摘要:

SUMMARYIn the course of raising monoclonal antibodies to human colonic and small intestinal epithelium, three were isolated which recognized epitopes on many colonic glycoproteins and many jejunal glycoproteins taken from some individuals, but not on the same glycoproteins from others. The specificities of these antibodies were examined by carrying out inhibitions of the binding of the antibodies to the jejunal glycoproteins by blood group substances and purified oligosaccharides. The glycoproteins were separated by electrophoresis and transferred to nitrocellulose strips which were then used for the inhibition experiments. The results indicated that the antibodies bind to Lea‐active structures. The conditions were determined for achieving red cell agglutination by two of the antibodie

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00462.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYmAb KUL/05, a novel murine monoclonal antibody, reacts with molecules displaying the typical tissue distribution and molecular profile of class II MHC antigens. An extensive scrutiny employing serological and immunochemical assays on DR homozygous and DRα‐ mutant cell lines has shown that this reagent displays some additional, interesting features, namely mAb KUL/05 (a) binds in a broadly monomorphic fashion to cells of DR1 through seven specifities, (b) recognizes a determinant shared by a large proportion of DR, DQ and DPβ chains from most haplotypes, in both their monomeric and α chain‐associated forms, and (c) reacts with frozen, acetone‐fixed, as well as conventional, formalin‐fixed, paraffin embedded tissues. Thus, mAb KUL/05 is likely to represent a useful adjunct for the study of the expression of class II MHC products in normal and pathological tissue

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00463.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYSubstrains of NZB mice have been compared by Southern blot analysis using several probes. The restriction fragment length polymorphism of probes derived from the Igh‐V, Igk‐V, Tcrα‐C loci and of the long terminal repeat of the mouse mammary tumour virus revealed that NZB/BlLwPtIbm were grossly different from NZB/BlNJ and NZB/BlOla. Comparison with mouse strains of the Igk‐V haplotypes a and d suggested that NZB/BiLwPtIbm contain genetic material of the C58 mous

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00464.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYCBA/J (H‐2k) females, mated with DBA/2 J (H‐2d) or DBA/1 J (H‐2q) males, exhibit a high rate of fetal resorption. In contrast, when H‐2 identical CBA substrains (i.e. CBA/Ca and CBA/N) are used, this phenomenon is not observed. On the other hand, before mating with DBA/2 J males, pre‐immunization of CBA/J females with spleen cells coming from BALB/c J or (DBA/2 x BALB/c J) F1 males (and not from other H‐2didentical males whatever their Mls alleles) has significantly decreased the fetal resorption rate. Thus, immunization against determinants other than classical H‐2d(K, I, D, L) antigens (transmitted as a dominant character and different from Mls determinants) can elicit anti‐abortive effects. Furthermore, it was observed that the spleen cell endowed with the anti‐abortive effects was neither a T nor a B lymphocyte. In contrast, peritoneal cells were able to reproduce the phenomenon, indicating that it may be mediated by a cell of the macrophage‐monocyte lineage. Finally, a first gestation was substituted for allo‐immunization of CBA/J females. The anti‐abortive effects of a first pregnancy by BALB/c J males (and not by other H‐2ksyngeneic or H‐2dallogeneic males) was observed in the course of a second pregnancy sired by DBA/2 J males. These data can be interpreted in terms of maternal recognition of an antigen present on both macrophages and trophoblast cells and necessary for a successful gestation, which is coded for by genes out

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00465.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe products of the major histocompatibility complex (MHC) loci regulate an individual's immune response to pathogens. Cattle provide an important model to study the relationship between disease susceptibility and MHC haplotype since large half‐sibling families are common. The definitive demonstration, however, of a firm relationship between MHC phenotype and disease susceptibility in cattle will require a precise definition of the bovine MHC allelic products. Available reagents for serological characterization of the bovine MHC gene products have not been adequate for these purposes. We have shown that existing mouse monoclonal antibodies and rabbit anti‐human antisera precipitate bovine class I molecules, that these structures separate well by one‐dimensional isoelectric focusing (1‐DIEF), and that immunoprecipitation followed by 1‐DIEF allows the detection of bovine class I MHC allelic products. Through this technique, we have identified previously undetected class I products. This approach will facilitate a detailed characterization of the bovine MHC class I gene

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00466.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYKaposi's sarcoma is associated with an increased frequency of HLA‐DR5. The hypothesized model of a susceptibility gene in linkage disequilibrium with DR5 may be tested by haplotype analysis in familial Kaposi's sarcoma. Our finding of no common haplotype among afflicted members of a family provides evidence against the hypothesized linkag

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00467.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

R. H. Michell, A. H. Drummond andC. P. Downes(eds).Inositol Lipids in Cell Signalling.J. Gergely:Multifunctional IgG and IgG‐Binding Receptor

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00468.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00469.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY