摘要:

SUMMARYDifferences between the mouseLy5aandLy5balleles can be distinguished on the basis of polymerase chain reaction (PCR)‐restriction enzyme analysis and differential monoclonal antibody reactivities. To more precisely map theLy5gene on the mouse chromosome 1, analytical DNA and protein tests were performed on recombinant inbred strains of mice prepared from SJL/J (Ly5a) and B ALB/cke (Ly5b) progenitor strains. Each recombinant inbred strain was characterized to determine whether it carried theLy5aorLy5ballele. Both assays, DNA‐PCR and protein‐immunofluorescence, yielded identical results for each strain examined. Placement of theLy5gene with respect to other characterized markers of mouse chromosome 1 for these recombinant inbred mouse strains shows a gene order ofIdh‐1.Ity:Pep3:/Ly

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1991.tb00015.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SUMMARYMink (Mustela vision) class I leucocyte antigens, here abbreviated MhcMuvi according to the proposal given by Kleinet al.(1990), were characterized by isoelectric focusing (IEF), using Triton X‐114‐extracted and neuraminidase‐treated membrane proteins from spleen cells, followed by immunoblotting and development with a murine monoclonal antibody raised against denatured major histocompatibility complex (MHC) class I antigens.Muvi class I antigens were investigated in a group of 97 Danish mink (including five families), of six types (Standard, Wild, Pastel, Pearl, Violet and Sapphire), and a group of 110 French ‘Wild’ mink. For each mink type maximally six protein bands in IEF were identified. Standard, Pastel and Sapphire mink only exhibited from two to four bands. This to us indicated restricted polymorphism of the Muvi class I antigens for these mink. The restriction patterns varied from farm to farm. In the family material the Muvi antigens were found to segregate as expected.The French Wild mink were all naturally infected with Aleutian disease virus (ADV), a parvovirus, and their disease status classified as progressive or non‐progressive. There were approximately 50% in each group. The mink were grouped into one of five class I Muvi profiles. When the profiles were compared to progressive versus non‐progressive disease status, we found that mink with Muvi profile 4 and 5 almost exclusively (13 out of 15) were classified as having progressive Ale

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1991.tb00016.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SUMMARYDifferences were detected between male‐specific antigen(s) from BALB/c and C57BL/10 mice. Reciprocal crosses between these strains were analysed by means of a popliteal lymph node assay enumerating cells secreting IgM and IgG in a T‐dependent bystander B‐cell response. The responses, while being indirect, weak and variable, suggested that there are differences in ‘immunogenicity’ coded by Y‐linked (male‐specific) genes. This conclusion was strengthened by the results of experiments carried out in Y‐backcross mice, where spleen cells from male C57BL mice with a BALB/c‐Y (B10.C‐YC) stimulated low popliteal lymph node responses in female littermates in comparison with C57BL mice with their own Y‐chromosome. In contradistinction, male spleen cells from a BALB/c with a C57B1/10‐Y chromosome (C.B10‐YB), injected into a hind footpad of a female littermate, induced a relatively higher popl

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1991.tb00017.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SUMMARYPolymerase chain reaction/oligonucleotide typing was used to identify HLA‐Cw*0601 (Cw6) in patients with psoriasis and psoriatic arthritis. The assignment of HLA‐Cw*0601 was established by the concordant presence of codons for alanine (position 73), lysine (position 80) and tryptophan (position 97). The frequencies of all three codons were increased in the patient gro

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1991.tb00018.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Book Reviewed in this article:F. Shakib(ed.):The Human IgG Subclasses: Molecular Analysis of Structure, Function and Regulation.F. Brown, R. M. Chanock, H. S. Ginsberg&R. A. Lerner(eds): Vaccines 90.Modern Approaches to New Vaccines Including Prevention of AIDS.A. Meager:Cytokines.G. E. Lamming(ed.):Marshall's Physiology of Reproduction, Volume 2, Reproduction in the Male, Fourth Edition.

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1991.tb00019.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1991.tb00020.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1991.tb00021.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1991.tb00022.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1991.tb00023.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY