摘要:

SUMMARYKidneys were transplanted between inbred strains of rats, and the renal interstitium was examined serially by light, immunofluorescence, and electron microscopy. In addition to observations reported by various authors previously, several observations not previously described or emphasized were made during this study. They include direct cytoplasmic communications between mononuclear cells, prominent replication of mononuclear cells within the allograft, and abundant collagen production within the allograft. No morphological evidence was found during this study to support the view that the mononuclear cells destroy the renal parenchyma by antibody-mediated mechanisms or by contact-mediated cytotoxicity.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYChanges in cell-mediated hypersensitivity to liver and histocompatibility antigens have been detected, by means of the leucocyte migration test, during rejection of liver transplants. Of the 10 cases studied after an orthotopic liver graft, five developed acute episodes of rejection which were of varying severity. In these five cases, inhibition of leucocyte migration was found to be coincident with deterioration in liver function tests and with the histological appearances of rejection on liver biopsy. Two cases developed a cholestatic jaundice, probably not attributable to rejection, and inhibition of leucocyte migration was not found. Chronic rejection, which developed in two patients, was associated with inhibition of migration in one but not in the other. The leucocyte migration test is therefore of most value in the diagnosis of acute rejection.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYIn mice treated with antilymphocyte serum (ALS), the curve relating dose of serum to number of antibody-forming cells or graft-rejecting cells is hyperbolic, i.e., log cell number is inversely related to log dose. As a consequence, it is shown on theoretical grounds that, if a fixed total amount of serum is administered over a fixed total period, regimens of small frequent doses should be, within limits, more effective than regimens of large, infrequent doses. This prediction has been confirmed by comparing the effects of different regimens of ALS on the formation of hemolytic plaque-forming cells (PFC) in the mouse spleen after injection of sheep red cells and on rejection of skin grafts.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYThe mechanism of active immunological enhancement of skin allografts was studied in CBA/J mice immunized with lyophilized spleen tissues from A mice by means of passive and adoptive transfer of immunity. The results of adoptive transfer of immunity revealed the significance of inhibition of the cell-mediated response in actively immunized mice. Hydrocortisone injected at an appropriate time led to a further significant prolongation of skin allograft survival in both the actively and passively immunized animals.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYThe local graft-versus-host (GVH) reaction reported by Ford et al. (4) has been applied to a murine model. In principle, the increase in number of nucleated cells contained in the popliteal lymph node of an Fi hybrid was determined after regional injection of immunocompetent parental strain spleen cells. This assay was used to test cell-mediated immune reactivity of spleen cells from casein-treated mice at various stages of amyloid induction. Concurrently, the test was applied to casein-treated donor mice presensitized with 2 consecutive allogeneic (second parental strain) skin grafts. The number of cells in the lymph nodes showed a linear relationship to logarithmically spaced numbers of cells injected. Marked depression of the GVH reaction was observed with spleen cells from nonsensitized donors treated with 10 and 20 daily injections of casein. In contrast, 10 injections of casein in the presensitized group did not affect the GVH reactivity of donor spleen cells. After 20 casein injections the same high degree of depression was encountered with spleen cells from both grafted and nongrafted groups of donor mice. In the presensitized and nonsensitized groups the same histological changes occurred under casein treatment, i.e., 10 casein injections gave rise to perifollicular pyroninophilia and 20 injections caused perifollicular amyloid deposits. The results are suggestive of cellular immune depression attributable to amyloid formation.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYA histochemically active population of macrophages has been demonstrated in the cellular infiltrate that occurs as an immunological response to skin allografts in mice. The proportion of these active cells was found to increase to a peak of 50% 10 days after the application of the graft. The time required to demonstrate particulate acid phosphatase activity in these macrophages was shown to decrease, up to and over the point of graft rejection. Concurrent studies of lysosomal proteolytic activity and lysosomal permeability have led to the conclusion that a buildup of cells having very active lysosomal enzymes in the infiltrate could be, in part, responsible for the rejection of the donor skin.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYLymph node and spleen cells from AS rats bearing enhanced (August X AS)Fi kidneys were tested for their capacity to produce a local graft-versus-host reaction (GVHR), using the Elkins' technique and the popliteal lymph node assay. It was found that lymphoid cells from rats bearing enhanced kidneys were able to mount GVHR quantitatively similar to those caused by cells from control rats. Immunisation of rats bearing enhanced kidneys with August spleen cells caused a rise in cytotoxic antibody titre but had no effect on blood urea levels. Lymphoid cells taken from these additionally immunised rats also produced GVHR comparable with those of controls. It is concluded that tolerance is not involved in the prolonged survival of these grafts.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYImmunosuppressive sera have been produced by injecting rabbits with soluble extracts isolated from mouse thymocytes. In vitro properties of these antisera are compared with in vivo immunosuppressive activities. Rabbit antisera to soluble extracts from human lymphocytes have also been prepared. Some of these antisera showed in vitro properties suggesting they would be immunosuppressive in man. The soluble lymphocyte extracts have been examined by immunodiffusion and gel electrophoresis.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYIt is shown that the administration of a course of antilymphocytic globulin (ALG) significantly decreased the efficacy of a subsequent course in prolonging allograft survival. It was also found that survival of a second allograft was significantly less in rats treated with ALG at the time of this graft whether, at the time of the primary graft, they were or were not given ALG.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYProlongation of survival time of cardiac allografts in rats by the previous i.v. injection of donor strain blood is described. Modest prolongation of survival time of split skin grafts is also demonstrated. A similar effect on cardiac allografts occurs when donor strain thoracic duct lymphocytes are employed instead of blood. Donor strain plasma and red cells are shown to be ineffective. Passive transfer of serum from recipient strain animals “conditioned” with donor strain blood does not produce prolongation of survival time of cardiac allografts. Perfusion of the coronary arteries with such serum before transplantation also has no effect. Rats with long surviving cardiac allografts are shown to reject donor strain split skin grafts in a manner similar to that of control rats.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID