摘要:

SummaryChicken antibodies showing rigorous specificity for mouse immunoglobulins (Ig) allow the direct visualization of an Ig‐like surface component on thymusderived lymphocytes (T‐cells). These antibodies, therefore, provide a direct means of testing the hypothesis that the Ig‐like molecules function in antigen recognition on these cells. In an attempt to determine if avian antisera to mouse Fab determinants recognize antigen‐binding receptors, the surface distribution of three large fluorescent (red) protein antigens, keyhole limpet haemocyanin (KLH), horse spleen ferritin (HSF), and whale skeletal myoglobin (MYO) on BALB/c thymus antigen‐binding lymphocytes (ABL), was compared with that of fluorescent (green) labelled chicken antibodies to mouse Fab fragments [CAM(Fab')2]. The distribution of antigen and antisera against the Thy‐1 antigen and H‐2ddeterminants was also compared.The frequency of ABL ranged from 0.2 to 1% and binding to these cells could be inhibited by the appropriate unlabelled antigen and CAM(Fab')2. Thymus ABL were 100% positive for Thy‐1 antigen when doubly stained using fluorescent rabbit anti‐mouse immunoglobulin (RAM Ig) in an indirect fluorescence assay, but only 0‐10% of ABL were positive for binding of RAM Ig alone, indicating that nearly all ABL are T‐cells.Coincidence studies of patches of antigen and the various ligands were carried out by comparing coded fluorescence photomicrographs of randomly selected thymus ABL. Of thirty‐six ABL stained with anti‐thy‐1 antiserum, all but four cells had at least one patch of antigen which was not shared with antiserum. Of thirty‐six ABL stained with anti‐H‐2dserum, none had completely shared patches of antigen and antiserum. Of the forty ABL stained with CAM(Fab')2, every cell had every patch of antigen also patched with the anti‐Ig reagent. We intepret our results to indicate that the primary receptor for antigen on antigen‐binding T‐cells is either the Ig‐like molecule on the T‐cell surface or

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00691.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryA survey for constant region γ‐chain allotypes (de locus) was undertaken in different lagomorph genera. As yet onlyPronolagus rupestris, a paleolaginae, showed the presence of a determinant similar to rabbit d12 although it lacked the widespread e15 determinant.All seven individuals possessed the d12 like determinant which was studied by immunodiffusion, haemagglutination inhibition, radiobinding and binding inhibition assays. In addition, a new enzymic method for typing for d12, based on the presence of the asymmetric rabbit hing carbohydrate linked to the d12 characteristic threonine, is presented. This method suggests, however, that, unlike the d12 rabbit,Pronolagusdoes not seem to have a majority of IgG molecules with a glycosylated hin

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00692.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryA Japanese blood donor (H. T.) and his brother (M. S.) are the first homozygousMkMkindividuals described; their red cells lack, as expected, known antigens of the MNSs blood group system and also have no demonstrable MN‐active and Ss‐active glycoproteins. BothMkMkindividuals have a naturally occurring atypical antibody in their serum. The antibody in the serum of H. T. is inhibited by MNSs‐active glycoprotein preparations from normal erythro

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00693.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryMkwas demonstrated in three generations of an English family. The propositus was detected as a result of an incompatibility in cross‐match. General serological, biochemical and biophysical aspects have been studie

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00694.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryWe synthesized on Tn erythrocytes with human sera, UDP‐Gal, and activators T‐specific haptenic structures in satisfactory yield. The specificity of this biosynthesis was ascertained by agglutination with human and animal anti‐T, by specific absorption of human anti‐T as well as by agglutination inhibition assays.With isolated human erythrocyte T antigen as substrate we synthesized N‐ and M‐specific structures with sera from individual human donors in presence of CMP‐sialic acid by incubation for 24 hr at 37°C. Serology on the recovered product was carried out with nineteen monospecific human and animal sera under strictly standardized and controlled conditions with the mandatory tube assay. All M‐ as well as N‐derived T antigens tested acquired N specificity with all transferase sera of all MN types. In contrast, M‐activation of M‐ and N‐derived T antigens occurred only if the transferase donor had the M gene. The nine M transferase sera used all gave M‐activation of MM‐ and NN‐derived T antigens. None of twelve transferase sera from NN donors M‐activated any T antigen. NN antigen was transformed to a M‐specific one by all transferase sera from MM donors but by none from NN donors.We have not yet established the biochemical‐genetic relation of M to N; N may be the immediate precursor of M or M may originate directly from T. The sialytransferase responsible for M activation may be a N transferase ‘modified’ by the M gene product or

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00695.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryThe properties of five human alloantisera which inhibit the stimulating capacity of cells in an MLC reactions are described. All of the sera showed patterns of inhibition in families which did not correlate with HLA haplotypes. In a large proportion of the families investigated the sera did not react with parental cells but inhibited the cells from one or more of the children. Absorptions were performed with one of these sera using such non‐reactive parental cells. Paternal cells absorbed out the inhibiting activity while maternal cells did not. This indicates that experession of the antigen with which the inhibiting antiserum reacts is insufficient for inhibition and other factors, either environmental or inherited from the mother, influence the capacity of the antiserum to cause inhibition. This antigen was most likely a non‐HLA anti

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00696.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryWe are using the terms ‘low’ and ‘high’ expression when the H‐Y antigen on thymocytes of mouse parental strains is, respectively, weakly and strongly immunogenic for F1hybrids in a host‐versus‐graft popliteal lymph node enlargement assay. Using this assay we attempted to locate the genetic factor(s) responsible for the control of H‐Y expression within chromosome 17 since our previous study indicated linkage with the H‐2 complex. We produced to this aim recombinants from (B10.T x C3H/Di)F1hybrids where crossing over took place between H‐2 and T complexes. In this way we were able to locate a gene denoted Hye (for H‐Y expression) whose ‘high’ and ‘low’ alleles originated, respectively, from the B10 and C3H/Di strains. In the recombinants, the expression did not follow the H‐2 haplotype, but obviously depended on the position of crossing over between H‐2 and T. This pointed to the Hye gene mappin

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00697.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryThe ability of mouse amniotic fluid (MAF), alpha‐foetoprotein (AFP) and MAF depleted of AFP (MAF ‐ AFP) to suppress primary one‐way MLR's was investigated. It was found that MAF, AFP and MAF ‐ AFP were all suppressive of MLR's specific for MHC, K, D or I + S determinants. Suppression was observed when either lymph node or spleen cells were used as the responder cells. Nylon wool column passage of these cells did not significantly affect the immunosuppressive action of these substances. In contrast, MLR's specific for non‐MHC/M‐locus determinants demonstrated either diminished suppression or augmentation of the response, compared with the MHC stimulated MLR's. Our results show a differential effect of whole MAF and its fractions on the proliferative responses induced by various allogeneic stimuli and suggest that suppression is not due to a non‐specific effect on proliferation regardless of the stimulus or cell

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00698.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryWe have initiated studies to determine whether the antibody and T‐lymphocyte proliferative responses to lysozyme and its antigenic sites is genetically controlled in mice. Mice of theH‐2f, H‐2kandH‐2pwere high responders, while haplotypesH‐2b, H‐2d, H‐2randH‐2swere low responders. Studies with recombinants indicated that the immune response is controlled by two H‐2I region loci, one being in theI‐Asubregion and the other may be

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00699.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryImmune response to poly‐(l‐tyrosine‐l‐glutamic acid)‐poly‐d,l‐alanine‐poly‐l‐lysine ((T,G)‐A–L), human serum albumin (HSA), and (l‐glutamic acid50,l‐tyrosine50)n (GT) was found to be linked to the B complex in an outbred line of Leghorns segregating for theB1, B2, andB19alleles. Birds of the blood group genotypesB1B1, B2B2, andB19B19were low, intermediate, and high responders, respectively to either (T,G)‐A–L or HSA. Response to GT, however, differed, with theB2B2genotype being the only responder. No real genotype differences in immune response to DNP‐conjugates and sheep red blo

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00700.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY