摘要:

SUMMARYThe second exon of the DRB1 gene encoding for the first domain of the HLA‐DR β‐chain was sequenced in 16 patients (10 DR4/DR1 positive, 6 DR4/DR1 negative) with seropositive rheumatoid arthritis (RA). We could confirm the strong association of susceptibility to RA with functionally equivalent conformations on otherwise distinct MHC molecules. At least one HLA‐DR allele in all of the analysed DR4 or DR1 positive patients showed such an epitope with a minimal variability limited to residue 71.However, in HLA‐DR4 and ‐DR1 negative patients such a similar epitope could not b

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1993.tb00096.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SUMMARYThe distribution of phenotypes and gene frequencies of the plasminogen were studied in 203 blood donors of the Han population, Chengdu, China using isoelectric focusing followed by immunofixation. The results were as follows: PLGA=92.12%, PLGAM5=4.39%, PLGAB=1.97%, PLGAB2=0.98%. Their gene frequencies were: PLG*A=0.9606, PLG*M5=0.0246, PLG*B=0.0099, PLG*B2=0.0049. This study implied that PLG*M5 might be the specific genetic marker of the Mongoloids.

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1993.tb00097.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SUMMARYThe interest in Lp(a) lipoprotein [Lp(a)] has increased dramatically during the last few years due to the documented strong association between high Lp(a) levels and early atherosclerosis and its sequelae and the probable additional thrombogenic effect of inherited high Lp(a) levels.However, some paradoxes still remain to be solved in Lp(a) research. This pilot study was performed to test whether some criteria could be found for an association between Lp(a) levels, HLA genotype, and early coronary heart disease. If so, it could help to explain why some individuals with high Lp(a) levels get atherosclerosis while others with comparable lipid levels do not. It would also indicate that immunological processes could be involved in Lp(a) associated atherosclerosis.In this case‐control study the HLA‐DR genotypes 13 or 17 were significantly more frequently encountered in 30 male patients with early coronary heart disease than in 30 sex and age matched healthy controls (P =0.012). These HLA‐DR specificities were especially frequent in male patients with high Lp(a) levels. The same HLA‐DR genotype pattern was not seen in 30 female patients, although there was a trend towards more frequent 13a g

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1993.tb00098.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SUMMARYA simple PCR‐based protocol for HLA‐DR typing suitable for a routine practice is described. The method involves, first, a PCR amplification with seven different, group‐specific (DR1, DR2, DR4, DR7, DR9, DR10, and DR3+5+6+8) primer‐pairs, and second, typing of HLA‐DR allele more exactly in DR1, DR2, DR4, and DR3+5+6+8 groups by digestion of PCR products with restriction enzymes distinguishing different HLA‐DR types within each of the groups. Altogether 24 HLA‐DR alleles, or any combination of these, can be typed. The whole procedure, starting from a blood sample, can be carried out during a single working‐day. The method was tested by typing a set of homozygous cell lines, as well as a local panel previously typed by PCR/oligotyping. Also, 227 patients waiting for transplantation were typed to test the method in a routine setting. The results suggest that this kind of approach gives reliable HLA‐DR types and works well i

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1993.tb00099.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SUMMARYIn order to produce mAbs directed specifically against HLA‐DR10 molecule, transfected mouse L cells, expressing the DRB 1*1001 allele, were used to immunize C3H mice over a period of 4 weeks. Two mAbs, 2C12 and 4B6, derived from this fusion were found to recognize, with different affinity, polymorphic epitopes of DR10 that are shared with DR1, 3, 7, and 9. These mAbs were screened on a large panel of homozygous B lymphoblastoid cell lines using microlymphocytotoxicity and the results were confirmed by flow cytometry. The reactive pattern of 2C12 and 4B6 was compared to that of MP10 human mAb also recognizing the DR10 specificity in addition to DR1, 2 and 9.Based on serologic specificity and cellular absorption experiments, we conclude that the epitopes the murine and human mAbs respectively recognize on the DR10 molecule, are probably differen

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1993.tb00100.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1993.tb00101.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Book Reviewed in this article:A.M. Lesk:Protein Architecture. A Practical Approach.G. Reeves&I. Todd:Lecture Notes on Immunology Second Edition.JamesMcCluskey(ed.):Anfigen processing and recognition.

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1993.tb00102.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1993.tb00103.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SUMMARYMouse mammary tumour viruses (Mtv) are B‐type retroviruses. These can be exogenous, transmitted via maternal milk, or endogenous, as proviral integrations into the mouse genome, transmitted vertically in a Mendelian fashion. A number of different sites of integration of endogenous Mtvs have been reported in various inbred mouse strains. An open reading frame (ORF), within the long terminal repeat (LTR) of Mtv, encodes a type 2 integral membrane glycoprotein. The ORF products are expressed in association with MHC class II molecules at the cell surface and have an affinity for certain T cell receptor (TCR) Vβ chains such that CD4+8+TCR+double positive thymocytes expressing these Vβ chains undergo programmed cell death in mice carrying the appropriate endogenous or exogenous Mtvs. This constitutes a measurable part of negative repertoire selection of the T cell repertoire. Some positive selection of the T cell repertoire also appears to be TCR Vβ‐specific, although the involvement of polymorphic ligands other than MHC molecules is not apparent. This minireview summarizes the published work on the TCR Vβ specificity and chromosomal localization of the various mouse mammary tumour proviral integrations leading to negative selection, and discusses the nature of TCR Vβ‐specific positive

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1993.tb00104.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY