摘要:

Transplantation of pancreaticoduodenal allografts was done in 30 totally pancreatectomized dogs. Ten hosts served as a control group and the remaining 20 were treated with azathioprine for immuno-suppression. Excepting two hosts (one in each group) which died of anastomotic complications, the control hosts lived for 3–20 days (averaging 10 days) and the azathioprine-treated hosts for 7–63 days (averaging 22 days). In both groups, hyperglycemia occurred rarely and only after a preponderance of islets were destroyed by rejection; it was therefore considered a terminal event. Serum amylase increased sharply at the start of rejection in control hosts and decreased rapidly to the preoperative level once the initial phase of rejection had passed. In azathioprine-treated hosts, small retarded rises in serum amylase were observed. Histologically the rejection patterns in the allografts were either acute or chronic. A chronic rejection pattern was found only in such allografts as stayed for more than 3 weeks in azathioprine-treated hosts. In the transplanted pancreas, there was no difference between the resistance of acinar cells and that of islet cells against the host's immunological attack. Different parts of the allograft showed different susceptibilities to the host reaction. The parenchyma of the pancreas and of the duodenum, as organs, succumbed readily to the immunological attack. Some lymph nodes were relatively resistant. Nerves and blood vessels maintained their histologic integrity longer than other tissues of the allograft.

ISSN:0041-1337    

出版商:OVID    

年代:1968

数据来源: OVID

摘要:

Neonatally thymectomized (A X C57BL/1)F1hybrids grafted with thymus from parental strain A donors developed evidence of graft-versus-host reactions, including weight loss and poor survival. Syngeneic spleen cells did not reverse this wasting syndrome. Immunological restoration was, however, observed in the surviving animals. When the neonatally thymectomized (A X C57BL/1)F1mice were grafted with syngeneic hybrid thymus no signs of wasting were observed and the animals were reconstituted immunologically. The thymectomized untreated animals showed a low incidence of wasting syndrome, which was readily reversed with syngeneic spleen cells. Such animals were unable to reject an allogeneic tumor graft but could reject an allogeneic skin graft with almost normal vigor. The results obtained after grafting of parental strain thymus into thymectomized hybrids suggest that a population of cells derived from the thymus plays an important role in expression of the graft-versus-host reactions observed.

ISSN:0041-1337    

出版商:OVID    

年代:1968

数据来源: OVID

摘要:

Families can be genotyped with respect to their inheritance of the units of inheritance of the HL-A system (haplotypes). The conclusion that skin exchanged between siblings who are genotypically identical with respect to HL-A is accepted for prolonged periods has been confirmed. The antigenicity of a haplotype can be determined by skin transplantation between siblings. Some haplotypes are rapidly reacted against, others elicit little effect alone but may be effective in combination. A possible recombinant was recognized serologically. The hypothesis was strengthened by the rejection times of skin grafts. Guidelines are suggested for the confirmation of recombination by (a) skin grafting and (b) the production of antibodies against the recombinant haplotypes, and for starting a linkage map.

ISSN:0041-1337    

出版商:OVID    

年代:1968

数据来源: OVID

摘要:

The ferritin-labelled antibody technique has been applied to the study of the distribution of the H-2 antigen. Using a specific antiserum for the isoantigens determined by the H-2dallele (H-2banti H-2d), the H-2 isoantigens were shown to be evenly distributed on the cell surface of lymphocytes, polymorphonuclear leukocytes, eosinophils, platelets, and erythrocytes. Lymphocytes were shown to possess the largest concentration of antigen at the cell surface. In preliminary experiments, nonspecific attachment of the ferritin-antibody marker to the intracellular components of dead or formalin-treated cells prevented the demonstration of an intracellular distribution of the H-2 antigen. The potential application of the ferritin-labelled antibody technique to the study of single H-2 isoantigenic specificities is discussed.

ISSN:0041-1337    

出版商:OVID    

年代:1968

数据来源: OVID

摘要:

Small particles of 10–350 Å escape from the hamster cheek pouch to the local nodes, but not as easily as they do from s.c. sites. Whole cells are unable to escape from the pouch, but do concentrate in local nodes from a s.c. injection. The systemic administration of cortisone does not affect the removal of small particles from the cheek pouch.

ISSN:0041-1337    

出版商:OVID    

年代:1968

数据来源: OVID

摘要:

Mouse isohemagglutinins and leukoagglutinins have been detected and quantitated by a sensitive reproducible assay. The procedure measures intercellular dissociation and migration of centrifuged cells in capillary tubes by the force of gravity. The assay does not require high concentrations of developers such as dextran and polyvinylpyrrolidone, and appears to be equally sensitive to “complete” and “incomplete” antibody.

ISSN:0041-1337    

出版商:OVID    

年代:1968

数据来源: OVID

ISSN:0041-1337    

出版商:OVID    

年代:1968

数据来源: OVID

摘要:

Evidence is presented for the existence of lymphatic vessels in the oral, muscular portion of the cheek pouch of the golden hamster. This finding is discussed in view of its bearing on the concept of the pouch as an immunologically privileged site.

ISSN:0041-1337    

出版商:OVID    

年代:1968

数据来源: OVID

摘要:

The haemopoietic capacity and graft versus host activity of fractions obtained after separating mouse spleen cell suspensions by centrifugation on a discontinuous albumin gradient were determined and compared with unfractionated spleen cells. One of the fractions obtained showed a 10-fold increase in the concentration of colony forming units and more than a 10-fold decrease of graft versus host activity. Cells from this fraction were found to protect allogeneic irradiated recipients without causing acute or delayed secondary disease. The method seems promising for application to primate bone marrow prior to transplantation, as a means to reduce the severity of acute secondary disease.

ISSN:0041-1337    

出版商:OVID    

年代:1968

数据来源: OVID

摘要:

Cell membrane preparations from spleens of surviving patients were treated by a series of steps that would lead, if the starting material had been mouse tissue, to soluble and highly purified H-2 transplantation antigens. The purified fractions from human tissue proved to have the specificity of HL-A (Hu-1) leucocyte group antigens that are known to play a major role in tissue graft rejection. The steps through which the H-2 and HL-A antigens showed exactly the same behaviour were: extraction of membrane lipoprotein, solubilization procedures, gel filtration separations and ion-exchange chromatography. The results indicate that human HL-A and mouse H-2 antigens are molecules with closely similar over-all composition. Serological tests show that the isolated HL-A antigens carry the correct specificities reflecting the original donor typing and do not carry specificities the donor did not possess. The soluble HL-A antigens are stable for at least 2 years when kept freeze-dried at −20 C. It is concluded that human leucocyte group antigens of the HL-A system are likely to be genetic homologues of the mouse H-2 antigens and hence that the extensive data that has accumulated from studies of the mouse model system may be used to guide further studies in man.

ISSN:0041-1337    

出版商:OVID    

年代:1968

数据来源: OVID