摘要:

SUMMARYTLX antigens have been found on most peripheral blood cells, trophoblasts, seminal vesicle cells and sperms. These antigens seem to be associated with the membrane co‐factor protein (MCP) and the CD46 antigen. Alloantibodies to TLX antigens with FctRII‐blocking features were obtained by transfusion of leucocytes or platelets. Preliminary population studies revealed that alloantibodies to TLX/CD46/MCP recognize four overlapping specificities. The terminology TLX‐B was introduced with specificities TLX‐BI, B2, B3, B4 and frequencies obtained in the population were: 38%, 46%, 42% and 26%, respectively. Family studies showed an independent segregation of the TLX and HLA alleles.At the cellular protein on trophoblast, the alloantibody detected a glycoprotein of 66‐67 kDa molecular mass, which may correspond to the a chain of the TLX/CD46/MCP isotypes. A direct association of the alloantibody with FctRII could be excluded thus its FctR blocking feature is probably based on an indirect functional effect.After transfusion and in pregnancy the induction of TLX alloantibody production depended on the mismatching in the TLX/CD46/MCP phenotypes. Probable associations were revealed in the case of recurrent habitual abortion between the lack of FCTR blocking antibody production and the matched TLX specificities of the couples. After transfusion, TLX alloantibody production with FCTR and MLR blocking function was induced only when the recipient was lacking the TLX specificities expressed on the donor cells. Suppression of MLR was found only when TLX specificity in sera corresponded to the TLX specificity of the effector cell. The immunopathological importance of these findings in transplantation and reproductive medicine has yet to be

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1995.tb00225.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SUMMARYSelective IgM deficiency is commonly found in Bloom's syndrome (BS). We reported that membrane‐bound μ (μ m) mRNA was well transcribed but secreted μ (μ s) mRNA was not, although there was no mutation or deletion in the sequence including the (is C‐terminal coding sequence in the patients with BS. Furthermore, we have shown previously, preferential damage to IgM production by ultraviolet (UV) irradiation of the cells of the patient. In the study described here, mutation in the sequence which is upstream of the 5’ end of the μ s C‐terminal coding sequence was induced by UV irradiation in the lymphoblastoid cell line (LCL) of BS patient. These results suggest that abnormal repair of DNA damage is present in this LCL, and that preferential damage to IgM production by UV irradiation in this LCL may be due to the abnormal repair o

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1995.tb00226.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SUMMARYWe studied the allelic constitution at the HLA class II DQA1, DQB1, DRB1 and DPB1 in 94 Italian multiple sclerosis (MS) patients and 98 controls. No significant increase in the frequency of DR2 alleles was detected among MS patients, as previously observed both in European and some Italian studies. A slight increase was found for the DQA 1*0301 and DQB 1*0602 alleles in the MS patients. No significant association was found with the glutamine residue at position 34 of the DQ α chain, which was noted previously in MS patients from northern Europe

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1995.tb00227.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SUMMARYFifty‐two British and 29 Greek idiopathic membranous nephropathy (IMN) patients were analysed for DRB, DQA1, DQB1 and DPB1 gene polymorphism using second exon amplification and sequence‐specific oligonucleotides (SSO). In addition 100 British and 92 Greek controls were analysed.A highly significant increased frequency of the DRB 1*0301 allele was found in IMN patients from Britain (80%), when compared to controls (27%, OR 10.6,P= 0.000004). A lower frequency of DRB 1 *0301 was observed in Greek IMN patients (33%), but this was just significant before correction, when compared to Greek controls (15%, OR 3,P= 0.02). The DRB3 allele most often associated with DRB 1 *0301 was DRB3*0101 (OR 4.2,P= 0.00025) in British patients and DRB3*0201/2 (OR 11,P=0.006) in Greek patients. In Greek IMN patients a decrease in DR16 was found (OR 0.08,P=0.004), and the overall incidence of DR2 was significantly lowered when both sets of IMN patients were combined (OR 0.21, χ217.6,P= 0.00013).The incidence of DQA1 *0501 was raised in both Greek (96%vs.66%, OR 9.7, χ26.9,P= 0.009) and British IMN (85%vs.45%, OR 7.4, χ220,P= 0.00007) patients. This gives some support to a proposal for a major role for this allele in IMN. However, DQB1 *0201 was also raised in both Greek (50%vs.21%, OR 3.6, χ28.1,P= 0.005) and British (90%vs.44%, OR 10, χ221.7,P=0.00004) IMN patients. The DQA1*0102 allele was significantly lowered in Greek IMN patients (15%vs.32%, OR 0.05, 12.2,P=0.0008), probably reflecting a lowering in the DR16 haplotype. No significant difference was observed in the frequencies of DPB alleles in patients and controls.It is concluded that DRB 1*0301 has the strongest association with British Caucasoid IMN. The Greek Caucasoid IMN association with DRB 1*0301 is weaker, and a role for other alleles has not been el

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1995.tb00228.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SUMMARYWe have characterized TAP allele frequencies in a panel of 71 Yoruba Nigerians using ARMS‐PCR. With the exception that TAP 2D was absent in Nigerians, TAP 2 allele frequencies in this population were found to be similar to those in a UK white population. HLA‐DR4 also was found to be at a low frequency in Yoruba Nigerians (1.4%). This may reflect the absence of TAP 2D in Nigerians as DR4 and TAP 2D are in linkage disequilibrium in UK Caucasoids.The most frequent TAP 1 allele in Yoruba Nigerians was TAP 1A (49%). However, this value will be an underestimate as TAP1 alleles could not be unequivocally assigned in 41 % of subjects using the ARMS‐PCR method

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1995.tb00229.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SUMMARYMolecular, cellular and serological analysis of Major Histocompatibility Complex alleles was performed on three individuals who appeared to be HLA‐DR10 homozygous by DNA restriction fragment analysis. Each donor was of different ethnic origin: Caucasoid, Asian Indian and African Negroid.The results of our studies show that the Caucasoid and Asian donors are indeed homozygous for the HLA‐DR 10 allele, while the African donor also possesses the DRB1*0103 allele. Homozygosity for the HLA‐A1B37‐Cw6‐DR10‐DQ5 haplotype in the Caucasoid donor was confirmed by familial segregation analysis. The B‐lymphoblastoid cell line produced from this donor should prove useful in studies of HLA i

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1995.tb00230.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SUMMARYThe serologically defined canine MHC gene products (DLA‐A and DLA‐B) were investigated by one‐dimensional isoelectric focusing after detergent phase separation, and immunoblotting (1D‐IEF). The animals studied (n= 36) represented three different breeds. Since DLA‐A and DLA‐B antigens are MHC class I and class II molecules, respectively, cross‐reactive polyclonal antibodies specific for human class I or class II molecules were utilized to visualize the protein pattern. The relative positions of the bands were correlated with the DLA‐A and DLA‐B antigens. In most cases DLA antigens of identical serological type did not differ in their 1D‐IEF pattern except for DLA‐A9 and the serologically undefined DLA‐B gene product DLA‐B ‘blank’. For DLA‐A9, two subtypes (A9.1 and A9.2) were identified in Beagle and associations of A9.1 with the haplotype A9.B4 and of A9.2 with A9, B6 were seen. In contrast, the haplotype A9, B6 in Labradors was associated with A9.1. Whereas all six serologically defined DLA‐A antigens possessed distinct isoelectric points (IEP), not all DLA‐B antigens could be differentiated by 1D‐IEF: B5 as well as B13 coband with one B ‘blank’ specificity (BE1).Furthermore, a second B ‘blank’(BE2) could be identified by 1D‐IEF, underlining the advantage of this technique in the immu

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1995.tb00231.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SUMMARYHLA polymorphisms of class I and class II MHC were investigated in 40 Kuwaiti vitiligo patients and in 40 controls using microcytotoxicity assay. HLA‐B21, Cw6 and DR53 were increased significantly in patients compared to controls (P= 0.00001, 0.00001 andP= 0.0053 respectively) while HLA‐A19, DR52, were significantly decreased (P= 0.00236,0.05, respectively). Total T‐cells, T4 and T8 were measured as CD2, CD4 and CD8 respectively by flow cytometry. Vitiligo patients showed significant increase in CD4 compared to controls (P= 0.03). Our findings suggest that HLA‐B21 and Cw6 and DR53, are susceptible genes of vitiligo, while A19 and DR52 are protective genes in the Kuwaiti pop

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1995.tb00232.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SUMMARYThe heterogeneity of HLA‐B44 is confirmed and the sequence difference between the two major subtypes, B*4402,*4403, is attributed to one polymorphic site in the third exon. A method is described to discriminate B*4402 and B*4403, and the occurrence and linkage disequilibrium of B*44 subtypes is discussed. No example of B*4401 polymorphism in exon 2 was observe

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1995.tb00233.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SUMMARYWe propose to use the extreme diversity of HLA in order to detect, by anonymous check, double registration on computer files, of patients waiting for organ transplantation. A simulation on a panel of 69461 unrelated individuals, caucasoid, confirms the relevance of the method.

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1995.tb00234.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY