are aligned in functional groups which coincide with the serological subtype of DR4.1. DRB 1*04 alleles 0403, 0406, 0407 and 0411 coincide with subtype DR4.2. Amino acid substitutions at positions 57, 71 and 86 indicate other significant variations between alleles within the serological subgroup of DR4.1 and define five minor subgroups. The serologic and oligonucleotide allelic subgroups are in turn correlated with recognized cellular Dw antigens. While sequence data provide evidence of structural differences, data on cellular antigens support a functional association between these designated groups and their significance in transplantation and GVHD. Testing results are categorized by ethnic group in order to establish frequency data for donor selection c

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1996.tb00274.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SUMMARYThe aim of this study was to determine immunogenetic markers of susceptibility in Crohn's disease (CD), taking the different features of the clinical course of the disease into account. HLA class I, HLA class II and TAP transporter gene polymorphisms were studied using DNA typing methods. Gene and antigen frequencies were analysed and compared in a group of 102 CD patients and 200 unrelated healthy controls from the same area. Analysis of the whole CD patient population revealed no definite association with either HLA or TAP gene alleles, with the exception of an association with DRB1*1302 (Pc<0.05). However, when clinical subgroups of patients were considered, specific associations with some genetic markers were found. The most definitive results involved a genetic association in the group of patients who did not respond to glucocorticoid therapy. This group was characterized by a high frequency of HLA‐DRB1*04 (P<0.05). Conversely, a positive association with the TAP2‐A allele was found in cortico‐responder patients (Pc<0.03). Furthermore, analysis of the distribution of HLA class II alleles in relation to the presence of extra‐intestinal manifestations revealed an association with the DQB 1*0501 or *0503 suballele of DQ5 (P<0.05). Finally, patients with lesions in the small bowel were more frequently HLA DRB1 *07 (P<0.05). The present study supports the concept of clinical heterogeneity in Crohn's disease associated with a background of genetic hetero

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1996.tb00275.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SUMMARYThe polymorphism of the HLA‐G gene can be identified by PCR‐RFLP analysis. This short communication describes the PCR‐RFLP analysis of HLA‐G polymorphisms in exons 2 and 3 and the association of different HLA‐G and HLA‐A alleles in 26 healthy Finni

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1996.tb00276.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1996.tb00277.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1996.tb00278.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1996.tb00279.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY