摘要:

SUMMARYHLA antigens of the Kuwaiti population are not as well characterized as those of other international ethnic groups. We present results for HLA typing of Kuwaiti individuals using commercial Biotest sera. Six hundred and seventy one Kuwaiti were typed for HLA‐A, ‐B, ‐C antigens and 399 were typed for HLA‐DR, ‐DQ antigens. Antigen frequency and gene frequency were computed for each phenotype observed. The antigens with the highest frequencies were HLA‐A2, A1 and A3; B5, B12 and B7; Cw″4 and Cw‐l; DR52, DR5, DR3 and DR7; DQ1 and DQ2. HLA haplotypes with strong linkage disequilibrium and characteristic of Kuwaiti population are A2‐B5, A1‐B8, B7‐DR7 and B8‐DR3. A comparison study with other p

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1994.tb00200.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SUMMARYThe exceptionally strong independent association found between Lp(a) lipoprotein [Lp(a)] levels and atherosclerotic disorders indicate that Lp(a) is a factor of considerable importance in the pathogenesis of atherosclerosis. The association between Lp(a) and diabetes, rheumatoid arthritis and renal diseases suggest that Lp(a) may be involved in immunological mechanisms.Lp(a) has a great tendency to aggregate and bind to glucosaminoglycans, fibrin and fibronectin and is preferentially retained in the extracellular matrix during development of atherosclerosis and isin vitrophagocytosed by macrophages, probably as small aggregates. It was previously found that the Lp(a) level is significantly related to the HLA class II genotype in male patients with early coronary artery disease. In this paper additional results of interleukin determinations in relation to HLA type and Lp(a) levels are presented and discussed. It is suggested that an autoimmune process, perhaps triggered by a concomitant intracellular infection may occur, especially in patients with inherited high Lp(a) levels in combination with certain inherited HLA class II genotypes.

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1994.tb00201.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SUMMARYThe present paper demonstrates the usefulness of the temperature gradient gel electrophoresis (TGGE) as a precise method for the rapid identification of HLA‐DR full matched donors by molecular crossmatch analysis. The resulting TGGE fingerprints are highly diverse for different HLA‐DR genotypes and identical for samples with identical HLA‐DR type, as shown for a panel of 25 randomly selected PCR‐SSO pretyped DNA samples. In addition, TGGE analysis of a panel of homozygous typing cell lines (HTCs) established, that even subtle differences in the DR4 subtypes are detectable by TGGE crossmatch analysis. Furthermore, TGGE crossmatch analysis of unrelated donor/patient pairs demonstrated, that only HLA‐DR identical patient/donor combinations showed identical TGGE patterns. Thus crossmatch analysis by TGGE presents a novel basis for a rapid and safe unrelated bone marrow donor selection strategy. Only HLA class I identical donors which also show HLA‐DR identity in the TGGE crossmatch test are selected for the final confirmation of the HLA class II genes by a suitable DNA subty

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1994.tb00202.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SUMMARYThe Rodgers (Rg) and Chido (Ch) blood groups are antigenic determinants of the fourth component of human complement C4. They are associated with the two isotypes of C4, C4A and C4B, respectively. They serve as markers to distinguish C4A from C4B as well as for the definition of subtypes of common and rare allotypes. As an alternative to the serological typing method using human alloantisera, a PCR typing procedure with sequence‐specific primers (PCR‐SSP) was designed. The method was tested on selected DNA samples from individuals with well‐defined C4 allotypes. No false‐positive or false‐negative typing results were obtained and all the determinant combinations could be distinguished. The PCR genotyping allowed the detection of all Rg/Ch sequence determinants of each isotype. Thus, reverse antigenicity could also be established in the presence of other C4 allotypes without a segregation study. To exclude the possibility that PCR‐typed determinants originate from a non‐expressed C4 null gene, a sequence‐specific PCR was established detecting a 2‐bp insertion in exon 29 described previously as a cause for C4A non‐expression. PCR Rg/Ch genotyping provides a fast and efficient method for routine typing in HLA haplotype and disease

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1994.tb00203.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SUMMARYHLA‐B14 positive haplotypes have increased frequencies in a group of patients with puberty disorders, IgA deficiency and cancer of the ovary. Clinical investigations demonstrated that all these patients have high values of 170H progesteron after the ACTH test which suggests an alterated function of 21 hydroxylase enzyme. In order to investigate whether these B14 positive haplotypes carry the same CYP21 mutation in the various diseases and controls, we have amplified by polymerase chain reaction (PCR) the sections of CYP21B gene which include amino acid positions 172 and 281 where typical mutations are known to occur in 21 hydroxylase deficiency. The presence or absence of the defined mutations was tested by oligonucleotide hybridization using oligonucleotides, labelled with DIG‐ddUTP, designed to hybridize with the mutated or with the normal sequence. It was found that regardless of whether the subject tested was a patient or a healthy control the mutation at position 281 was found in all cases carrying HLA‐B14, DR1 haplotype. Interestingly, this mutation does not seem to be in association with HLA‐B14, DR7 haplotype.These findings suggest that CYP21 gene plays a role in all these differing diseases although it must be stressed that there may be alternative explanations for the observ

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1994.tb00204.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SUMMARYDPB1 locus typing of the 155 cell 4AOHW panel was performed using a PCR‐RFLP method. Ambiguity of allele assignment was resolved by amplification using sequence‐specific primers. Of the 150 cells for which typings were achieved, three exhibited unusual restriction enzyme fragment patterns, suggesting the possibility of novel DPB1 alleles. Sequence analysis revealed one allele present in the currently reported 46, one novel allele (4AOHW/107) not present among the 46, and one from a non‐human primate which is being investigated. Twenty‐six (26) of the 34 10IHW cells have been studied previously by cDNA RFLP, and strong haplotypic associations have been demonstrated between DPA1 and DPB1 locus alleles. It is proposed that exploitation of intron polymorphisms marking haplotypes will be an integral part of future DPB 1 typing as a ‘first‐pass’ stratification process to minimize the requirement for sequence‐based methods to definitively assig

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1994.tb00205.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SUMMARYWe report the case of an Indonesian patient who required urgent bone marrow transplantation for acute leukaemia and who received successive transplants from two siblings. The first transplant failed while the second was successful. There were some uncertainties in serological typing due to the presence of cross‐reacting HLA‐B alleles, lack of paternal typing and the use of Caucasoid sera for Indonesian patients. Distinction between the two donors was also difficult. Interestingly, the use of a new DNA technique identified the presence of differences between the patient and the first unsuccessful donor but not the second successful do

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1994.tb00206.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SUMMARYPatients with CAH, extrahepatic HBV manifestation and healthy children were studied for presence of Gm 1,2,3,10,21 factors and Km 1 factor. Significantly higher frequency of Gm (1, 2, 3, 10, 21) phenotype was shown in CAH group as compared with the other two groups. Relationship between Km factors and examined groups was not found.

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1994.tb00207.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SUMMARYWe evaluated the TNF‐ß gene polymorphism in two HLA‐associated autoimmune diseases, multiple sclerosis (MS) and rheumatoid arthritis (RA). The TNF‐β allele and genotype frequencies were not significantly different in the patient populations, compared to controls. An increased frequency of the TNF‐β* 2 allele was observed in HLA‐DR2+vs. HLA‐DR2‐MS patients. No such association was seen in HLA

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1994.tb00208.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1994.tb00209.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY