摘要:

SUMMARYMarrow transplants were carried out between unrelated donor-recipient pairs of dogs that were homozygous and identical for DLA-A, B, C, and D, i.e., mutually nonreactive in mixed leukocyte culture. Recipients were conditioned for transplantation by 1,200 R of total body irradiation and then treated with intermittent methotrexate for 102 days in order to prevent or delay graft-versus-host disease (GVHD). Of 13 dogs that received transplants, 4 are surviving with good grafts and no GVHD for more than 12 to 20 months. Nine died, 6 with GVHD between days 26 and 141, 1 with wasting on day 65, 1 with interstitial pneumonia on day 83, and 1 with graft rejection on day 23. In comparison, the survival of 17 DLA-identical littermates treated in the same manner was significantly better with 16 surviving without GVHD (P < 0.01), while the survival of 54 DLA-nonidentical littermates was significantly worse with only two surviving without GVHD (P < 0.025).These results are incompatible with the concept that solely the loci detected by mixed leukocyte culture and serotyping are responsible for GVHD. One or more additional loci appear to be involved. Knowledge of this locus (loci) is important if marrow grafting between unrelated individuals is to be successful. However, results also indicate that an unrelated “compatible” marrow graft is more likely to succeed than a graft from an incompatible littermate.

ISSN:0041-1337    

出版商:OVID    

年代:1977

数据来源: OVID

摘要:

SUMMARYAcute antibody-mediated rejection (AAR) of mouse skin allografts was studied in nine donor-recipient combinations with increasing histoincompatibility ranging from an H-Y to a complete H-2 plus non-H-2 disparity. AAR was induced by the injection of specific alloantiserum along with a heterologous complement on day 7 after grafting. Sera from rabbits, guinea pigs, and from a human volunteer were used as complement sources. The recipients were treated with antilymphocyte serum on days 0, 2, and 4 to postpone cell-mediated rejection. With increasing histoincompatibility the mean survival time of untreated grafts decreased, the in vitro cytotoxic activity of the alloantiserum rose, and AAR could be induced with-lower amounts of antiserum. The higher efficiency of rabbit complement compared with guinea pig complement and human complement, that is known to exist in in vitro cytotoxicity, was also found in vivo. Rabbit complement could induce AAR in combination with relatively weak histoincompatibility (H-2K, H-2D, or non H-2 differences), where guinea pig complement and human complement were ineffective. All three complement species elicited AAR if there was a disparity for H-2D plus non-H-2, H-2K plus non-H-2, H-2, or H-2 plus non-H-2. The rules for immunogenicity of the different histocompatibility loci as they have been described for cell-mediated graft destruction also apply to this humorally mediated rejection process.

ISSN:0041-1337    

出版商:OVID    

年代:1977

数据来源: OVID

摘要:

SUMMARYForty-seven allophenic mice of three different types (C57BL/6 (A SJL), C57BL/6 (CBA CBA/H-T6), and C57BL/6 DBA/1) were analyzed for changes in their peripheral white blood cell composition and hemoglobin composition with age. It was found that 10 of the 47 mice showed significant changes termed “chimeric drift” in one or the other or both of these parameters. These 10 mice were classified as unstable chimeras, as opposed to the 37 stable chimeras, which showed no apparent chimeric drift. There was an excellent correlation of peripheral white blood cell and hemoglobin compositions of the stable chimeras. However, the unstable chimeras showed little or no correlation of these two markers. Possible mechanisms of chimeric drift are discussed.

ISSN:0041-1337    

出版商:OVID    

年代:1977

数据来源: OVID

摘要:

SUMMARYA series of seven animals underwent allotransplantation of a lung from a donor matched for the recognition of serologically defined (SD) antigens by the use of alloantisera followed by autotransplanation of bone marrow without further immuno-suppression. In two animals, the lung was transplanted into the recipient immediately before the administration of total-body irradiation and in five, the lung was transplanted after completion of total-body irradiation. In the first group, one animal is living at 46 months with good function of the transplant. The other was killed at 6 months with chronic rejection. In the second group, three of five animals are alive 46 to 47 months after transplantation. Two others died early, one of cardiac tamponade and the other of diffuse hemorrhage before engraftment of marrow could be accomplished. Significant function of the transplant was demonstrated in all long-term survivors.Four control animals, subjected to marrow harvest and reimplantation of the left lung followed by total-body irradiation and marrow reinfusion, were studied to determine the possible deleterious effects of the total-body irradiation and marrow transplant procedure. Pulmonary function studies showed some reduction in ventilation and perfusion to the upper lobe areas, which was probably related to technical factors but did demonstrate no severe adverse affects to the procedures.Autologous bone marrow reconstitution after total-body irradiation combined with allotransplantation of the lung has allowed long-term survival and function of lung transplants without the necessity for administration of immunosuppressive drugs after the transplantation procedure.

ISSN:0041-1337    

出版商:OVID    

年代:1977

数据来源: OVID

摘要:

SUMMARYUsing the Marbrook culture system, it was established that stimulation of H-2k(CBA) lymphocytes by H-2b(C57BL/6) spleen cells induced a population of cytotoxic lymphocytes (CL) capable of lysing H-2d(P815) target cells via public antigens common to H-2band H-2dhaplotypes. In contrast, no cross-reactive lysis of H-2b(EL4) target cells was observed following stimulation by H-2dspleen cells under identical conditions. Experiments were conducted which eliminated the possibility that this non-reciprocity of cross-reaction was caused by a failure of the H-2btarget, EL4, to express the common antigens. By competitive inhibition assays H-2band H-2dspleen and tumour cells were shown to contain the common antigens in comparable quantities, and yet H-2dspleen cells failed to induce cross-reactive CL when tested at up to 1,000 times the concentration needed for induction by H-2bspleen cells. Culture conditions were altered causing a boost in cytotoxic activity and enabling the detection of a cross-reactive response induced by BALB/c (H-2d) spleen cells. However, the degree of cross-reaction observed was still weaker than that induced by C57BL/6 (H-2b) spleen cells under identical conditions. It was concluded that the common public antigens, although shown to be expressed in equivalent concentrations on the H-2band H-2dtissues examined, were relatively poor immunogens when presented on H-2dcells (BALB/c spleen and P815 tumour), as measured by their ability to induce a clone of CL directed against them. The implications of these results for expression of receptors on CL and their precursors are discussed.

ISSN:0041-1337    

出版商:OVID    

年代:1977

数据来源: OVID

摘要:

SUMMARYThe alloantigen reactivity of spleen and lymph node cells from specifically sensitized (MHA anti-CB) hansters is analyzed in graft-versus-host reactions (GVHR) and mixed lymphocyte reactions (MLR). Analysis of GVHR in appropriate F1hybrid animals revealed that parental MHA anti-CB hamster lymphoid cells elicit a significantly greater local GVHR than normal unsensitized cells. This pattern of response was observed for hyperimmune lymph node cells (LNC) and for spleen and LNC various times after a single s.c. inoculation of allogeneic (CB) lymphoid cells.This unrestricted clonal expansion was only partially reflected in the MLR. Seven days after a second inoculation of CB lymphoid cells, MHA anti-CB LNC demonstrate a dramatic increase in proliferative capacity over that evoked by unsensitized cells. This was also true for MHA anti-CB spleen and LNC 7 days after a single immunization regime. However, both spleen and LNC demonstrate a delayed onset of clonal proliferation. The immune cell response for both populations is less than that observed for unsensitized cells between 3 and 5 days postimmunization.The unrestricted clonal expansion of hamster lymphoid cells in GVHR and MLR to allogeneic antigens is discussed in terms of the current understanding of the immuno-genetics of the hamster major histocompatibility complex, and the capacity of this species to regulate conventional immune responses.

ISSN:0041-1337    

出版商:OVID    

年代:1977

数据来源: OVID

摘要:

SUMMARYIsogeneic bone marrow transplantation was performed in “nude” and normal C3H mice after lethal doses of dimethylmyleran (DMM), meso-DMM, and ()-DMM were given. Nude mice were not more sensitive to DMM or its isomers than normal mice. With adequate cell doses in the range of 3 106to 30 106bone marrow cells or 10 106to 30 106spleen cells, the survival rate after lethal chemotherapy was not significantly different between nude mice reconstituted with a graft from nude donors and normal mice reconstituted with a graft from normal donors. Only with suboptimal cell doses (1 106bone marrow cells) was survival superior in normal mice. The hemopoietic capacity was not conferred to the nude donor cells by an extrinsic factor. Nude mice accepted also bone marrow grafts from allogeneic nude donors.

ISSN:0041-1337    

出版商:OVID    

年代:1977

数据来源: OVID

ISSN:0041-1337    

出版商:OVID    

年代:1977

数据来源: OVID

ISSN:0041-1337    

出版商:OVID    

年代:1977

数据来源: OVID

ISSN:0041-1337    

出版商:OVID    

年代:1977

数据来源: OVID