|
11. |
Sensitivity of IgG-ELISA for Detecting Airborne Pollen Antigens of Betulaceae |
|
Grana,
Volume 34,
Issue 5,
1995,
Page 350-356
E. Pehkonen,
A. Rantio-Lehtimäki,
Preview
|
PDF (735KB)
|
|
摘要:
The reasons for the occurrence of high birch pollen antigen concentrations in the air, especially in small particle sizes and before the birch flowering period, have been closely studied. Some particles with pollen antigenic activity are known to be emitted from other parts of the tree in addition to the catkins. Pollen from related species which flower before the birch, or simultaneously with it, may provide another explanation for these antigen peaks. In the study reported here, birch pollen antigenic activity before flowering was analysed in 1993 and 1994 using IgG-ELISA with polyclonal antibodies. A clear indication of the emission of small micronic particles with pollen antigenic activity before birch flowering was a peak before and during the pollen period of alder. Alder is the first tree to flower in the spring in Finland. Because of the high homology in the major allergens of the family Betulaceae, either polyclonal antibodies against birch pollen or monoclonalBet v Ican be recommended for both aerobiological monitoring and information service.
ISSN:0017-3134
DOI:10.1080/00173139509429070
出版商:Taylor & Francis Group
年代:1995
数据来源: Taylor
|
12. |
Aeroallergen Immunoblotting with Human IgE Antibody |
|
Grana,
Volume 34,
Issue 5,
1995,
Page 357-360
Y. Takahashi,
S. Nilsson,
B. Berggren,
Preview
|
PDF (425KB)
|
|
摘要:
A new method is presented for the identification of the causative pollen of pollinosis patients. The airborne sample collected with a Burkard sampler was cut into two equal halves lengthwise. One was treated with the pollinosis patient's IgE using an indirect immunoenzymatic technique applied avidin-biotin system. The other half of the sampling tape was stained with basic fuchsin solution and the major pollen types were counted morphologically. The spot obtained and individual pollen counts were compared consecutively, and the causative pollen for the patient was estimated. Furthermore, verification was performed using the reference pollen of the estimated causative pollen. The method does not require any diagnostic extracts and needs only a small number of intact pollen grains. It is suitable for the identification of the causative pollen in rare pollinosis cases and the pollen for which a diagnostic extract is not commercially available.
ISSN:0017-3134
DOI:10.1080/00173139509429071
出版商:Taylor & Francis Group
年代:1995
数据来源: Taylor
|
13. |
Ninth International Palynological Congress |
|
Grana,
Volume 34,
Issue 5,
1995,
Page 361-361
Preview
|
PDF (102KB)
|
|
ISSN:0017-3134
DOI:10.1080/00173139509429072
出版商:Taylor & Francis Group
年代:1995
数据来源: Taylor
|
14. |
Editorial board page for “Grana”, Volume 34, Number 5 |
|
Grana,
Volume 34,
Issue 5,
1995,
Page -
Preview
|
PDF (94KB)
|
|
摘要:
This is a scanned image of the original Editorial Board page(s) for this issue
ISSN:0017-3134
DOI:10.1080/00173139509429059
出版商:Taylor & Francis Group
年代:1995
数据来源: Taylor
|
|