摘要:

SUMMARYSixteen cysticerci excised from 15 surgery patients were examined for the presence of HLA molecules on their surface, to confirm the role of these molecules in parasite damage and to investigate if HLA products are host specific or perhaps host‐like antigens synthesized by the parasite. MoAbs against monomorphic and polymorphic HLA were chosen according to the patients HLA phenotypes. MoAbs against host and non‐host antigens were selected and tested on cyst slides by indirect immunofluorescence assays. Host molecules were present in 43.7% of the cysts, but non‐host antigens were also apparent in 62.5%. These results suggest mimicry as a possible mechanism to explain the presence of MHC products on the surface of the parasite; inflammation may also induce the expression of HLA that could become associated with the parasite.In vitrocellular immune response to specific antigens was also performed and positive responses correlated with the presence of HLA molecules on the cyst's surface. Moreover, damaged parasites had host molecules as well. Parasites from responder patients had all kind of HLA molecules or at least, antigenic determinants while the cysts from non‐responders did not have molecules on their surface. These data support the role of HLA in cyst dest

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00491.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYMultiple sclerosis is a demyelinating disease of the central nervous system with genetic, viral and autoimmune characteristics. Myelin basic protein (MBP) is a suspected target autoantigen since it induces experimental autoimmune encepha‐lomyelitis, an animal model closely resembling multiple sclerosis. The disease is mediated by Class II restricted, MBP‐reactive T cells possessing the T helper/inducer phenotype. In the present study, we have isolated MBP‐reactive T cell clones from the peripheral blood of a chronic progressive multiple sclerosis patient. The clones displayed blastogenic memory responses when rechallenged with the autoantigen and irradiated autologous lymphocytes. MBP recognition by the autoantigen‐reactive T lymphocytes was restricted by major histocompatibility complex Class II antigens. Both CD4+8−‐ and CD4–8+MBP‐reactive T cell clone

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00492.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

To investigate HLA‐linked genes controlling the susceptibility and resistance to insulin dependent diabetes mellitus (IDDM), HLA‐DQ alleles of 45 Japanese patients with IDDM were analysed, using sequence specific oligonucleotide (SSO).DQA1*0301andDQBI*04were positively associated (R.R = 6.6, Pc<0.05 and R.R. = 4.7 Pc<0.01) andDQAI*0103andDQBI*0104were negatively associated (R.R. =0–2, Pc<0.01) with IDDM.DQAI*0103andDQB1*0104were in strong linkage disequilibrium to encode for DQw6 molecule. Therefore, in a Japanese population, the DQw6 molecule seems to control the resistance to IDDM. To determine whether or not the DQw6 molecule itself can protect against glycosuria and insulitis in NOD mice, these animals were mated with HLA‐DQw6 transgenic‐C57BL/6 mice (DQw6‐B6) and the F1 progeny expressing the DQw6 molecule were backcrossed with NOD mice. Eighty‐five female backcross progenies were classified into four groups, according to the MHC classII phenotype; I‐Anod/I‐AnodDQw6(‐), I‐Anod/I‐AnodDQw6(+), I‐Anod/I‐AbDQw6(‐) and I‐Anod/I‐AbDQw6(+). At the age of 16 weeks, 9.1% of the DQw6(‐) I‐Ab(‐) mice had a glycosuria whereas none of the DQw6(+) I‐Ab(‐) mice had a glycosuria. At the age of 30 weeks 13.6% of the DQw6(‐) I‐Ab(‐) mice had a glycosuria and 7.7% of the DQw6(+) I‐Ab(‐) mice had a glycosuria. Histological examinations of the pancreas were performed in the 30 week old mice or after the development of glycosuria. About 50% of I‐Ab(‐) mice developed insulitis, regardless of the expression of the DQw6 molecule. Thus, the DQw6 molecule seemed to delay the onset of glycosuria but did not protect against glycosuria and

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00493.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe localization of TNF genes on the short arm of chromosome 6 between HLA B and the complement genes focused attention to that genetic region which harbours many immunologically relevant genes and is also thought to hold susceptibility genes for a variety of autoimmune diseases that are linked to specific alleles of particular loci in theHLA Dregion. Since the recently established HLA‐DR‐DQ variation accounts only for part of the genetic susceptibility to insulin‐dependent diabetes mellitus (IDDM) we searched for genomic variation of the tumour necrosis factor (TNF) alpha. We have identified a TNF‐alpha restriction fragment length polymorphism (RFLP) with N coI and analysed diabetic patients including their families, controls and homozygous typing cell lines (HTC) defined by the 10th International Histocompatibility Workshop. Segregation analysis in families and HTC results show a strong linkage of the TNF‐alpha 5.5 kb allele with DR types in particular withAIB8DR3. This tight linkage of TNF‐alpha alleles with extended haplotypes and the significant increase of heterozygotes in patients could lead to some explanation of the DR3 association with a variety of autoimmune diseases partic

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00494.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe restriction enzymesMspIandBg/IIidentify two different two‐allele restriction fragment length polymorphisms (RFLP) in the human IL‐6 genes of healthy Danes. Co‐dominant segregation was demonstrated for both marker‐systems and the test for Hardy‐Weinberg equilibrium showed no significant deviation from expectations. There is a strong correlation between the two marker systems. The two IL‐6 RFLP's were studied in Danish patients with rheumatoid arthritis, pauciarticular juvenile rheumatoid arthritis, and systemic lupus erythematosus. The frequencies of theMspIandBg/IImarker phenotypes did not differ between healthy controls and the three disease groups. No extra or missing DNA fragments were observed in the disease groups when compared wi

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00495.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYPreviously, an association between multiple sclerosis (MS) and HLA‐DPw4 has been reported in Scandinavians. In the present study, the distribution of HLA‐DP antigens was studied in 34 Japanese MS patients, a11 of whom fulfilled the criteria for definite MS. HLA‐DP typings for DPwl through w6 and the local specificity, CDP‐HEI, were performed using the primed lymphocyte typing (PLT) technique. In addition, the patients were typed for a DR2 +, Dw2 +/Dw12–related, PLT defined specificity. The distribution of DPw1‐w5 in 121 healthy, unrelated Japanese controls were from Nishimuraet al.,1984; Nishimura, personal communication). Sera from all 34 patients and 38 controls (both from the HTLV‐1 non‐endemic, Kyoto region) were examined for the presence of HTLV‐1 reacting antibodies by a highly sensitive radioimmuno assay (RIA) using two sources of HTLV‐1 antigens, namely total crude protein preparations from disrupted HTLV‐1 virions and affinity purified p24 HTLV‐1 core proteins.The frequency of DPw4 was significantly increased to 35.3% in Japanese MS patients compared to 16.5% in controls (Relative Risk, RR = 2.8, p = 1.9 × 10−2). 41.6% of the MS patients gave clear typing responses with a PLT reagent which recognized a Dw2+ related specificity, which is higher than the frequency of Dw2 (6.8%) in Japanese.Fourteen of the 34 patient sera contrasting to none of the sera from 38 controls contained antibodies of IgG and/or IgM subclasses reacting with the HTLV‐1 derived antigens. This difference is highly significant (P<1 × 10−5). However, there was no association between DP or Dw2 phenotypes and antibody status.These data provide evidence that genes within (or closely linked to) the HLA‐DP region confer susceptibility to MS in two genetically different ethnic g

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00496.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYA highly significant association of an amino acid sequence, ‘70Q71R72R73A74A’ located on the DRβ‐1 chain, with rheumatoid arthritis (RA) was confirmed in Japanese patients using polymerase chain reaction. The ‘70QRRA74A’ is the most plausible candidate of susceptibility epitope in Japanese RA patients. The patients carrying the sequence showed slightly higher but not significant familial incidence. The positivity of the ‘QRRAA’ showed no effect on the positivity of the rheumatoid factor, rheumatoid nodules, and extra‐articular signs. Also no significant differences in other clinical parameters (ESR, CRP, Hb, A/G) and age at onset were observed regarding the positivity. The risk that the ‘QRRAA’ positive subjects would suffer from RA was estimated to be about 1.3% which meant 7.2 times easier to get RA than t

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00497.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1989.tb00498.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY