摘要:

SummaryAn investigation of the serological and biochemical properties of red cells in two unrelated British families revealed the probable presence of examples of the rare genotype EnαEn.In one family the En‐modified red cells carried N‐like determinants associated with s. In the other family M‐like determinants associated with S were

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00340.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryThe effects of thymocyte interaction modulation factor on the adhesion of mouse allogeneic thymocytes and B‐cells are reported. This glycoprotein, produced by short term cultures of thymocytes, has already been described as reducing the adhesion of syngeneic B‐cells, leucocytes and macrophages. Adhesion was measured in suspension culture using the collision efficiency method. This paper reports that:1. In addition to the syngeneic effect of thymocyte IMF in reducing adhesion of certain unlike cell types there is also an allogeneic effect in which an allogeneic T‐IMF will diminish the adhesion of a thymocyte population, or still further reduce the adhesion of a B‐cell population than would a syngeneic T‐IMF.2. Thymocyte IMFs were prepared from different congenic strains and tested on the adhesion of syngeneic and allogeneic thymocytes. When factor and cells were syngeneic or metched at any H‐2 locus except H‐2 D there was no effect on adhesion since it remained at the same value as in controls in their own IMF. But whenever factor and cells were mismatched at H‐2 D there was a marked diminution in the adhesion of the cells.3. Antibodies raised against specific thymocyte IMFs could be used to detect the presence of T‐IMF binding to the surface of cells by immunofluorescence or immune cytolysis. These systems show that the antibodies against thymocyte IMF can be used to type the H‐2 D type of a cell and that these factors are present at the surface of thymocytes and certain other cell types. They confirm that the thymocyte IMF is either in H‐2 D product or is closely associated with H‐2 D locus in its binding and action. They also show that the T‐IMF antigen on non‐lymphocytic types is produced by T‐cells or thymocytes.4. The general relevance of these results is discussed in relation

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00341.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryMice of two congenic inbred strains C3H/Sn (H‐2k) and C3H. B10 (H‐2bwere infected with 100Schistosoma mansonicercariae. After the infection, the following parameters for the immunologica response were studied: worm burden, mortality, antibody titre, spleen index, eosinophilia, delayed type of hypersensitivity andin vitroresponse to threeS. Mansoniantigen preparations.No difference in the worm burden and in thein vitroresponse to the antigen preparations of adult worm antigen, soluble egg antigens and the egg antilgen MSA1, was found. The C3H.B10 mice showed a singificantly higher mortality, antibody titre and delayed type of hyprsensitivity while the C3H/Sn mice showed a significantly higher spleen index and eosinophilia. This indicates that the H‐2 region influences the course of an acuteS. mansoniinfection, whereas the susceptibility to the infection seems not to be influenced, as is shown by the worm

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00342.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00343.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryThe lymphocytes from 107 maternal‐foetal pairs were examined for theirin vitroresponsiveness, as determined by the incorporation of tritiated thymidine following stimulation with phytohaemagglutinin (PHA), candida, varicella, mumps, streptokinase‐streptodornase (SKSD) and tetanus toxiod. The data were collected and analysed in two sequential groups (forty‐seven and sixty) in order to determine whether the results were reproducible. The variable chosen for analysis was the difference (d) between the square roots of the isotope incorporation in the stimulated and control cultures because it gave the most symmetrical distribution of the data. The experimental error in the determination of maternal lymphocyte stimulation was 1.4‐8.6% and of the and of the foetal lymphocytes, 1.0‐16.6% depending upon the antigen or mitogen and its concentration. The data in the two sets of patients were statistically the same in forty‐eight of the fifty‐six analyses (fourteen antigen or mitogen concentrations in autologous and AB plasma for maternal and foetal lymphocytes). The statistical limits of the distribution of responses for stimulation or suppression were set by an analysis of variance taking two standard deviations from the mean as the limits. When these limits were translated into stimulation indices, they varied for each antigen or mitogen and for different concentrations of the same antigen. Thus, a detailed statistical analysis of a large volume of lymphocyte transformation data indicates that the technique is reproducible and offers a reliable method for determining when significant differences from control value

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00344.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryThe response of 105 maternal‐foetal lymphocyte pairs to specific and non‐specific stimulation were evaluated using a newly defined method of analysis. There were no significant differences in the responses of maternal or foetal lymphocytes to phytohaemogglutinin (PHA) or the various antigens as a function of concentration over the ranges tested. The maternal lymphocytes were stimulated by all of the antigens and responded to PHA three‐five times more strongly than to the antigens. The foetal lymphocytes were stimulated by PHA and tetanus toxoid only and were suppressed by streptokinase‐streptodornase (SKSD). They responded to stimulation by antigens at a lower level than did the maternal lymphocytes, but they responded at a much higher level to PHA. Unstimulated cultures of foetal lymphocytes incorporated more isotope than did those of maternal lymphocytes in both autologous and AB plasma.The data were cross‐classified to determine whether the responses of the foetal lymphocytes varied concordantly with the responses of the maternal lymphocytes in both autologous and AB plasma by the Chi‐square test for independence and by rank correlation analysis. There was no significant correlaiton in either plasma to stimulation with the antigens. Thus, the presence of antigen reactive lymphocytes in the circulation of the mother does not mean that the foetus is sensitized to that antigen.Comparison of the lymphocyte responses in autologous plasma with those in AB plasma provided evidence for the presence of circulating immunoregulatory substances. Autologous maternal plasma suppressed the lymphocyte responses to high concentrations of candida and SKSD and stimulated the response to mumps, varicella and tetanus toxoid. Autologous fetal plasma suppressed the lymphocyte responses to candida, varicell and SKSD and stimulated the response to PHA. The responsiveness of maternal lymphocytes to PHA was less in foetal plasma than in autologous maternal o

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00345.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY