摘要:

The purpose of this investigation was to examine the interaction between transplanted tooth buds and bone tissue. The buds of the first maxillary molars from 10-day-old rats were autotransplanted into the dorsal connective tissue, either alone, together with alveolar bone, or together with a piece of the tibia; for comparison, tooth buds were also transplanted into the tibia in situ. No deleterious effects on the buds were noticed when transplanted together with alveolar bone. However, massive resorption and creeping replacement of the transplants were seen when transplanted together with bone from the tibia. The same was true for the buds transplanted into the tibia. Alveolar bone seems to have different inductive and protective properties with regards to the tooth bud than tibial bone, and appears to be a more physiologic site for tooth development.

ISSN:0041-1337    

出版商:OVID    

年代:1967

数据来源: OVID

摘要:

A panel of isoantisera, prepared by injecting rabbit spleen cells and adjuvant was used to classify the leucocyte antigens of 52 rabbits. Blood leucocytes from each animal were incubated‘ with each antiserum and the damage was assessed by the eosin dye exclusion method. Most antisera were of narrow specificity and appeared to demonstrate antigens controlled at one genetic locus only. There were at least 3 alleles which could occupy this locus. Each possible donor-host pair within batches of 7 to 12 animals was given an index number calculated from a comparison of the susceptibilities of the donor's and host's leucocytes to the cytotoxic action of the antiserum panel. The rabbits were then grafted with the skin of a number of others in the batch. The rejection orders of 137 grafts on 43 recipients were compared with serological assessment of their similarity. In 70% of cases the grafts from the donors which had been indicated as most similar to their hosts survived as long or longer than any other on that recipient. There was a significant positive correlation between the predicted and the observed rejection orders (P≥ 0.018). This suggested that the locus which had been serologically demonstrated was strongly concerned in histocompatibility. When each animal was assigned a phenotype according to the alleles of this locus some recipients bore grafts from compatible and incompatible donors. In 13 of the 14 relevant cases a graft which was compatible lasted as long or longer than the incompatible grafts on the same individual. It is suggested that panels of cytotoxic antisera may be of value in histocompatibility testing.

ISSN:0041-1337    

出版商:OVID    

年代:1967

数据来源: OVID

摘要:

Five Leydig cell tumors from BALB/c mice and 2 mammary gland tumors, one of C3H and the other of (C3H x A)Fzorigin, have manifested progressive growth in untreated allogeneic DBA/1 hosts following a 6–28-day maintenance period in vitro by the organ culture explantation technique. Noncultured control implants of these same tumors failed to grow in similar recipients. In addition, a mammary tumor of DBA/1 origin showed delayed rejection in BALB/c recipients following organ culture maintenance. Three rat tumors also have grown in allogeneic recipients following a single organ culture passage. Four of the Leydig cell tumors now have been passed serially through DBA/1 hosts, and one of these for as many as 12 transfer generations. These tumors maintain their ability to grow in BALB/c mice and after being returned to this strain for one passage lose their ability to grow in allogeneic DBA/1 hosts. Thus, it is concluded that the maintenance in vitro repressed or reduced the antigenicity of these tumors and that this effect was reversed by a single passage back into the isogeneic strain. After culture these tumors maintain their morphologic and functional characteristics in both the isogeneic and allogeneic recipients.

ISSN:0041-1337    

出版商:OVID    

年代:1967

数据来源: OVID

摘要:

The histological and histochemical findings in 18 canine renal homografts are discussed. The alkaline phosphatase isoenzyme pattern was determined in serial urine and serum specimens of 6 homografted dogs dying because of an irreversible rejection crisis. The rejection process is arbitrarily divided into two phases: a cellular and a humoral attack on the graft. The cellular attack, which is directed against the peritubular capillaries, gives rise to ischaemic tubular damage. This is reflected in an increase in urinary renal alkaline phosphatase, in a decrease in proximal tubular esterase and, often, in an increase in proximal tubular glucose-6-phosphate dehydrogenase activity. The humoral attack (a rejection crisis), presumed to be due to circulating antibody or antigen-antibody complexes being deposited in the glomerular capillaries, results in increased glomerular permeability. The increase in glomerular alkaline phosphatase, the redistribution of proximal tubular acid phosphatase, and the appearance of liver alkaline phosphatase isoenzyme in the urine are secondary to the increase in glomerular permeability.

ISSN:0041-1337    

出版商:OVID    

年代:1967

数据来源: OVID

摘要:

Murine marrow cells which have been frozen and thawed were studied for their ability to differentiate and proliferate following transplantation into isogeneic hosts. Using the hematopoietic colony technique, these cells were compared with fresh cells by injecting them into irradiated CAF1mice. Serial sections of hematopoietic colonies produced in the animals' spleens were studied, and the volume of the largest erythroid colony in each of these spleens was measured. The pattern of cellular differentiation and the volume of the erythroid colonies produced by thawed cells were found to be similar in every respect to those produced by fresh cells, suggesting that freezing and thawing does not affect the transplantation ability of the hematopoietic stem cells which survive these processes.

ISSN:0041-1337    

出版商:OVID    

年代:1967

数据来源: OVID

摘要:

In order to investigate whether the fetal membranes of the mouse possess properties equipping them to form part of the barrier to transplantation immunity between fetus and mother, fetal membranes were grafted upon skin graft beds and overlaid with skin isogeneic to the adult host. When the inner surface of the membrane was down, grafts adhered to the bed, were promptly vascularized, and survived in good condition for 10 days. When the outer membrane surface was down, a cavity developed between membrane and bed, vascularization was delayed and occurred only from the cut edges of the graft, and the membrane epithelium degenerated after 6 days. Regardless of membrane orientation, brisk allograft reactions took place in pre-sensitized allogeneic hosts, demonstrating the presence of transplantation antigens in the fetal membranes. The resistance of the outer membrane epithelium to vascularization and its tendency to secrete amorphous material which created a cavity between it and the graft bed may be among the properties which normally prevent invasion of the fetal membranes by maternal blood vessels and the consequent wholesale exchange of transplantation antigens.

ISSN:0041-1337    

出版商:OVID    

年代:1967

数据来源: OVID

摘要:

To evaluate the most efficient method for preparation of single cell suspensions from viable tumor tissue for use in in vivo experiments, a mouse methylcholanthrene-induced sarcoma and a polyoma virus-induced sarcoma were used. The tumor cells were disassociated by four different methods and the state of viability, yield of single cells per 0.1 gram of tumor, and the quantity of cell debris were determined. The effect on transplantability of each of the methods was assessed by injection s.c. of a constant number of viable cells. Of the four methods tested, the cell preparation resulting from treatment of the tumor with the proteolytic enzyme “Pronase” yielded the highest number of viable cells and the smallest quantity of cell debris. Transplantability of the tumor cell suspension prepared by each of the methods was similar when a constant number of viable cells was injected.

ISSN:0041-1337    

出版商:OVID    

年代:1967

数据来源: OVID

摘要:

Some of the physical and chemical properties of blastogenic material contained in cell-free culture medium of 5-day mixed leukocyte cultures have been described. These properties seem similar in many respects to those of dog and mouse transplantation antigens which have been described by other workers. Characterization and purification of the blastogenesis factor may lead to increased understanding of transplantation immunity. In view of the relatively stable qualities of this factor, it may be possible to concentrate it in sufficient quantity to have practical application in tissue matching.

ISSN:0041-1337    

出版商:OVID    

年代:1967

数据来源: OVID

摘要:

A quantitative method for the assay of transplantation antigens in mice differing at theH-2histocompatibilty locus is described. The technique, based on principles elaborated earlier by Medawar, measures directly the ability of cells, cellular fractions, or other substances to induce in the recipient a response which leads to the accelerated rejection of a test skin allograft. Histologic criteria are given for the scoring of the epithelial survival of the test grafts. By using scores based on these criteria, linear dose-response regressions have been obtained with transplantation antigens administered either as whole cells derived from one of the four lymphoid cell-containing tissues (spleen, bone marrow, lymph nodes, and thymus), or as freeze-dried fractions of cells. The reproducibility and specificity of the assay are denned. The assay has utility in the detection of transplantation antigens as well as in the study of their properties.

ISSN:0041-1337    

出版商:OVID    

年代:1967

数据来源: OVID

摘要:

New data are presented concerning a blood group locus of mice originally identified by Hoecker and co-workers. Specificity “R”, best defined by a (BIO X LP.RIII) anti-RIII, and specificity “Z”, best defined by an RF anti-C3H, are shown to be antithetical. Specificity R is present in strains RF, RIII, and F, absent from all other strains tested; specificity Z is absent from RF, RIII and probably F, and present in all other strains tested. Tests in a segregating generation show that R and Z are determined by alleles at a single locus. The locus is distinct from and probably not linked withH-2, though distorted ratios have been observed in certain cases. Evidence with respect to distinctness from other histocompatibility and blood group loci is incomplete, though there is a considerable presumption that it is not the same as any of the 10 loci identified by strictly histogenetic methods. Strong anti-R sera are easily prepared; specificity Z is somewhat weaker.

ISSN:0041-1337    

出版商:OVID    

年代:1967

数据来源: OVID