摘要:

SummaryThe genetics of salivary secretion of ABH blood group substance was investigated. Eight secretor phenotypes were determined by Sephadex G‐100 chromatography. Presence or absence of three different fractions were shown to be controlled by three different secretor gene loci. These loci are calledSec1,Sec2andSec3.Sec1controls expression of the traditional secretor status.Sec2andSec3are linked, but distinct, loci controlling the expression of low molecular weight ABH substance in human saliva. A dominant mode of inheritance for theSec2andSec3systems is describe

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00332.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryMCG4 is a BALB/c sarcoma induced as a solid tumour with 0.2 mgs of methylcholantrene. The primary tumour was serially transplanted subcutaneously in syngeneic mice. The ascites form obtained was used to study the expression of H‐2 antigeneic specificities in a postlabelling radioassay.MCG4 did not express H‐2D.4 (private specificity of H‐2dhaplotypes) as well as H‐2.3, H‐2.8 and H‐2.13 (public specificities). In addition it expressed H‐2.5 (a public specificity not present in H‐2dcells). These results were confirmed by quantitative absorption analysis using MCG4 and positive‐negative normal lymphoid cells for a particular specificity.Results are discussed with regard to the control of expression of H‐2 antigens

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00333.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryThe effect of H2O2concentration on lactoperoxidase catalyzed cell surface radio‐iodination and subsequent isolation of Murine splenic lymphocyte Ia, H‐2K and Lyb‐3 surface antigens and membrane immunoglobulins was studied. For most membrane polypeptides analyzed 0.3 mM H2O2proved to be optimal for the recovery of radiolabelled antigens from detergent lysates of labelled cells by immunoprecipitation. Marked variations among surface antigens and membrane immunoglobulin polypeptide chains were observed for the iodination and recovery of these proteins above and below the optimal peroxide concentration. The results suggest that cell surface radio‐iodination conditions should be standardized to the requirements of the particular membrane protein being studied. The differential iodination and recovery of discrete membrane components above and below optimal conditions may prove useful in the analysis of surface membrane protein structure and membrane asso

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00334.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryFifty‐eight Japanese wild mice,Mus musculus molossinus, collected from twenty‐eight localities were surveyed for twelve H‐2 antigens using the haemagglutination method. Significantly higher frequencies of H‐2.3 and H‐2.5 specifities and relatively lower frequencies of the other public specificities were observed. This was confirmed by examining four specificities, H‐2.3, 5, 13 and 23, in 370 mice. Quantitative absorption of the alloantisera by erythrocytes and spleen lymphocytes ofmolossinumouse revealed definite absorption of H‐2.3, 5 and 8 antigens, though their antigenic strength was apparently weaker than the controls, B10 congenic mice. Comparative assay of the strength of H‐2.5 antigen in either homozygous and heterzygous conditions distinctly eliminated the possibility of gene‐dose effect for the reduced strength of the public antigens inmolossinusmouse. To explain this, divergence time betweenmolossinusanddomesticuswas computed based on the allelic frequency data already reported on ten loci in both subspecies. It is roughly 1.5 × 106years, which could allow for the occurrence of considerable genetic changes in many public antigens, except those conserved

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00335.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryThe six original haplotypes described in the Ag‐B system were compared with their counterparts in the H‐1 system. Antisera to the Ag‐B haplotypes raised in inbred rats and antisera to H‐1 haplotypes raised in congenic lines were tested against various panels of cell from inbred and congenic lines by the dextran and Ficoll haemagglutination methods. The private and strong public specificities detected in both systems were the same, but there were some minor differences in the intermediate and weak reactivities detected. The cross‐reactivity of the antisera raised in inbred rats was broader than that of antisera raised in congenic lines. The identity of the antigenic products detected in the two systems by the dextran and Ficoll tests was further confirmed by a variety of absorption analyses and by F1 tests. This study completes the systematic serological comparison of the haplotypes of the major histocompatibility complex of the rat described originally in the Ag‐B and

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00336.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryTen inbred strains of mice were administrated with dinitrophenyl‐bovine serum albumin (DNP‐BSA) at a dose of 30 or 300 μg/day by continuous infusion technique. Anti‐DNP plaque‐forming cells (PFC) in their spleens were assayed 10 to 12 days after the beginning of the infusion. NZB, BALB/c, C3H/He and NC strains were high responders to both doses of DNP‐BSA. KK, SII and TES strains were low responders: the antibody response was low to the dose of 30 μg/day, and high to the dose of 300 μg/day. Breeding tests between high responder BALB/c and intermediate responder DDD mice indicated that the immune response was largely controlled by a gene linked to the H‐2 complex. Similar studies with high responder NZB and low responder TES mice suggested an involvement of a few genes: at least one of the controlling genes may be linked to t

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00337.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

SummaryImmunoglobulin allotype (Gm) data has been analysed against immunoglobulin D (IgD) concentrations in a population study in Newfoundland. There was no significant difference between the distribution of IgD concentrations in people homozygous for the alleles Glm(f) and G3m(b) when compared with people homozygous for the alleles G1m(a) and G3m(g). These findings, involving 573 homozygous individuals as opposed to ninety‐eight in an earlier study on a New York population, do not confirm the earlier findings. Thus a genetic influence on IgD concentration by Gm genes or genes closely linked to them is not universally demonstrable by typing for these four markers and by using the Mancini technique for measuring IgD concentratio

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00338.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1979.tb00339.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY