ISSN:0041-1337    

出版商:OVID    

年代:1978

数据来源: OVID

摘要:

SUMMARYBlocking activity of mouse pregnancy serum was investigated using a well defined one-way mixed lymphocyte culture and a cell-mediated microcytotoxicity test. Allogeneic (C57BL (H-2b) × CBA/Ca (H-2k)) and syngeneic (C57BL (H-2b) × C57BL (H-2b)) pregnancy and postpartum sera were found to stimulate rather than inhibit the mixed lymphocyte response. In contrast, the same sera were able to abrogate target cell killing by hyperimmune lymphocytes in a cell-mediated microcytotoxicity test. Although inhibitory activity was present in both allogeneic and syngeneic 18-day pregnancy sera, indicating a nonspecific effect, only allogeneic postpartum sera had significant blocking activity when compared with either the equivalent syngeneic group or the normal serum control. The specificity implied by this gained support from preliminary third-party experiments. Blocking activity was found in primiparous as well as multiparous sera.These results provide further evidence for the existence of maternal serum-blocking factors which may operate to protect the conceptus against maternal cell-mediated immunity during pregnancy, and indicate that they may act at the efferent rather than the afferent phase of the immune response.

ISSN:0041-1337    

出版商:OVID    

年代:1978

数据来源: OVID

摘要:

SUMMARYIn a series of 30 bone marrow grafts (in 29 recipients) from HLA-identical siblings for aplastic anemia, no correlation was demonstrated between rejection and pregraft HLA antibodies (P> 0.50). However, after grafting, HLA antibodies persisted in nine cases and graft rejection occurred, whereas in all but one of the remaining eight cases the HLA antibodies disappeared and a permanent engraftment was observed (P= 0.0008). These results suggest a relationship between engraftment and persistence or disappearance of HLA antibodies as an indication of the level of immunosuppression obtained with the conditioning regimen.

ISSN:0041-1337    

出版商:OVID    

年代:1978

数据来源: OVID

摘要:

SUMMARYA comparison is made between the early results of pancreatic and pancreaticoduodenál transplantation in the pig. In all cases pancreatic venous drainage was physiological, i.e., into the portal vein. Endocrine graft function was tested by oral or i.v. glucose tolerance on the 6th and 7th postoperative days. In addition, daily samples were taken for insulin, immunoreactive glucagon (IRG), and amylase estimation and animals were killed on day 14. Glucose tolerance results of animals with transplants were compared with sham-operated, pancreatec-tomised, or duodenectomised pigs.Although daily insulin and glucagon values in both groups of animals with transplants were within normal limits for the first postoperative days, blood sugar levels were persistently elevated except for the 2 days when animals were starved prior to glucose tolerance tests. In addition, in both oral and i.v. glucose tolerance, the insulin and glucagon responses were depressed as compared with sham-operated animals. The responses to oral or i.v. glucose in duodenectomised pigs showed no resemblance to those of pigs with pancreatic or pancreaticoduodenal transplants, suggesting that any peculiarities of response in the pigs with transplants were not the effect of the duodenum. Graft rejection occurred but pancreatitis or evidence of duodenal leakage was not seen at autopsy. These studies suggest that in the pig, transplantation of either the pancreas alone or the pancreas with the duodenum has similar early results and that there might be a place for a clinical trial of transplantation of the pancreas alone.

ISSN:0041-1337    

出版商:OVID    

年代:1978

数据来源: OVID

摘要:

SUMMARYAntihuman lymphocyte globulin (ALG) was either coupled to the lymphocytoxic drug chlorambucil or covalently bound to the cytotoxic alkalating agent melphalan via a polyglutamic acid carrier. Both types of complexes strongly inhibited the proliferative response in human mixed lymphocyte cultures and the ability of mixed lymphocyte culture-activated T effector cells to lyse51Cr-labelled lymphoblast target cells, and were more potent than ALG or drug alone. These experiments indicate that it is possible to bind cytotoxic agents to ALG without destroying either the properties of the drug or the ability of the antibody to bind to lymphoid cells.

ISSN:0041-1337    

出版商:OVID    

年代:1978

数据来源: OVID

摘要:

SUMMARYH-l1(Ag-B1-histocompatible rats were cross-immunized to study the effects of presensitization on the mixed lymphocyte culture (MLC) reactivity in normally nonresponsive MLC interactions. Lewis (LEW) rats, H-l1(Ag-B1), were given s.c. injections of Fischer (F), H-l1(Ag-B1), lymphocytes at weekly intervals for 3 weeks. Immunized LEW (Lew-iF) and F lymphocytes showed increased proliferative responses in MLC after the third immunization. The augmented proliferative responses in the MLC between these H-l1(Ag-B1)-histocompatible rat strains following immunization may reflect minor histocompat-ibility antigenic differences. On the other hand, decreased proliferative responses in MLC between Lew-iF and Ag-B (H-1)-disparate rat strains were demonstrated. Alloimmunization across minor histocompatibility differences led to different types of regulatory effects on the MLC interactions. With low MLC responses, e.g., minor histocompatibility differences, the regulation tends to be positive; whereas for strong MLC responses, e.g., major H-l (Ag-B) differences, the regulation tends to be negative or suppression.

ISSN:0041-1337    

出版商:OVID    

年代:1978

数据来源: OVID

摘要:

SUMMARYMitomycin C-treated ascites cells of Ia antigen-positive GRSL14 tumor cells and spleen cells from GRSL14 tumor-bearing mice stimulated lymphocyte proliferative responses in normal syngeneic GR spleen cells. Furthermore, mitomycin C-treated T cells purified from spleen cells of tumor-bearing mice also stimulated normal GR spleen cells. Anti-la sera inhibited the stimulating ability of tumor cells and of spleen cells of tumor-bearing mice. These data suggest a role forIregion gene products in immune surveillance for syngeneic tumors.

ISSN:0041-1337    

出版商:OVID    

年代:1978

数据来源: OVID

摘要:

SUMMARYCultured lines of three newly established methylcholan-threne-induced tumors, MBK and MBL in CBA and MC57M in C57BL mice, and two mammary tumors, SBfnHC in CBA and MC57M in ASW mice, were superinfected in vitro with Moloney sarcoma or leukemia virus (MSV, MLV). After superinfection, they expressed the Moloney virus-determined cell surface antigen (MCSA) and murine C-type viral p30 antigen, and produced NB-tropic C-type virus. The virus-infected tumors became more rejectable in normally susceptible syngeneic mice compared with the original noninfected line. There wa no difference in 400-rad irradiated hosts. Mice that have rejected the virus-infected tumors showed an increased resistance to the corresponding noninfected tumor. The protective effect was comparable, with only one exception, to the immunizing effect of irradiated, noninfected cells.In vitro tests showed that small numbers of viable MSV-infected MBL generated cytotoxic spleen cells against both uninfected and MSV-infected MBL in syngeneic mice, while the same numbers of viable noninfected MBL did not induce cytotoxic cells. Relatively large numbers of irradiated MBL and MSV-MBL had a similar activity in inducing cytotoxic spleen cells against MBL in syngeneic hosts.

ISSN:0041-1337    

出版商:OVID    

年代:1978

数据来源: OVID

摘要:

SUMMARYThe BALB/c (H-2dMls2)-DBA/2 (H-2dMls1) mouse model was used to examine whether Mls loci stimulated reactions in mixed lymphocyte culture (MLC) could be used to monitor cellular interactions governed by other non-H-2-encoded antigens in graft rejection. Several new findings have emerged: (1) Cells primed in MLC and then irradiated (x) were found to strongly stimulate fresh syngeneic splenocytes, as well as potentiate and accelerate the response of fresh allogeneic splenocytes in MLC. (2) Potentiation was also produced by primed cells added as third components to MLCs made up of fresh cells. This was magnified if the stimulating cell population was the same as that to which the primed cells were directed. (3) These primed cells used as stimulating cells in syngeneic or allogeneic cultures with fresh responding cells could be used to generate entirely in vitro a second population of blasts that exhibited suppressor cell activity. A two-step process was required to evoke these suppressor cells: (1) BALB/c (B) cells were stimulated with DBA/2X (Dx) to provide a primed anti-DBA (B'D) blast population; and (2) when B'Dx cells were then used as stimulating cells in MLC with fresh syngeneic BALB/c or allogeneic DBA/2 as responders, a suppressor cell population B'B‘D or D'B’D was generated in the putative absence of cytotoxicity. Preliminary results indicate that survival of DBA/2 skin allografts can be prolonged by immunizing BALB/c recipients with B'Dx in complete Freund's adjuvant. The implications of these findings are discussed.

ISSN:0041-1337    

出版商:OVID    

年代:1978

数据来源: OVID

摘要:

SUMMARYAn isolated perfused rat kidney has been used to determine the response to prolonged warm ischaemia and to examine renal preservation procedures. All measurements of function declined to near zero after 90 min of warm ischaemia. Measurement of total sodium reabsorption (TNa) was the most sensitive indicator of renal damage. Inosine was without effect on subsequent renal function after 60 or 90 min of warm ischaemia, or after 24 hr of cold ischaemia. Surface cooling or hypertonic citrate solution, in the cold or at 37 C, significantly improved renal function, and their use in clinical renal preservation rather than that of inosine was supported by the present results.

ISSN:0041-1337    

出版商:OVID    

年代:1978

数据来源: OVID