摘要:

The vascular homograft response has been studied using fresh carotid arterial grafts in 57 dogs. The animals were divided into two groups, the first receiving no immunosuppressive therapy, and the second receiving azathioprine and prednisone. The histological changes have been followed in both groups of animals for 3 months. In the control group the phases of recognition, reaction, repair and organization can be observed. In the treated animals the phase of recognition is not delayed but the phases of reaction, repair and organization are delayed for a variable period of time up to 3 months.

ISSN:0041-1337    

出版商:OVID    

年代:1966

数据来源: OVID

摘要:

No reduction in the number of rats with pregnancies nor in the number of fetuses per pregnancy could be demonstrated when groups of Sprague-Dawley females were injected with placental tissue in various forms and then mated to Long-Evans males. The placental preparations were either: (1) homogenates of fetal placentas removed from Long-Evans rats; (2) whole cells of fetal placentas derived from the same Long-Evans male with which the injected female was mated; or (3) cells of fetal placenta (derived from the same Long-Evans male with which the injected female was mated) homogenized with Freund's adjuvant.

ISSN:0041-1337    

出版商:OVID    

年代:1966

数据来源: OVID

摘要:

51Cr-labelled (CBA X A)F1and A strain lymph node cells were injected by either the i.v. or the i.p. route into adult A strain mice, and the distribution of radioactivity in various organs was studied over a period of 48 hours. The extent to which radioactivity in an organ is a measure of the number of living donor cells is considered in the light of control experiments with frozen-thawed and intact heat-killed cells. From these and other experiments it is concluded that the elution of51Cr and its reattachment to host cells plays little part over this period, and that the differences between the results obtained by i.v. and i.p. inoculation are largely attributable to differences in the distribution of living cells. Both with allogeneic (hybrid) and syngeneic (A strain) lymph node cells, the activity recovered in the organs was many times greater when the cells had been administered by the i.v., as opposed to the i.p., route. This was true for the liver, lungs, spleen, and lymph nodes, but not for the thymus and salivary glands, from which little activity was recovered by either route. Using both routes the greatest part of the recovered radioactivity was found in the liver and spleen, but relative to organ weight uptake was highest in the lymph nodes and spleen. It is suggested that the different distribution of cells, together with the fact that cells injected i.p. are exposed to peritoneal as well as to liver macrophages, accounts for the very different results obtained when attempting to induce sensitization or tolerance by these two routes. Adult A strain mice presensitized with CBA antigens showed what appeared to be immune elimination of hybrid lymph node cells—an observation suggesting that it might be possible to use this approach as a test of homograft sensitivity.

ISSN:0041-1337    

出版商:OVID    

年代:1966

数据来源: OVID

摘要:

Using a test system of graft-versus-host reaction (GVHR) in F1hybrid mice (A/J X C57BL/6J) initiated by the inoculation of neonatal F1mice with parental strain spleen cells, the following conclusions are made in relation to the modification of the GVHR by 6-mercaptopurine (6-MP). 1. 6-MP in total dose of 120 mg/kg body weight or more given over a 6-day period results in splenic hypoplasia in neonatal mice. 2. 6-MP in total dose of 90 mg/kg body weight or less does not result in splenic hypoplasia. 3. The incidence of runt disease is 82% (0.99 confidence limits of 69–91%) in the above strain combination. 4. 6-MP treatment in total dose of 60 to 90 mg/kg body weight reduces this incidence to 55% (0.99 confidence limits of 43–67%). 5. The GVHR may be used as a test system in screening drugs for their effectiveness in altering the immunologic response to allogeneic homografts.

ISSN:0041-1337    

出版商:OVID    

年代:1966

数据来源: OVID

摘要:

Rabbits carrying skin allografts and autografts were killed at daily intervals up to 8 days after grafting, 2 hours after they were given an i.v. dose of tritiated thymidine. Counts of labelled epithelial cells in autoradiographs of grafts revealed a progressive increase in the rate of DNA synthesis in allografted skin, as the rejection process progressed, when compared to autografted skin in the same rabbit. It is possible that at concentrations below those that cause cell destruction the infiltrating lymphoid cells and/or transplantation antibody may produce epithelial cell proliferation.

ISSN:0041-1337    

出版商:OVID    

年代:1966

数据来源: OVID

摘要:

Labeled lymphoid cells obtained from the lymph nodes draining a skin graft were administered to syngeneic mice of the opposite sex. Labeling of proliferating cells in the lymph nodes was accomplished by the repeated injection of tritiated thymidine into the base of the graft. Labeled cells, presumably donor lymph node cells, were present in small number evenly distributed in autoradiographs of sections of the thymus of both neonatal and adult recipients. At the time of observation, 1, 2, or 3 days after donor cell inoculation, donor cells were not observed in mitosis. The exemption of the thymus from direct involvement in the immune response cannot be accounted for by the exclusion of lymph node cells and therefore it is more likely to depend on factors operating within the thymus itself.

ISSN:0041-1337    

出版商:OVID    

年代:1966

数据来源: OVID

摘要:

The application of repeated skin homografts from the same rabbit of guinea pig donor, under the experimental conditions described, even when performed for as long as 42 consecutive weeks, does not result in desensitization of the recipient to such grafts. Rather, such grafts continue to be rejected as white grafts. These results suggest that: (a) white graft responses may be an expression of a higher level of sensitivity than accelerated rejection, and (b) although white grafts do not become vascularized, they maintain the recipient's heightened state of homograft sensitivity to subsequent grafts from the same donor.

ISSN:0041-1337    

出版商:OVID    

年代:1966

数据来源: OVID

摘要:

Human blood platelets, while exhibiting some transplantation antigen activity, are not as potent in this regard as leucocytes. Dosages of 130–288 X 109platelets, obtained by repeated donor plasma-pheresis, induced homograft sensitivity in the recipients. Humoral and cellular responses noted after sensitization showed no direct correlation with the survival time of skin homografts in the same subjects.

ISSN:0041-1337    

出版商:OVID    

年代:1966

数据来源: OVID

摘要:

Urinary sediments, obtained from patients with renal allografts, were concentrated on Millipore filters. Cytological examination was performed at various intervals: in the immediate postoperative period, during clinically recognized episodes of graft rejection, and in periods of normal renal function. The urinary findings were related to renal biopsies. Renal tubular cells were present in the urinary sediment during the first 2 weeks after transplantation, usually in small numbers. Large numbers of tubular cells were present during acute rejection episodes, associated with extensive interstitial edema and tubular damage of the graft. Disruption of tubules and their infiltration by mononuclear cells was evident at that time. Renal tubular cells became infrequent during the recovery period. In the slowly progressive chronic rejection reactions, renal tubular cells were also present in the urinary sediments, but in only moderate numbers. Since cytological examination of urine sediments is readily accomplished and renal tubular cells are characteristic, this technique may be helpful in assessing threatened rejection.

ISSN:0041-1337    

出版商:OVID    

年代:1966

数据来源: OVID

ISSN:0041-1337    

出版商:OVID    

年代:1966

数据来源: OVID