摘要:

SUMMARYThe genetic determinants responsible for the specificities of somatic antigens ofEscherichia coli2B‐V, O6and O86, which also have blood group H, A and B activity, respectively, are located near the his locus on chromosome

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1975.tb00524.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SummaryWith an improved method for extraction of HL‐A soluble antigen from dried blood stains, eighteen HL‐A antigens were tested in the microcytotoxicity test as described in the preceding paper (Rittner&Waiyawuth, 1974). With the antisera available, seven antigens could not be detected with sufficient confidence. As a serious handicap of the microcytotoxicity test, a selected panel of donor lymphocytes must be permanently available. A comparison between the microcytotoxicity and the microcomplement fixation test performed on platelet material recovered from the stains led to the conclusion that the microcomplement fixation test is better suited for a routine laboratory. Encouraging results were obtained in a larger series of microcomplement fixation tests with white blood cells from dried blood stains. Significant chi‐squared values were calculated for fourteen of sixteen antigens tested. The possible causes of false positive and negative reactions are discussed. Anticomplementary reaction of anti‐HL‐A sera in microcomplement fixation tests should be taken int

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1975.tb00525.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYThe immunogenicity of Thy‐1 antigens was examined using the Thy‐1 congenic mouse strains A and A/Thy‐1.1. A disequilibrium of response was found between these strains. A strain mice rejected skin grafts and produced high titres of antibody to lymphoid cells from A/Thy‐1.1 strain mice. However, in the reverse combination grafts were not rejected nor was there any significant antibody response. A/Thy‐1.1 mice did not respond to Thy‐1.2 antigen when it was presented on an H‐2 allogeneic background (A·SW strain) but did respond to it when non‐H‐2 incompatibilities were involved (B10·A strain donor). We postulate the existence ofIrgene(s) for the response to Thy‐1.2 antigens which are linked to H‐2. The failure of A/Thy‐1.1 strain mice to respond to Thy‐1.2 antigens in the absence of carrier determinants is the result of a deletion (in the germ line) of the appropriateIrgene itself resulting from the long association of the H‐2ahaplotype of the A mouse (donor of the genetic background of A/Thy‐1

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1975.tb00526.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYIn a cell free system derived from peripheral human leucocytes an informative RNA (i‐RNA) was synthesized after antigenic stimulation with benzoyl‐penicilloyl‐formyl‐l‐lysine (BPoFLys). This i‐RNA was used to synthesize antibody in a second cell free system. The ribosomes used in the antibody synthesizing system were derived from peripheral human leucocytes, from human lymphoblasts CCRF and from HeLa cells. Furthermore, hybrid recombined ribosomes from 60S HeLa‐subunits and 45 S CCRF‐subunits as well as from 45S HeLa‐ and 60S CCRF‐subunits were used to synthesize antibody. Only ribosomes from peripheral human leucocytes, from CCRF‐cells and hybrid recombined ribosomes from 60S HeLa‐ and 45S CCRF‐subunits were able to synthesize antibody in the cell free system. Antibody synthesized in the cell free system was detected by phage neutralization test and by equilibrium dialysis. By these methods new markers could be demonstrated which are essential for the differentiation to an a

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1975.tb00527.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYIn continuation of earlier investigations the determinant structure of human fibrin‐stabilizing factor (factor XIII) was analysed by comparative determinant analysis (CDA). Two determinants, P1and A were distinguished; P1is shared with all subhuman primates studied, while it is missing on all non‐primate homologues. A is found on both primate and non‐primate homologues. The possible presence of an additional network determinant is discussed. There is no human species‐specific dete

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1975.tb00528.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYIt is shown that S‐s‐U‐erythrocytes have no detectable PAS‐3, B‐ and C‐glyco‐protein bands which are demonstrable by SDS‐polyacrylamide gel electrophoresis of red cell membranes and subsequent PAS‐staining. PAS‐1 and ‐2 are normal with respect to electrophoretic mobility and staining intensity. The staining intensity of PAS‐3, B and C from heterozygous defective erythrocytes is about half of normal. One S‐s‐U+ blood sample did not d

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1975.tb00529.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Book Review in this ArtileElectrophoresis and Isoelectric Focusing in Polyacrylamide Gel, Edited by R. C. Allen, Walter de GruyterCurrent Topics in Microbiology and Immunology, Edited by W. Arber, R. Haas, W. Henle, P. H. Hofschneider, J. H. Humphrey, N. K. Jerne, P. Koldovsk, H. Koprowski, O. Maaløe, R. Rott, H. G. Schweiger, M. Sela, L. Syruček, P. K. Vogt, E. WeckerCurrent Topics in Microbiology and Immunology, Edited by W. Arber, R. Haas, W. Henle, P. H. Hofschneider, J. H. Humphrey, N. K. Jerne, P. Koldovsk, H. Koprowski, O. Maaløe, R. Rott, H. G. Schweiger, M. Sela, L. Syruček, P. K. Vogt, E. WeckerMolecular Population Genetics and Evolution, M. Nei (Frontiers of Biology, Vol. 40, Editors A. Neuberger, E. L. Ta

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1975.tb00530.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1975.tb00531.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY