摘要:

SUMMARYTen of 12 pigs did not reject renal allografts when treated with antilymphocyte globulin (ALG) daily for 10 days. Animals with functioning allografts were sensitized to alloantigens, as evidenced by the development of antikidney antibody and by accelerated rejection of skin allografts. Analyses of the immune status of these animals suggested that neither tolerance nor enhancement was responsible for graft survival. Possible mechanisms were discussed, with emphasis upon the uniqueness of the cellular immune response of this species and its low threshold for suppression.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYC57BL/6J lymphoid cells incubated in vitro with C3H/He macrophage monolayers for a short time are capable of causing accelerated C3H skin graft rejection when transferred to syngeneic recipients. For C57 lymphoid cells to transfer this “sensitization,” certain requirements of the in vitro system must be met. (1) In vitro exposure to C3H macrophages must be maintained for at least 1 hr. (2) At least 15 × 106lymph node cells need to be transferred to syngeneic animals. (3) C57‐C3H cell contact is necessary for sensitization to occur. It was also found that (1) the in vitro sensitization reaction that causes subsequent accelerated graft rejection is specific for the strain used as macrophage source; (2) prolonged cultures of macrophages may contain a factor inhibiting the sensitization process; and (3) lymphoid cells from different sources vary in their ability to transfer this sensitization. Bone marrow and peripheral leukocytes are more efficient than lymph node and spleen; thymus cells are least efficient.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYAn investigation was made on the effects of donor‐host cross circulation on skin homograft rejection in the rat, a test being made of various histocompatibility barriers: F1, (F/344 × Lew) → F/344, F/344 &lrarr2; Lew, F/344 &lrarr2; Bf. We obtained the most significant prolongation of graft survival by grafting hybrid F1(F/344 × Lew) skin on F/344 cross circulated with Lew 2 days before. Positive results were obtained with the combination Lew &lrarr2; F/344 if the F/344 or Lew skin grafts were carried out 2 days after cross circulation. No effect was observed if the grafts were performed 22 days later, preceded by the i.v. injection of 5 × 107donor spleen cells. The administration alone of 108spleen cells resulted in a fair degree of activity in the F/344 → Lew combination and not vice versa. The results obtained with the strong histocompatibility barrier were contradictory. There was a slight increase of the mean survival time (MST) in the F/344 → Bf combination; on the contrary, a sensitizing effect in the Bf → F/344 combination was observed. These results are discussed and interpreted on the basis of the hypothesis that other factors besides the exchange of antigens intervene in cross circulation.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYStrain B10.129(12M) is a congenic resistant strain differing from its partner strain, C57BL/10 or B10, by a rather weak histocompatibility locus. The locus is unusual in that the sex difference in the response to grafts is especially pronounced. Preimmunized females are strongly resistant to skin or tumor transplants; preimmunized males are not. Evidence presented herewith shows that B10.129(12M) identifies a new histocompatibility locus. This already has been provisionally designatedH‐12;this usage is now confirmed. The allele of B10.129(12M) is assigned the symbolH‐12b. Congenic resistant strain B10. 129(6M) is alsoH‐12bbut in addition carries a minorH‐2difference from B10. Strain B10 isH‐2b; strain B10. 129(6M) has a newH‐2allele, derived from the 129 parental strain, to which the symbolH‐2bcis assigned. Strain B10. 129(6M), unlike B10, also reacts with TL antisera. Presumably theTlaallele of 129, which determines the TL.2 antigen, was brought in with theH‐2allele to which it is closely linked.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYThe role of the thymus in the immunological maturation of mouse embryonic liver cells was studied, with the ability of the liver cells to cause a graft‐versus‐host reaction as the assay. Parental embryonic liver cells were tested for their ability to induce a graft‐versus‐host reaction in 1‐day‐old normal or neonatally thymectomized F1mice. Splenomegaly induction in this instance was demonstrable, although weak. The response in thymectomized hosts was as good as that in normal hosts. Parental embryonic liver cells were injected into normal or thymectomized semiallogeneic F1irradiated animals whose spleens were subsequently removed and tested for their ability to initiate an in vitro graft‐versus‐host reaction in syngeneic (F1) test explants. Positive responses were induced by spleens from both normal and thymectomized hosts, but there was considerable delay with spleens from the latter group. Since the only allogeneic cells in this system were liver‐derived, it is concluded that immunological maturation of embryonic liver cells, at least in the splenic environment, is thymus‐influenced but not entirely thymus‐dependent.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYThe distribution kinetics of i.v. injected51Cr‐labeled CBA lymph node cells were studied in normal CBA mice and in mice subjected to various types of lymphoid depletion. Cells from normal animals were transiently delayed in the lungs and spleens of normal recipients and accumulated gradually in lymph nodes during the first 24 hr. The proportional distribution of labeled lymphocytes was similar over a cell dose range of 105‐108/recipient. The proportion of donor lymph node cells capable of “homing” to lymph nodes of normal recipients was greatly reduced by the following donor treatments: neonatal thymectomy, adult thymectomy, irradiation, syngeneic bone marrow reconstitution, or prolonged drainage of the abdominal lymphatic duct. Exposure of donors to 600 R total body X‐irradiation curtailed migration of donor cells to both lymph node and spleens of recipients, suggesting that more than one population of donor lymphocytes was affected. Donor treatment with repeated closes of heterologous antilymphocyte serum (ALS) profoundly and preferentially affected recirculating lymphocytes, reducing the representation in donor lymph nodes to levels much below those seen after neonatal thymectomy or thoracic duct drainage. The syngeneic51Cr‐labeled cell transfer assay may provide a means of analyzing the action of immunosuppressive agents in terms of their effects on the distribution of lymphoid cell subpopulations.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYCardiac dimensions were measured serially using recently developed ultrasonic echographic techniques in 12 patients after orthotopic cardiac allotransplantation. Changes in the echographic measurement were noted in 20 of the 26 episodes of acute rejection that were diagnosed by other methods during the period of study. Increased posterior left ventricular wall thickness and right ventricular dilation were the changes most often observed during acute rejection, and these changes were reversed by successful immunosuppressive therapy. While false positive diagnoses were noted relatively frequently by the echographic criteria employed in this study, this did not detract significantly from the clinical utility of the technique. Directional changes in cardiac dimensions noted with echography are consistent with the known histopathological and pathophysiological changes in the cardiac graft during acute immunological rejection. The echographic technique used in this study is atraumatic and easily repeatable, suggests early stages of the rejection process, and is moderatelv reliable in indicating rejection.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYThe donor‐type lymphocytes which mediate a graft‐versus‐host reaction (GVHR) in F1hybrid rat kidney were shown to receive fully effective immunogenic stimulation by foreign H antigens of radiosensitive leukocytes of the host. Under the conditions of the experiments, no evidence was obtained for immunogenicity of cell‐free H antigens in the tissue lymph or of H antigens present upon cells indigenous to the kidney, i.e., parenchymal or nonleukocytic stromal cells. The proliferation of lymphocytes from Lewis rat donors was inhibited, as was their ability to generate a local invasive destructive lesion, following their injection under the kidney capsule of (L × BN)F1hybrid hosts which had been previously rendered leukopenic by whole body irradiation. A similar result was achieved if the hematopoietic tissue of the F1 hosts had been repopulated with Lewis bone marrow cells. On the other hand, lymphocytes from Lewis donors proliferated and induced renal graft‐versus‐host (GVH) lesions, comparable in intensity to those in controls, in the kidneys of Lewis rats repopulated with (LBN)F1hematopoietic cells. Evidence was also obtained which suggests that the failure of Lewis cells to respond in the (LBN)F1hosts, repopulated with Lewis marrow, could be attributed at least in part to the presence of immunosuppressive factors in these chimeras.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID