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1. |
The rationale for hand transplantation. |
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Transplantation,
Volume 74,
Issue 6,
2002,
Page 749-753
V. Lees,
S. McCabe,
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ISSN:0041-1337
出版商:OVID
年代:2002
数据来源: OVID
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2. |
USE OF CYCLOSPORINE |
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Transplantation,
Volume 74,
Issue 6,
2002,
Page 754-755
T. Asai,
T. Nakatani,
S. Yamanaka,
S. Tamada,
T. Kishimoto,
K. Tashiro,
T. Nakao,
M. Okamura,
H. Iwao,
K. Miura,
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ISSN:0041-1337
出版商:OVID
年代:2002
数据来源: OVID
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3. |
Legionnaires’ Disease in a Transplant Recipient Acquired from the Patient’s Home: Implications for Management |
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Transplantation,
Volume 74,
Issue 6,
2002,
Page 755-756
A. Sax,
S. Dharan,
D. Pittet,
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ISSN:0041-1337
出版商:OVID
年代:2002
数据来源: OVID
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4. |
CARDIAC HYPERTROPHY POSTLIVER TRANSPLANTATION: A ROLE FOR FK506 AND CYCLOSPORINE A? |
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Transplantation,
Volume 74,
Issue 6,
2002,
Page 757-758
C. Roberts,
D. Stern,
S. Radio,
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ISSN:0041-1337
出版商:OVID
年代:2002
数据来源: OVID
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5. |
Protective effect of T cell depletion in murine renal ischemia-reperfusion injury. |
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Transplantation,
Volume 74,
Issue 6,
2002,
Page 759-763
Naoko Yokota,
Frank Daniels,
John Crosson,
Hamid Rabb,
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摘要:
Background.Ischemia-reperfusion injury (IRI) is the main cause of acute renal failure in both allograft and native kidney. Studies using T cell knockout mice have established an important role for T cells in renal IRI. T cell depletion strategies are effective in human allograft rejection. However, it is not known whether those are effective in renal IRI. Therefore, the effect of T cell depletion in a murine model of renal IRI using well-characterized antibodies (Abs) that have been effective in preventing experimental allograft rejection was studied.Methods.T cell depleting Abs to CD4 (GK1.5), CD8 (2.43) or pan-T cells (30.H12) were purified from hybridoma culture supernatant. Thymectomized C57BL/6 mice, treated with different combinations of T cell depleting Abs, underwent 30 min of bilateral renal IRI, followed by assessment of renal function, structure, and degree of T cell depletion in spleen, lymph nodes, and peripheral blood by flow cytometry.Results.Mice given both GK1.5 and 2.43 had considerable CD4 and CD8 cell depletion but no protection of renal function after ischemia-reperfusion (I/R) as measured by the rise in serum creatinine. However, when GK1.5 and 2.43 was administered combined with 30.H12, which more effectively depleted CD4 T cell numbers, a significant protection of renal function and structure was observed after I/R. Antibody combinations did not significantly alter other leukocyte populations.Conclusions.These data demonstrate that T cell depletion can improve the course of experimental renal IRI. However, more aggressive T cell depletion strategies were required compared with that needed to prevent experimental allograft rejection.
ISSN:0041-1337
出版商:OVID
年代:2002
数据来源: OVID
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6. |
Comparison of coronary endothelial dysfunction in the working and nonworking graft in porcine heterotopic heart transplantation. |
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Transplantation,
Volume 74,
Issue 6,
2002,
Page 764-772
Louis Perrault,
Jean-Pierre Bidouard,
Nathalie Desjardins,
Nicole Villeneuve,
Jean-Paul Vilaine,
Paul Vanhoutte,
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摘要:
Background.The nonworking heterotopic heart transplantation model has been used extensively for the study of rejection and coronary endothelial function in different species. The effect of left ventricular loading in a working heart transplantation model, which may be associated with different coronary flow patterns and local nitric oxide release, on the development of coronary endothelial dysfunction and intimal hyperplasia, is unknown.Methods.Porcine retroperitoneal “nonworking” heterotopic transplantations (n=10) and “working” heart (with left ventricular filling) transplantations (n=7) were performed. The left ventricular pressure was 0±0 mm Hg and 91±11 mm Hg in the nonworking and working groups, respectively. In the latter, the left ventricle to systemic arterial pressure ratio was 0.76±0.08.Results.Sixty days after transplantation, epicardial coronary arteries from working and nonworking allografts developed a comparable selective endothelial dysfunction of Gi-protein mediated relaxations. There were no statistically significant differences in the prevalence of intimal hyperplasia, but the severity of intimal hyperplasia was significantly greater in allograft coronary arteries from the working hearts.Conclusion.Working heterotopic allografts develop an endothelial dysfunction comparable with that of nonworking allografts, which validates the use of the simpler nonworking graft for the study of endothelial function. The similar prevalence of intimal hyperplasia with the development of more severe coronary lesions in working hearts may be due to differences in local nitric oxide release in these two models.
ISSN:0041-1337
出版商:OVID
年代:2002
数据来源: OVID
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7. |
Interleukin-10 modulation of alloreactivity and graft-versus-host reactions. |
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Transplantation,
Volume 74,
Issue 6,
2002,
Page 772-778
Xiao-nong Wang,
Claudia Lange,
Ute Schulz,
Lisbet Sviland,
Günther Eissner,
Kristyna Oliver,
Graham Jackson,
Ernst Holler,
Anne Dickinson,
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摘要:
Background.The biological properties of interleukin (IL)-10 in tolerance induction and inhibition of alloreactivity have suggested a therapeutic use of this cytokine as an additional or alternative prophylaxis for graft-versus-host disease (GvHD). However, the effects of exogenous IL-10 on GvHD are mainly studied in animal models, and the results remain conflicting. This study aims to demonstrate, for the first time, whether the addition of exogenous IL-10 can reduce the severity of graft-versus-host reactions (GvHR) in humans.Methods.The regulatory role of exogenous IL-10 in GvHR was investigated using an in vitro human skin explant model. The effects of IL-10 on skin GvHR were tested in parallel with allo-antigen induced T-cell proliferation, cytolytic reactivity, and cytokine production.Results.In the presence of IL-10, the mixed lymphocyte reaction (MLR) primed responder cells showed significantly lower proliferative and cytolytic responses compared with the responder cells from the control MLR carried out in the absence of IL-10. The responder cells from IL-10 containing MLR induced significantly less severe skin GvHR and displayed a significantly reduced T-cell activation and cytokine production. A significant correlation was observed between the levels of TNF-&agr; production and the sensitivity to IL-10 modulation of GvHR.Conclusions.The addition of exogenous IL-10 strongly inhibited the broad alloreactivity initiated by primary MLR and significantly reduced the overall severity of skin GvHR induced by MLR primed responder cells. Responder cells producing high TNF-&agr; following allogeneic stimulation appeared to be less sensitive to IL-10 modulation of GvHR.
ISSN:0041-1337
出版商:OVID
年代:2002
数据来源: OVID
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8. |
Storage solution containing fructose-1,6-bisphosphate inhibits the excess activation of kupffer cells in cold liver preservation1. |
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Transplantation,
Volume 74,
Issue 6,
2002,
Page 779-783
Fumitoshi Hirokawa,
Takehiro Nakai,
Hiroki Yamaue,
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摘要:
Background.In liver transplantation, the activation of Kupffer cells at the time of cold preservation and reperfusion is considered to play an important role. In the present study, the usefulness of cold storage solution containing fructose-1,6-bisphosphate (FBP) was compared with University of Wisconsin (UW) solution in the function of Kupffer cells.Methods.Kupffer cells were separated from rat liver stored at 4°C in each storage solution. Four kinds of storage solutions were used: UW, simplified UW without FBP (0-FBP), and solutions with 10 or 20 mM FBP (10-FBP, 20-FBP). Lipopolysaccharide (LPS) labeled by fluorescein was loaded after 12 or 24 hr of cold preservation in each solution. The rates of cells uptaking LPS as phagocytic ability were measured using flow cytometry. Tumor necrosis factor-&agr;, cytokine-induced neutrophil chemoattractant, and nitric oxide (NO) were measured in the supernatant.Results.Tumor necrosis factor-&agr; values in the 20-FBP group were significantly lower than those in the UW group. Cytokine-induced neutrophil chemoattractant values at 60 min after loading LPS were significantly lower in the 20-FBP group than in the UW group. NO values at 24 hr after loading LPS were significantly lower in the 20-FBP group compared with the UW group. The 20-FBP group was highest in the rates of cells uptaking LPS after 24-hr cold preservation.Conclusions.The storage solution containing FBP controlled the secretion of cytokines and NO from Kupffer cells and maintained phagocytic ability. This solution was considered to be more useful than UW solution for Kupffer cell protection.
ISSN:0041-1337
出版商:OVID
年代:2002
数据来源: OVID
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9. |
Magnesium supplementation prevents experimental chronic cyclosporine a nephrotoxicity via renin-angiotensin system independent mechanism. |
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Transplantation,
Volume 74,
Issue 6,
2002,
Page 784-791
Toshihiro Asai,
Tatsuya Nakatani,
Shinya Yamanaka,
Satoshi Tamada,
Taketoshi Kishimoto,
Koichiro Tashiro,
Takafumi Nakao,
Mikio Okamura,
Shokei Kim,
Hiroshi Iwao,
Katsuyuki Miura,
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摘要:
Background.We have previously shown that correction of hypomagnesemia by magnesium (Mg) supplementation ameliorates chronic cyclosporine A (CsA) nephropathy via inhibiting gene expression of fibrogenic molecules. Experiments were conducted to further elucidate upstream mechanism of the beneficial effects upon CsA nephrotoxicity.Methods.CsA (15 mg/kg/day, subcutaneous [SC]) was administered daily to rats maintained on low sodium diet for 7, 14, and 28 days. Because blockade of renin-angiotensin system improves chronic CsA nephropathy, the effects of Mg supplementation and those of angiotensin-converting enzyme inhibitor (ACEI) were compared on renal function, renal histology, mononuclear cell infiltration, and gene expression profile.Results.CsA induced a decline in glomerular filtration and developed characteristic striped fibrosis that were mostly evident at day 28. Mg attenuated CsA-induced impaired renal function, whereas ACEI did not. Interstitial inflammation as evidenced by monocyte/macrophage infiltration preceded the renal fibrosis and increased progressively with the CsA treatment period. Concomitantly, CsA markedly up-regulated expression of chemoattractant proteins, osteopontin, and monocyte chemoattractant protein-1. These changes were abolished by Mg but were only partially affected with ACEI. CsA promoted renal mRNA expression of fibrogenic molecules and extracellular matrices that were almost completely abolished by Mg but partially suppressed by ACEI. Similarly, CsA-induced chronic fibrotic lesion was markedly attenuated by Mg supplementation but was partially attenuated by ACEI.Conclusion.Mg supplementation abolished CsA-induced precedent interstitial inflammation possibly via inhibition of chemoattractants expression and consequently attenuated tubulointerstitial fibrosis. In this protective mechanism, factors independent of the renin-angiotensin system appears to be mainly involved.
ISSN:0041-1337
出版商:OVID
年代:2002
数据来源: OVID
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10. |
DISTINCT ENZYME PROFILES IN PATIENTS WITH CRYPTOGENIC CIRRHOSIS REFLECT HETEROGENEOUS CAUSES WITH DIFFERENT OUTCOMES AFTER LIVER TRANSPLANTATION (OLT): A LONG-TERM DOCUMENTATION BEFORE AND AFTER OLT |
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Transplantation,
Volume 74,
Issue 6,
2002,
Page 792-798
Thomas Berg,
Ruth Neuhaus,
Reinhild Klein,
Korinna Leder,
Hartmut Lobeck,
Wolf-Otto Bechstein,
Andrea Müller,
Bertram Wiedenmann,
Uwe Hopf,
Peter Berg,
Peter Neuhaus,
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摘要:
Background.Sound information is lacking about the clinical presentation of cryptogenic cirrhosis and its outcome after orthotopic liver transplantation (OLT).Methods.Among 856 patients who have been transplanted at our center, 40 patients had no evidence of any known etiologies and were therefore defined as suffering from cryptogenic cirrhosis. Their median follow-up period before OLT was 78 months (range, 1–264), and after OLT 97 months (range, 1–132). Laboratory and histological data were evaluated according to features being compatible either with a toxic, hepatitic, or cholestatic condition.Results.The clinical and histological findings differed specifically between these three groups. The toxic-like group (GGT 4–18 × upper limit of normal [ULN]) expressed significantly higher IgA levels, had histologically more often fatty liver changes, and risk factors for non-alcoholic steatohepatitis predominated (56% compared with 3% in the other groups,P=0.01). The hepatitic-like group (ALT 2–18 × ULN) showed histologically features of chronic hepatitis or hepatitic cirrhosis, and only among these patients a median International Autoimmune Hepatitis (IAH) score of 13 was found suggesting autoimmune hepatitis (AiH). In the cholestatic group (AP 2–8 × ULN) histology was compatible with a non-toxic inflammatory process but IAH score excluded AiH in all. After OLT, actuarial graft and patients survival was 90% at 5 years. Mild or moderate graft hepatitis occurred in 9 patients (23%) and was significantly associated with a pre-OLT IAH score ≥ 10 (P=0.008).Conclusions.This study provides arguments that cryptogenic cirrhosis is a heterogeneous disease in which autoimmune mechanisms might be predominately involved and being responsible for recurrence of chronic liver disease observed in some instances after OLT.
ISSN:0041-1337
出版商:OVID
年代:2002
数据来源: OVID
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