摘要:

Two α-globulin fractions which had in vivo and in vitro immunosuppressive activity were prepared from human serum. The larger fraction eluted from a molecular sieving gel in the albumin fraction and was heat labile whereas the smaller one was present in the eluate fraction containing materials of 8,000–25,000 daltons and was heat stable. It is believed that the smaller of the two fractions is the immunoregulatory α-globulin described by several other investigators. The comparative activities of various starting materials and of partially purified derivatives in several systems (in vivo and in vitro) were examined. The possibility that these substances are lymphocytic chalones is considered.

ISSN:0041-1337    

出版商:OVID    

年代:1976

数据来源: OVID

摘要:

An experimental model has been developed in the dog in which a renal allograft was placed in the neck, leaving one of the dog's own kidney in situ. Five nonimmunosuppressed pairs of dogs have been studied by using the leucocyte migration test (LMT) as an in vitro measure of cell-mediated immunity.Antigen preparations from leucocytes, kidney, liver, and skeletal muscle from both the kidney donor and the recipient were used in the LMT in order to study responses against transplantation and organ-specific antigens.Inhibition of migration with donor-specific leucocyte and kidney antigens was detectable prior to clinical evidence of rejection, which was confirmed histologically. Concurrently, inhibition was also observed with autologous kidney antigen and histological damage was noted in the recipient's own nontransplanted kidney, accompanied by increasing proteinuria.Autologous serum withdrawn daily and added to the test culture medium abolished the inhibition of migration, thus suggesting the development of blocking factor.

ISSN:0041-1337    

出版商:OVID    

年代:1976

数据来源: OVID

摘要:

Teratocarcinoma 402AX of strain 129/J mice lacks any detectable histocompatibility antigens on its surface when examined by immunofluorescence or cytotoxicity assays utilizing highly specific anti-H-2 histocompatibility alloantisera.

ISSN:0041-1337    

出版商:OVID    

年代:1976

数据来源: OVID

摘要:

As a first step in the study of the possible relationship between the T/t and H-2 complexes, the H-2 antigenic composition of the strains carrying factors t12, tw32, tw2, tw3, t1, t0, t6, tw1tw71tw73tw12, tw5, tw75, and t38was studied by using a battery of antisera containing antibodies against inbred-derived H-2 antigens.In addition, five t strains (t12, t6tw5, tw1and tw2) were selected for the production of antisera against the H-2 complexes carried by t chromosomes. Spleen, lymph node, and thymus cells from H-2b/t heterozygotes and tw2/tw2homozygotes were injected into appropriate F1hybrids between two inbred strains that carried the inbred-derived H-2 antigens of the donor. Four new H-2 antigens and one Ia antigen were uncovered and were assigned the symbols H-2.106 through H-2.109, and Ia.101, respectively. Three new H-2 haplotypes were also described, based upon the H-2 antigenic pattern of three t factors, t12, tw1, and tw5. These new haplotypes were given the symbols H-2t12, H-2tw1and H-2tw5. When the t factors were grouped according to their H-2 haplotypes, their distribution, with certain exceptions, corresponded to the complementation groups. Thus, t chromosomes in the same complementation group carried similar, if not identical, H-2 haplotypes, despite the fact that these chromosomes were derived from widely separated geographic areas. Such an association between the t and H-2 complexes is most unusual in light of what is known of the polymorphism of H-2 haplotypes in wild mice populations. It suggests more than a casual relationship, at least at the population level, between the t and H-2 loci.

ISSN:0041-1337    

出版商:OVID    

年代:1976

数据来源: OVID

摘要:

Tumour-specific antigen has been purified from the serum of animals bearing large progressively growing transplants of an aminoazo dye-induced hepatoma (D23). Antigenic activity was detected by the capacity of serum fractions to neutralize reactivity of antibody in hepatoma immune serum for cell surface expressed antigen on viable hepatoma cells as assessed by using the indirect membrane immunofluorescence test. Serum from animals bearing large i.p. grafts of tumour was fractionated by gel filtration on Sephadex G-150 columns at pH 7.3 and pH 2.8 to yield a crude antigen fraction which was further purified by affinity chromatography by using syngeneic rat hepatoma D23 immune IgG insolubilized on Sepharose 4B. The purified antigen exhibited a pI of approximately 4.6 on analytical gel isoelectric focusing. Molecular weight determination on Sephadex G-200 at pH 7.3 in nondenaturing buffer indicated a primary molecular weight of 55,000 and a small amount of aggregated material (mol. wt. greater than 250,000).

ISSN:0041-1337    

出版商:OVID    

年代:1976

数据来源: OVID

摘要:

The effect of the passive administration of antiserum obtained from recipients immunized with antigens from H-1 + non-H-1 incompatible donors on muscle allograft survival in rats was studied. In transplantation across the non-H-1 antigenic barrier (H-1 compatible but non-H-1 incompatible) a satisfactory degree of immunological enhancement was achieved. Recovery of contractile properties of muscle allografts after different immunological treatments of the recipients was compared.

ISSN:0041-1337    

出版商:OVID    

年代:1976

数据来源: OVID

摘要:

Lymphocytotoxic alloantisera were obtained by cross immunization among outbred guinea pigs from a closed colony. Groups of antisera recognizing the same specificities were established by panel analysis, cross immunization among appropriate phenotypes, and absorption studies. Three antigens designated B1, B2, and B3 (previously B, C, and D) were detected in our outbred colony and also found to be present in a wide variety of guinea pig strains. These antigens were shown by population and breeding studies to be allelic gene products of a first guinea pig locus (GPLA) (designated locus B). Four new guinea pig families homozygous for their B locus antigens are now in the seventh generation of inbreeding.NIH guinea pig strains 2 and 13, which share the Bl antigen, differ by a number of other antigens which have been designated I1, I2, I3 (previously A), and I4. These antigens differ from the B locus gene products in their molecular size and tissue distribution and it is, therefore, postulated that they represent an equivalent of the la antigens in mice (designated I region).Cross immunizations among inbred or outbred guinea pigs identical for currently known B locus and I region antigens yield further antisera, possibly recognizing additional Ia specificities and/or a second GPLA locus designated locus S. Whereas the conventional eosin lymphocytoxicity technique easily permits the detection of the widely distributed antigens (such as the B locus antigens), a more sensitive51Cr release technique is required to demonstrate the “Ia equivalent” antigens of the guinea pig, possibly due to their restricted presence on the membrane of only some lymphoid cell populations.

ISSN:0041-1337    

出版商:OVID    

年代:1976

数据来源: OVID

摘要:

The population of peritoneal macrophages from mice immunised with allogeneic tumor cells contains two types of cytotoxic cell. One lyses the specific target cell the other initiates activation of macrophages and hence leads to inhibition of growth of the specific target cell. The yield of lytic effector macrophages was found to depend on the route and the nature of the cell used for immunisation and the condition of the mice. The yield correlated with the yield of complement-dependent cytotoxic antibodies. In contrast, production of specific “activator” macrophages did not depend critically on these factors. The results underline the difference between the two types of cell and suggest that they are produced independently of one another.

ISSN:0041-1337    

出版商:OVID    

年代:1976

数据来源: OVID

摘要:

Murine spleen cells were separated on the basis of adherence to glass beads into distinct subpopulations that differ in their ability to produce acute graft-versus-host disease (GVHD). Nonadherent CBA spleen cells produce acute GVHD in 6–10 days in lethally irradiated (C57BL/6 × CBA) F1mice as do unfractionated spleen cells. Spleen cells which are adherent to glass beads, however, enable 71% of the mice to survive without symptomatology of acute GVHD. The low proliferative response of these cells to phytohemagglutinin (PHA) correlated with the mitigated GVHD seen in animals grafted with this fraction. Proliferative cells as determined by the spleen colony assay and the in vitro agar colony-forming assay are present in this fraction as are cells responsive to mitogenic stimulation with lipopolysaccharide (LPS). B6CBF1mice grafted with CBA adherent cells exhibit a gradual return over a period of 5 months to normal PHA and LPS stimulation levels as shown by splenic cell responses of these mice to mitogens. Surviving mice grafted with adherent cells were chimeric as determined by electrophoretic hemoglobin pattern analysis and serial bone marrow transplantation.

ISSN:0041-1337    

出版商:OVID    

年代:1976

数据来源: OVID

摘要:

Antirat hemagglutinins may be found in the sera of both normal and sick persons. High titers of these antibodies are influenced by the major blood groups, the antigen B tending to suppress these antibodies, and by age where juveniles have higher titers than adults. Elevated titers may be stimulated by immunization, injection of pooled γ-globulin, infection, and transplantation.

ISSN:0041-1337    

出版商:OVID    

年代:1976

数据来源: OVID